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Types and Uses of Microscopes

The document discusses different types of microscopes and their functions. It describes compound microscopes which use two or more lenses to magnify objects. Stereoscopic microscopes provide three-dimensional views of specimens. Electron microscopes use electron beams instead of light to achieve higher magnifications. Scanning electron microscopes produce 3D surface images while transmission electron microscopes allow observation of internal cell structures. Confocal laser scanning microscopes use lasers to generate 3D images of thick specimens.

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17 views23 pages

Types and Uses of Microscopes

The document discusses different types of microscopes and their functions. It describes compound microscopes which use two or more lenses to magnify objects. Stereoscopic microscopes provide three-dimensional views of specimens. Electron microscopes use electron beams instead of light to achieve higher magnifications. Scanning electron microscopes produce 3D surface images while transmission electron microscopes allow observation of internal cell structures. Confocal laser scanning microscopes use lasers to generate 3D images of thick specimens.

Uploaded by

harje panisa
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd

Microscopes

Objectives:
• Identify parts of the microscope and their functions
• Focus specimens using the compound microscope
• Appreciate that even microscopic organism/ materials
matters
• Employ appropriate techniques using the camera of their
smart phones to gather data about very small objects
STEREOSCOPIC MICROSCOPE
ELECTRON MICROSCOPE
COMPOUND MICROSCOPE
CONFOCAL LASER SCANNING
MICROSCOPE
Microscopy - an introduction
• Microscopes are instruments
designed to produce magnified
visual or photographic images of
small objects.

The microscope must accomplish three tasks


1. produce a magnified image of the specimen
2. separate the details in the image,
3. render the details visible to the human eye or camera.
• Microscopes increase
resolution - minimum
distance two points
can be apart and still
be distinguished as
two separate points

Topics
Microscope
One or more lenses that
make an enlarged
image of an object.

Topics
Simple Microscope
• Light passes through only 1 lens.
• Example: magnifying glass

Topics
Compound Microscope
• Lets light pass through an object and then
through two or more lenses.

Topics
Stereoscopic Microscope
• Gives a three dimensional view of an object. (Examples:
insects and leaves)
• Used for dissections

Topics
Electron microscopes – use a beam of electrons
instead of a beam of light to magnify the image

Topics
Electron Microscopes
• can achieve 3D images using electrons
The Scanning Electron Microscope
• produces a 3-dimensional image of
specimen’s surface features

spider head of a butterfly


Scanning electron microscopy (SEM)
Types of specimens:
-Whole organisms
-Natural tissue surfaces
-Exposed tissue structure

A flea magnified 50 000 X

What is this?
Scanning
Electron
Microscope

Topics
Transmission electron
microscopy (TEM).
• Allows the observation of molecules within
cells
• Allows the magnification of objects in the
order of 100, 000’s.
Transmission electron microscope (TEM)

– Provides for detailed study of the internal


ultrastructure of cells
– a beam of electrons Longitudina Cross
l section 1
is transmitted through section of
cilium
of cilium µm

the specimen for


a 2D view

Figure 6.4 (b) cilia on rabbit lungs


Confocal Laser Scanning Microscope (CLSM)

• laser beam used to


illuminate spots on
specimen
• computer compiles
images created from
each point to generate
a 3-dimensional image
• used on specimens
that are too thick for a
light microscope
Topics
A, B, C pollen grains: Scanning electron microscope
D pollen grains: Confocal Laser Scanning Microscope
E pollen grains: Transmission electron microscope
F pollen grains: Light microscope
G Mixed pollen grains (bright field light microscope, stained) H pollen grains
confocal laser scanning microscope

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