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Molecular Biology and Bioinformatics Overview

The document outlines a presentation on molecular biology, covering topics such as molecular techniques, bioinformatics, genomics, and proteomics. It discusses the structure of proteins, DNA sequencing, and various databases for nucleotide and protein sequences. Additionally, it highlights the importance of software tools for sequence analysis and primer design.

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Abdelrahim Mohammed
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6 views43 pages

Molecular Biology and Bioinformatics Overview

The document outlines a presentation on molecular biology, covering topics such as molecular techniques, bioinformatics, genomics, and proteomics. It discusses the structure of proteins, DNA sequencing, and various databases for nucleotide and protein sequences. Additionally, it highlights the importance of software tools for sequence analysis and primer design.

Uploaded by

Abdelrahim Mohammed
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Sofia bashir mohamed

Tropical medicine research institute


Out line of
presentations:
 Introduction to molecular biology.
 Molecular technique.
 Introduction to bioinformatics.
 Data base
 Software and tools
 Genomics
 Primer Design
 Proteomics
 What is molecular biology
Gene Structure
PROTIEN
Proteins are made up of polypeptide chains,
which are amino acids joined together with
peptide bonds
Hydrophilic
Hydrophobi
c
MOTIFES

DOMIN
S
Single nucleotide polymorphism
 Molecular technique.
DNA Sequencing:
Type of data

 Nucleotide sequences
 Protein sequences
 Proteins sequence of motifs and dominos.
 Macromolecular 3D structure
What is a data base
A structured collection: of data held in
computer storage; esp. one that
incorporates software to make it accessible
in a variety of ways; transf., any large
collection of information.
database management: the organization
and manipulation of data in a database.
database management system
(DBMS): a software package that
provides all the functions required for
database management. Primary
databases: experimental results directly
Nucleotide and protein sequence database

 Nucleotide Databases:
 EMBL( European Molecular Biology
Laboratory).
 GenBank.
 DDBJ–DNA Data Bank of Japan.
 Protein Databases:
 SWISS-PROT, PIR (sequences).
 PROSITE, PRINTS(sequence motifs.)
 HSSP, FSSP(classification, alignment).
 PDB (3-D structure).
Software Tools for Sequence Analysis
What might we want
to know about a Nucleotide sequence?

 Is this sequence similar to any known genes? How close


is the best match? Significance?

 What do we know about that gene?


 Genomic (chromosomal location, allelic information, etc.)

 Evolutionary information:
 Is this gene found in other organisms?

 What is its taxonomic tree?


 Recognition sites might be common:
 restriction enzymes, start sequences, stop sequences, other
regulatory sequences.
 Types of Mutations
 - Insertions
 - Deletions
 - Substitutions'
PRIMER DISEGIN
What might we want
to know about a proteins sequence?

 Conserved regions
 Structural motif

 Functional domains

 Homologous proteins

 Evolutionary common origin


 Structural similarity

 Functional similarity
Any Quotations?

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