Processes of Genetic Engineering
General Biology 2 - Quarter 3, Week
1A
Grade 11 STEM
Designed by [Your Name]
Date: [Insert Date]
Objectives
• - Define genetic engineering and its
importance.
• - Describe the processes involved in genetic
engineering.
• - Identify applications of genetic engineering
in various fields.
Introduction to Genetic
Engineering
• - Genetic engineering involves modifying the
DNA of an organism to change its
characteristics.
• - Purpose: Improve traits, produce specific
proteins, or study genes.
• - Examples:
• - Genetically Modified Organisms (GMOs)
• - Insulin production
Steps in Genetic Engineering
• 1. Isolation of the Gene of Interest: Locate and
extract the target gene.
• 2. Gene Cloning: Insert the gene into a vector
(e.g., plasmid).
• 3. Gene Transfer: Introduce the vector into the
host organism.
• 4. Expression of the Gene: Ensure the host
organism produces the desired
protein/product.
• 5. Screening and Selection: Identify successful
Tools in Genetic Engineering
• - Restriction Enzymes: Cut DNA at specific
sequences.
• - Ligases: Join DNA fragments.
• - Vectors: Carriers like plasmids and viruses.
• - PCR (Polymerase Chain Reaction): Amplify
DNA.
• - CRISPR-Cas9: A modern tool for precise
editing.
Applications of Genetic
Engineering
• - Medicine:
• - Production of insulin and vaccines.
• - Gene therapy for genetic disorders.
• - Agriculture:
• - Pest-resistant crops (e.g., Bt corn).
• - Drought-resistant plants.
• - Environment:
• - Bioremediation (cleaning up oil spills).
• - Reducing carbon emissions.
Ethical Considerations
• - Concerns:
• - Safety of GMOs for human health and the
environment.
• - Unintended consequences.
• - Ethics in Research:
• - Balancing innovation with responsibility.
• - Respect for biodiversity.
Summary
• - Genetic engineering is a powerful tool to
alter the DNA of organisms.
• - It involves processes like gene isolation,
cloning, transfer, and expression.
• - Applications are wide-ranging but must be
approached with ethical consideration.
Activity/Assignment
• **Task:**
• - Research and create a diagram of one
genetic engineering process.
• - Write a short paragraph explaining its
significance in real-world applications.
Thank You
Unlocking the mysteries of DNA for a
better future!