Lab orientation, magnification and surface to volume ratio in animals
BIO2135 Animal Form and Function Universit dOttawa / University of Ottawa
Todays objectives
Logging on and off the computers BIOLabo
Setting up bright field alignment (Compound microscope) Setting up the stereoscope
Image preview and capture (Image J software) Magnification
Fields of view
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Objectives (Cont.)
Understand factors affecting diffusion rates. Understand how surface to volume ratio is affected by the size of the animal Present quantitative data with a graph and caption Understand the role of data transformation, display error in measurements Gather and report the information
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BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Administrative information
Contact information Care and proper storage of microscopes
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Science network
Digital
Diversity open your copy Applications Launcher
BIOLabo Digital Zoology for when needed BIO2135 Website ImageJ
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Magnification
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Magnification specimens
Measurement specimens
Taenia pisiformis Amphioxus Blepharisma Aurelia ephyra Crayfish Planaria Liver fluke (Fasciola hepatica) Paramecium
Tetrahymena Spirostomum Planaria Liver fluke (Fasciola hepatica) Earthworm Sea urchin Perch Turtle Yourself 7
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Saving a Picture: Dos and Donts
Save file to K: drive in folder for Bio2135A2 My Documents on [Link]\homes\Undergrads\bio312d4(K:)
Save only as .jpg
Other file formats will not be transferred
DONT create subfolders in the Bio2135A2 folder
e.g. contents Bio2135A2\Lab 1 will NOT be transferred!
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Measuring surface to volume ratios
All measurements in mm!
Data recorded in spreadsheet on the designated computers BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Microscopy measurement
Snap and save your picture using image J. Open ruler in imageJ. Be sure both are at the same zoom.
40x
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
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Thought provoking questions for discussion
What things are moved (exchanged) across the surfaces of organisms? Where are the gas, nitrogenous waste, and nutrient exchange surfaces in each organism? Gas exchange requires a moist permeable surface how do organisms balance this requirement with water loss and how does where they live affect this? What prevents stagnation at the exchange surface? Are there ways to increase the concentration of the material being exchanged either inside or outside the animal? 11
BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Timeline
Dr. Housemans Prelab (10 min) Administrative Prelab (15 min) TA Prelab (15 min) Biolabo and magnification (50 min) Surface to volume (30 min) Group discussion (10 min) Class discussion (30 min)
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BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Graph Assignment using class data
(You plot all individual data points each student submits a points report, this is not a team assignment)
Graph #1- Surface Area as a function of volume Graph #2 - Log10 (Surface Area) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusion. Graph #3 - Log10 (Surface Area:Volume) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusions.
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BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa
Report
Data and instructions will be provided January 23, 2012 on the Bio2135 web site (Excel and pdf files). Due date will be January 30, 2012 before 4:00 pm in the document drop-off room box for your lab section.
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BIO2135 Animal Form and Function
Universit dOttawa University of Ottawa Universit dOttawa // University of Ottawa