ANTIBIOTIC
SENSITIVITY TEST
SAMEER QURESHI
BSC BIOTECHNOLOGY 3RD
YEAR
H.P.I.H.E
What is AST
A test done to check the effectiveness
of a drug against bacterium and to
select the best drug that act against
the bacterium.
REQUIREMENT
Nutrient Agar Media, Bacteria (KP),
Antibiotics: Septex‚ Ofloxacine, and
Tetracycline, Disk, Forceps, Pipette,
Pipettes tips, Glass spreader, Spirit
lamp.
PROCEDURE
60 ml nutrient agar media and 3
petri plates were prepared and
autoclaved at 121 °C for
15 min at 15 psi.
Media was poured to the plates and
left for solidification.
20µl of bacteria was spread with
spreader.
3 wells were punctured and
The 45µl medicine ( ofloxacine,
tetracycline and septex ) were
loaded in each well and Disk place
on medium .
Incubated at 37°C for overnight.
RESUL
T
Ceptex
Ofloxacin
disk
Tetracycline
Fig: Antibiotic sensitivity
test
S. No. Antibiot Diamete Diamete Mean
ics r of r of Diamete
zone of zone of r of
inhibitio inhibitio zone of
n on X- n on Y- inhibitio
axis (in axis (in n (in
mm) mm) mm)
1 Ofloxacin
32 34 33
2 Tetracyclin
e 14.5 14.5 14.5
3 Ceptex
22 22 22
4 Disk
Zone of inhibition =
Ofloxacin = = 33 mm
Tetracycline = = 14.5 mm
Ceptex = = 22 mm
Disk = = 9 mm
PRECAUTION
1. We always place the antibiotics
namely Ofloxacine, Tetracycline,
Ceptex and Disk in the wells in this
order after spreading of bacterial
inoculums on the petri plate and
before the bacterial colony have
grown. 2. Gloves
should be worn while spreading of
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