Scribd
Upload
53 views10 pages

Types and Preparation of Pooled Platelets

Pooled Platelets are prepared by combining 6 units of random donor platelets, preferably matched for ABO or Rh type, into one bag. The preparation methods include pooling buffy coats or using platelet-rich plasma, with specific guidelines for shelf life and platelet content. Standards for leukocyte reduction and storage duration are also outlined, ensuring quality and safety in transfusion practices.

Uploaded by

drsathishtm
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
53 views10 pages

Types and Preparation of Pooled Platelets

Pooled Platelets are prepared by combining 6 units of random donor platelets, preferably matched for ABO or Rh type, into one bag. The preparation methods include pooling buffy coats or using platelet-rich plasma, with specific guidelines for shelf life and platelet content. Standards for leukocyte reduction and storage duration are also outlined, ensuring quality and safety in transfusion practices.

Uploaded by

drsathishtm
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Pooled Platelets

Concentrate
Types of Pooled Platelets
1. Pooled Platelets: prepared by pooling of 6 units of random donor
platelet, preferably ABO or Rh type matched are pooled into one
bag of “Pooled Platelets” (D & C Act Amendment 2020)
2. Platelets, Recovered, Pooled (EDQM)
3. Platelets, Recovered, Pooled, Leukocyte-Depleted (EDQM)
4. Platelets, Recovered, Pooled, in Additive Solution (EDQM)
5. Platelets, Recovered, Pooled, Leukocyte-Depleted, in Additive
Solution (EDQM)
6. Platelets, Recovered, Pooled, Pathogen-Reduced (EDQM)
Preparation of Pooled Platelet
Concentrate (D&C Amendment
2020)
• Up to 6 units of random donor platelets, preferably ABO or Rh type matched are
pooled into one bag of “Pooled Platelet Concentrate”.
• The pooled platelets may be prepared:
I. by pooling buffy coats and then processed into one unit of pooled buffy coats— pooled
platelet concentrate.
II. Alternatively, pooling can be done after preparation of random donor platelets by platelet
rich plasma method or buffy coat method.
• For open system (using spikes for pooling), the shelf life of the pooled platelets will
be 6 hours,
• For closed system (using sterile connecting device) the expiry date will be that of
the platelet unit having the shortest expiry date.
• The platelet content in the pooled platelet concentrate should be ≥ 2 X 1011/unit.
Pooled Platelet Concentrate (AABB
Standards 33rd ed)
• Four to 6 units of platelets are typically pooled to create one therapeutic dose.
• Dose of 3 x 1011 platelets in approximately 300 ml plasma, should meet 75% pooled units.
• LR pooled platelets must have a residual leukocyte count of < 5 x 106 per
transfusable dose and pH of >6.2 should meet 95% 0f units
The Council of Europe (EDQM 22nd
ed)
Slichter et al. 2012; Larsson et al.
2005
• Storage of pooled whole blood-derived platelet concentrates is now
accepted and both in vitro and in vivo studies suggest that
component quality is maintained for at least 7 days
Wildt-Eggen et al. 2001
• Leucocyte-reduced buffy coat platelet pools can be prepared either
by filtration or by centrifugation
• Leucodepletion filters especially designed for platelet concentrates
remove more than 90% of leucocytes from pooled platelets or
platelet concentrates collected with cell separators with a loss of less
than 15% of platelets.

You might also like