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SEM and TEM Analysis of Nanoparticles

This document provides an overview of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphological evaluation of nanoparticles. It discusses the principles, parts, advantages, disadvantages and applications of SEM and TEM. It also describes the sample preparation process for SEM, which involves collection and cutting, fixation, dehydration, mounting, and sometimes coating of non-conductive samples. TEM sample preparation is not described. The document is intended as an introduction to using SEM and TEM techniques for nanoparticle characterization.

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wahid hussain
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63 views23 pages

SEM and TEM Analysis of Nanoparticles

This document provides an overview of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphological evaluation of nanoparticles. It discusses the principles, parts, advantages, disadvantages and applications of SEM and TEM. It also describes the sample preparation process for SEM, which involves collection and cutting, fixation, dehydration, mounting, and sometimes coating of non-conductive samples. TEM sample preparation is not described. The document is intended as an introduction to using SEM and TEM techniques for nanoparticle characterization.

Uploaded by

wahid hussain
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Morphological evaluation of Nanoparticle through

SEM & TEM Microscopy

Wahid Hussain
MPhil
pharmaceutics (2nd)
02332311013
Content of presentation:

• General introduction
• Principle, working and sample preparation of SEM
• Parts of SEM
• Advantages and disadvantages SEM
• Application of SEM
• Principle, working and sample preparation of TEM
• Parts of TEM
• Advantages and disadvantages TEM
• Application of TEM

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5
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SEM sample preparation:
1) Sample Collection and Cutting: 5) Mounting:
Collect the sample of interest and Cut or Attach the sample to an SEM stub or
section the sample to the desired size or specimen holder using conductive
shape, making sure it fits on the SEM stub
(a small metal or conductive mount). adhesives or double-sided carbon
2) Fixation: tape. Ensure good electrical contact
fixation is often required to preserve the to avoid charging effects.
sample's structure and prevent 6) Coating:
degradation. Common fixatives include
glutaraldehyde and formaldehyde. Non-conductive samples may need
3) Dehydration : to be coated with a thin layer of
for biological samples, remove water conductive material (e.g., gold,
from the specimen using a series of palladium, or carbon) to improve
ethanol or acetone washes. This is done
to prevent water-induced charging and their conductivity and prevent
artifacts during SEM imaging. charging. This is particularly
4) Critical Point Drying:
For some delicate samples, critical point
important for insulating
drying can be used to replace the water materials.
with a non-polar solvent like liquid
carbon dioxide. This prevents the collapse
of delicate structures during drying. 16
21

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