HPLC
Hemoglobin Analyzer
BD dept.
2025.06
Overview
1 HbA1c & HPLC
Introduction-
CONTENTS 2 Wondfo HPLC Hemoglobin Analyzer
3 Main Player
PART 1
Overview — HbA1c & HPLC
What is HbA1c?
HbA1c, also known as glycated hemoglobin, is a compound produced when blood glucose
chemically links to hemoglobin in red blood cells (RBCs). The formation of HbA1c is proportional to the
blood glucose concentration. HbA1c testing reflects the average amount of blood glucose level over
the preceding 2-3 months regardless of instantaneous fluctuation of blood sugar.
Hemoglobin
Glucos
e
Glucose Glycated Hemoglobin
The glycosylation process is irreversible, and glycated hemoglobin remains glycated during the life
time of RBCs. Therefore, HbA1c can indicate the average blood sugar level of 2-3 months because the
RBC lifespan is 8 to 12 weeks.
What is Diabetes?
Diabetes mellitus (DM), commonly known as diabetes, is a group of metabolic
disorders characterized by a high blood sugar level over a prolonged period of time.
Severe diabetes leads to multiple complications .
Type 1 diabetes
Diabetes
Type 2 The most
diabetes prevalent form
Gestational
diabetes
Other types of
diabetes
A1c & Diabetes
WHO: HbA1c- Diabetes diagnostic
standard
Normal High Diabet
Risk es
A1C ＜ 5.5~6.4 6.5%
5.5% %
HbA1c diagnostic standard
The International Federation of Clinical Chemistry and laboratory
medicine (IFCC) and the National Glycohemoglobin Standardization
Program (NGSP) use high performance liquid chromatography
(HPLC) as the gold standard for diagnosing HbA1c.
HbA1c HPLC
GOLD STANDARD
+ GOLD
STANDARD
Principle of HPLC hemoglobin detection
Hemoglobin types: Result ：
SA1C A
A1 0
b LA1C
+
Hb
A1
F
a
Process: The core principle is that different
components have different elution
Elution Buffer 1/2/3 times in elution buffers of different
（ Different density ） Chromatography column concentrations.
Ion exchange method. Different Detector Result
eluent concentrations result in
Samples different elution times for each (optical detection)
component
PART 2
Introduction —
Wondfo HPLC Hemoglobin Analyzer
Wondfo HPLC Hemoglobin Analyzer
Eluent buffer Hemolysis
1/2/3 Reagent
P10 P6
0 Analytical Column Control&Calibrator
Features
SMALLER, FASTER, SIMPLER
Detection Speed Standerd Mode: 60s/test Variant Mode: 96s/test
Sample Loader 10
HbA1c, HbF, HbA1a, HbA1b, LA1c, HbA0, HbC, HbD, HbE, HbS, HbA2
Separation Component
Sample Type Whole blood/ Hemolysis
Repetability CV≤1%
Test Range 3%~20%
Operation Interface 10.4 inches touch screen
P10 Dimension 575(L) mm X 350(W) mm X 545(H) mm
Weight 35 kg
Features
EFFICIENT, PRECISE, EXPERT
Detection Speed Standerd Mode: 60s/test Variant Mode: 96s/test
Sample Loader 51 (50 automatic sample positions, 1 emergency position)
Separation ComponentHbA1c, HbF, HbA1a, HbA1b, LA1c, HbA0, HbC, HbD, HbE, HbS
Sample Type Whole blood/ Hemolysis
Repetability CV≤1%
Test Range 3%~20%
Operation Interface 10.4 inches touch screen
P60 Dimension 691(L) mm X 555(W) mm X 560(H) mm
Weight 55 kg
HIGH-PRECISION
Accurate and stable
not affected by the dilution ratio and sample
CV ≤
sedimentation
1%
Excellent correlation with mainstream
product
RELIABLE RESULT
A0 Be able to identify the most common
SA1C glycosylated hemoglobin variants
A1b
LA1C+
A1a HbF
High resolution with sharp and independent
peaks
Report IFCC and NGSP results simultaneously
HIGH PERFORMANCE CHROMATOGRAPHIC COLUMN
High density
gel
High Performance Column
• Self-produced
• Long lifespan: 2000 tests (or 24months)
• Easy to store: 4-30℃
Patented gel filling
technique
USER-FRIENDLY
• One click to start testing, No sample pre-
treatment
• Automatic calibration and control process
• Chromatographic column can be replaced easily
• Automatically identify sample types and
support bidirectional LIS system
• Consumables are managed with RFID card to
avoid reagent errors
APPLICATION SCENARIO
Central Laboratory Diabetes Center General Hospital
Laboratory
Clinic GP office
PART 3
Comparision
WONDFO P10 VS OTHER
BRAND
THROUGHPU SAMPLE
BRAND MODEL MODE MEASURED ITEMS TEST TIME COLUMN LIFESPAN MANTAINANCE DIMENSION/WEIGHT STORAGE CV RESULT
T VOLUMN
HbA1c, HbF, HbA1a,
standard
HbA1b, LA1c, HbA0, W350×L575×H545
Wondfo P10 / 10 60s/test >2000 tests 12 months 3 μL 100,000 ≤1%
HbC, HbD, HbE, 35kg
variant
HbS, HbA2
HbA1c, HbF, HbA1a,
standard
HbA1b, LA1c, HbA0, 10/50 W402×L534×H495 10,000 ～
Bio-Rad D10 / 180s/test 500~1000 tests 6 months 4 μL ≤1%
HbC, HbD, HbE, (optional rack) 30kg 15,000
variant
HbS, HbA2
standard HbA1c, HbF, HbA1a,
HA- W330×L515×H485
ARKRAY / HbA1b, LA1c, HbA0, 10 100s/test 2000 ～ 3000 tests 12 months 4 μL 10,000 ≤1.5%
8380V 28kg
variant HbC, HbD, HbE, HbS
HbA1c, HbF, HbA1a,
standard
Lifotroni HbA1b, LA1c, HbA0, W360×L540×H600
H8 / 10 130s/test 1000 ～ 1500 tests 6 months 5 μL 4,000 ≤1.5%
c HbC, HbD, HbE, 36.8kg
variant
HbS, HbA2
HbA1c, HbF, HbA1a,
standard
MEDCON HbA1b, LA1c, HbA0, W360×L540×H600
MQ3000 / 18 115s/test 800/1600/2400 tests 6 months 4 μL 100,000 ≤2%
N HbC, HbD, HbE, 36.8kg
variant
HbS, HbA2
WONDFO P10 VS OTHER
BRAND
Bio-rad
D10
ARKRAY Fast detection speed
HA-8380V
Long lifespan column
Small amount of sample volumn required
High resolution with sharp and independent
LIFOTRONIC
H8 peaks
Powerful storage capacity
Wondfo P10
MEDCONN
MQ3000
WONDFO P60 VS OTHER
BRAND
COLUMN MANTAINANC SAMPLE
BRAND MODEL MODE MEASURED ITEMS THROUGHPUT TEST TIME STAT DIMENSION/WEIGHT STORAGE CV RESULT
LIFESPAN E VOLUMN
HbA1c, HbF, HbA1a,
standard/ L691 x W555 xH560
Wondfo P60 HbA1b, LA1c, HbA0, 50+1 (STAT) 60s/test √ >2000 tests 12 months 3 μL 100,000 ≤1%
variant 55kg
HbC, HbD, HbE, HbS
HbA1c, HbF, HbA1a,
standard/ L650 x W660 xH725
Bio-Rad D100 HbA1b, LA1c, HbA0, 100+3 （ STAT ） 45s/test √ 1500 tets 6 months 4 μL 15,000 ≤1.7%
variant 95kg
HbC, HbD, HbE, HbS
HbA1c, HbF, HbA1a,
standard/ HbA1b, LA1c, HbA0, 90/290+1 L530 x W515 xH482
Tosoh G8 96s/test √ 1500 tets 12 months 4 μL >800 <2%
variant HbC, HbD, HbE, HbS, (STAT) 34kg (90 samples)
HbA1
HbA1c, HbF, HbA1a,
standard/ HbA1b, LA1c, HbA0, 90/290+1 L530 x W515 xH482
Tosoh G11 60s/test √ 2000 tests 12 months 3 μL >800 <1%
variant HbC, HbD, HbE, HbS, (STAT) 37kg (90 samples)
HbA1
HbA1c, HbF, HbA1a,
HA- standard/ L530 x W530 xH530
ARKRAY HbA1b, LA1c, HbA0, 50/100/200 24s/test Í 2000 ～ 3000 tests 12 months 8 μL 900 ≤1%
8190V variant 41kg
HbC, HbD, HbE, HbS
HbA1c, HbF, HbA1a,
Lifotroni standard/ HbA1b, LA1c, HbA0, 50/110+1 L722 x W532xH729
H100 96s/test √ 1000 ～ 1500 tests 6 months 5 μL 20,000 <1%
c variant HbC, HbD, HbE, HbS, (STAT ） 55kg
HbA2
HbA1c, HbF, HbA1a,
Lifotroni standard/ HbA1b, LA1c, HbA0, L500 x W520xH580
H9 110+1 (STAT) 130s/test √ 1000 ～ 1500 tests 6 months 5 μL 4,000 <1%
c variant HbC, HbD, HbE, HbS, 51.7kg
HbA2
HbA1c, HbF, HbA1a,
MEDCON MQ2000 standard/ 800/1600/2400 L610 x W500xH410
HbA1b, LA1c, HbA0, 50 160s/test Í 6 months 10 μL 20,000 ≤2%
N PT variant tests 28kg
HbC, HbD, HbE, HbS
WONDFO P60 VS OTHER
BRAND
Bio-rad
D100
Tosoh
G8
Tosoh Long lifespan column
G11
Small amount of sample volumn required
Powerful storage capacity
ARKRAY
HA-8190V No need to change Eluent1/2 & column for
variant mode
LIFOTHRONIC Wondfo P60
H100
LIFOTHRONIC
H9 MEDCONN
MQ2000PT
THANK YOU