XS INTERNAL TRAINING
Product Specialist
PT. SABA INDOMEDIKA
XS-800i
XS-1000i
XS - Series
Automated 5-diff hematology analyzer
XS-1000i w/ sampler
XS Series: Your Priority Choice
2 Models
XS-1000i
XS-800i
System Flexibility
XS-1000i has the optional sampler for walk away operation
XS-1000i w/ Sampler
XE-2100
Sysmex Hema Family XE-2100D
XT-2000i
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XS-800i f
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KX-21N
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Parameters
24 parameters + 2 additional (same as XT-1800i)
WBC, NEUT#, LYMPH#, MONO#, EO#, BASO#, NEUT
%, LYMPH%, MONO%, EO%, BASO%, RBC, HGB,
HCT, MCV, MCH,MCHC, RDW-SD, RDW-CV, PLT,
PDW, MPV, P-LCR, PCT, IG#, IG%
Scattergram: Diff
Histograms: WBC, RBC, PLT
2 Discrete Modes
CBC
CBC + Diff also available with capillary mode
IPU – New design concept
OS is Window XP
New design IPU screen
Ease of use
Adopt free database program- Microsoft Data Engine
(XE & XT use SYBASE)
Superior Software Functionalities
XT/ XE pro functions are now available as standard in
XS IPU software
Reagent inventory management
Customized print outs
Definable QC range for statistical data
Compact Size
XS-1000i is smaller than KX-21 and a little bigger than pocH-100i
Dimensions pocH-100i XS-1000i KX-21
Width (cm) 18.5 32.0 42.0
Height (cm) 35.0 40.3 48.0
Depth (cm) 46.0 41.3 35.5
Weight (kg) 14 24 28
Compact Size
also smaller than Pentra 60, Celltac E or Act 5 PD CP
Celltac E Act 5 PD CP
Compact Size
Size comparison XS-1000i with competitor (front view)
XS-1000i Pentra60 / ACT Diff CP MEK-7222/CD-Pearl
Compact Size
Size comparison XS-1000i with competitor (top view)
XS-1000i Pentra60 / ACT Diff CP MEK-7222/CD-Pearl
PC PC
Low sample volume
Specially designed piercer for XS-1000i to reduce dead volume
Pipette use in XS-800i
1mm
Only 20uL aspiration volume (WB)
11µL WBC count and differential (CBC or CBC+Diff)
4µL RBC/PLT count and HGB detection
Sample Volume
Analyzer Mode Required Sample Volume Aspirated
sample Volume
13mm Ø microtube
XS-800i Manual open 500 µl 70 µl ~ 20 µl
Capillary 140 µl
- ~ 67 µl
(1:7 dilution) (20µl WB in 120µl Cellpack)
Manual closed 500 µl 90 µl ~ 20 µl
XS-1000i
Sampler > 1 ml Not available ~ 20 µl
Capillary 140 µl
(1:7 dilution) - (20µl WB in 120µl Cellpack) ~ 67 µl
Blood aspiration in XS-1000i
3 Adapters are provided for different sample tubes
For standard sample tubes
For micro sample tubes
For control blood - eCheck (XS)
Cap-piercing sampling for biohazard safety
Blood sensors monitor correct aspiration
Speed of the piercer needle is reduced.
The needle will always touch the bottom of the tube to
ensure the lowest possible blood volume needed for correct
measurement
No sample mixing function
Mixing is necessary prior to loading of sample
Closed Vial Sampler unit
Available for XS-1000i only
Optional item
Sampler capacity – 2 racks (20 samples)
No rack shifting inside the sampler
Integrated mixing and cap-piercing
Built in barcode reader
Sample tube rotating in front of the bar code reader to prevent
read errors.
STAT function available, open tube can be used
Physical start button
Throughput: 20 samples/ 23 minutes
Analysis modes
Analysis mode XS-1000i XS-800i
Manual Closed mode
Manual open mode
Capillary mode
Sampler mode
XS-800i
XS-1000i
XS-1000i w/ sampler
Throughput
Throughput
Analyzer Mode
(CBC, CBC+Diff)
XS-800i
Manual 60 samples/hr
Capillary 55 samples/hr
XS-1000i Manual 60 samples/hr
Capillary 49 samples/hr
Sampler 20 samples/23 min
Same Reagent System as XT & XE Series
4DL (5L or 2L) Cellclean
Cellpack
4DS (3x42ml)
Sulfolyser (3x 500ml)
Reagent Information
Product code Product Name Packing Open box
Stability
P88408711 Cellpack (PK-30L) 1X 20L 60 days
05433212 Stromatolyser-4DL (FFD-220A) 1 X 2L 60 days
P98417615 Stromatolyser-4DL (FFD-200A) 1 X 5L 60 days
P98417216 Stromatolyser-4DS (FFS-800A) 3x 42 ml 60 days
P90411317 Sulfolyser (SLS-210A) 3x 500 ml 60 days
83401621 Cell Clean 1X 50 ml -
05438619 eCheck, Level 1 4 x 1.5 ml 14 days
05438511 eCheck, Level 2 4 x 1.5 ml 14 days
05438716 eCheck, Level 3 4 x 1.5 ml 14 days
Reagent Consumption
Rinse / mth or
resume fr sleep*
CBC CBC+DIFF start-up shutdown auto rinse every1,200
(XS-800i/1000i)
samples
CELLPACK 32.0 32.0 162.0 17.0 72.0 74 / 72 94.00
STROMATOLYSER-4DL 2 2 4 0 4 4 0
STROMATOLYSER-4DS 0 0.03 0.06 0 0.06 0.06 0
SULFOLYSER 0.5 0.5 1 0 1 1 10
CELLCLEAN 0.0 0.0 0.0 0.0 0.0 0.0 3.0
* if no analysis is conducted for past 12 hrs, an auto rinse is carried
out when the system resumes from sleep
** FFS must be replaced every 1,200 analysis
Monthly rinse every monthly or after 1,200 samples
Reagent Comparison: XS vs XT
Unit : ml
CBC CBC + DIFF
Reagent
XS Series XT-1800i XS Series XT-1800i
Cellpack 32 31 32 33
Stromatolyser-4DL 2 0 2 2
Stromatolyser-4DS 0 0 0.03 0.04
Stromatolyser-FB 0 2 0 2
Sulfolyser 0.5 0.5 0.5 0.5
Comprehensive Control Function
X-bar / L-J control
20 QC files with 300 pts/file,
28 parameters
X-barM
1 X-barM file with 300 pts/file,
26 parameters
External Quality Control
SNCS on-line QC
(under development)
Control Material
eCheck (XS)
3 levels (L, N, H)
4 vialx 1.5 ml per box
open vial stability is 14 days
Assay values input from CD-
Rom
SNCS – on line qc service
Calibration
SCS-1000
Sysmex Recommendations
During Installation
Change of major parts
Indicated by QC data
Or any frequency required by regulatory or accreditation
bodies
DO NOT USE eCHECK for Calibration!
Data Storage
10,000 samples (incl. graphics)
5,000 patients’ information
1,000 selective test orders
Data Management System
HCLab Easy Access
Professional Patient Reports
Integrated Quality Control
Statistical Reports
Minimum Maintenance
Shut down
1x / day
Automated shutdown: 3 mins
Cell clean NOT Required
Monthly Maintenance
Every month or / after every 1,200 analyses
Rinse process: 15 mins
3 ml cell clean
Wash contaminants from optical detector block flowcell
Minimum Maintenance
As-needed maintenance
Auto Rinse
Waste chamber rinsing
3 ml cell clean
Remove Air Bubbles
Rinse Flowcell
3 ml cell clean
Drain Reaction Chamber
Drain RBC Isolation Chamber
Remove Clog
RBC Detector Aperture Cleaning
Cleaning the aspiration unit tray
Measurement Flow
WBC chamber
FFD 1 mL
5DIFF sample
FFS 30 uL
FCM detector
11uL
RBC/HGB chamber
RBC sample EPK （ 2mL ）
4uL
RBC
RBC
detector
Remained sample （ 1mL ） PLT
20uL whole blood （ 1mL ）
SLS （ 0.5m
L）
HGB detector HGB sample
Fluid System (new CBC mode)
Inside view from right side FCM
piercer
HGB WBC chamber
detector
RBC detector
RBC/HGB chamber
Technology
WBC and WBC + DIFF
Fluorescence Flow cytometery using semi conductor laser
(wavelength = 633 nm)
Stain: Polymethine Dye
Basis for fluorescence optical detection
Polymethine dye stains DNA/RNA
Fluorescence intensity is proportional to DNA/ RNA content in cell
RBC / PLT
Hydrodynamic focusing DC Detection Method
HGB
SLS method
Fluorescence Flow Cytometry
(Fluorescence Light)
(Side Scattered Light)
(Forward Scattered Light)
WBC classification
Aspirated whole blood
4ul 20ul 11ul
RBC chamber WBC chamber
RBC detector
Diluted Lyse (FFD)
Selected
discrete mode
Lyse (SLS)
FCM detector
Dye
(FFS) CBC CBC+ DIFF
HGB detector
WBC Classification
CBC + DiFF:
Same as in XE and XT
Stromatolyser-4DL and 4DS are used
Fluorescence + Side scatter signal are
used
New:
WBC# taken from DIFF scattergram
WBC #
WBC Classification
WBC #
NEW
Only stromatolyzer-4DL is used
Forward scatter is used for WBC# and
to provide a new histogram
Hydrodynamic Focusing
Central stream for measurement of Erythrocytes,
Thrombocytes and Hematocrit
Sheath flow in vertical direction:
Cellpack as sheath flow
The sample flow is drastically
accelerated due to the front sheath,
thus cells are separated and
approach the aperture one by one.
SLS-Hb Method
1. Hemolysis of RBC
2. Alteration of globulin
molecule
confirmation
3. Oxidation of Fe
4. Formation of SLS-
Hb
Normal
RBC Indices Ranges:
Mean Cellular Volume (MCV)
85 – 95 fl
HCT (%)
MCV (fl) =
RBC (x 106/µl)
Mean Cellular Haemoglobin (MCH)
HGB (g/dl) 28 – 32 pg
MCH (pg) =
1,68 – 2,05 fmol
RBC (x 106/µl)
Mean Cellular Hemoglobin
Concentration (MCHC)
HGB (g/dl) 32 – 35 g/dl
MCHC (g/dl) = 19,9 – 22,4
HCT (%)
mmol
RBC / PLT Histograms
The size distribution curve are separated by a moving threshold.
RL RU RBC
100 %
Erythrocytes have a size from 80-100 fl
and thus are measured between 25 and
250 fl.
20 %
100 200 250 fl
12 fl PLT
PL PU
100 % Platelets with a size from 8-12 fl are counted
between 2 and 30 fl.
A fixed discriminator is located at 12 fl.
20 %
Remark:The volume distribution curve has to
10 fl 20 fl 30 fl 40 fl start and finish at the base line between the
threshold lines.
Confidential Information – Internal use only!
Histogram - Distribution width
RDW-CV +/- 1SD
equivalent to 68.26 % of the distribution area
100 %
L2 - L1
RDW-CV (%) = X 100
L2 + L1
20 %
Normal Range 11 - 16 %
200 250 fl
L1 L2
inflection points of curve
RDW-SD
100 %
20 % Normal Range 37 - 46 fl
200 250 fl
intercept points of the curve at 20%
Confidential Information – Internal use only!
PLT-Parameters
PLT-Parameters
• PLT = Platelet Count
• MPV = Mean Platelet Volume (normal range: 8 – 12 fl)
• P-LCR = Platelet Large Cell Ratio (normal range: 12 – 35 %)
(fixed discriminator at 12 fl)
• PDW = Platelet Distribution Width (normal range: 9 – 14 fl)
• Measurement takes place between 2 and 30 fl.
PLT
PL 12 fl PU
100 %
PL:
P-LCR Flexible discriminator bet 2 and 6
fl.
PU:
20 %
Flexible discriminator bet 12 and
10 fl 20 fl 30 fl 40 fl
30 fl.
PDW
ANY QUESTIONS?
Thank you