Scribd
Upload
65 views7 pages

Working Principles of Spectrophotometers

A spectrophotometer measures the amount of light absorbed by a sample. It works by passing light through a sample in a cuvette and measuring how much light is absorbed, based on the Beer-Lambert law which states that absorbance is linearly proportional to concentration. A basic spectrophotometer has a light source, monochromator to select wavelengths, sample holder, and detector. It is commonly used in the food industry to measure properties like moisture, fat, protein, and carbohydrates simultaneously and quickly.

Uploaded by

Shagun
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
65 views7 pages

Working Principles of Spectrophotometers

A spectrophotometer measures the amount of light absorbed by a sample. It works by passing light through a sample in a cuvette and measuring how much light is absorbed, based on the Beer-Lambert law which states that absorbance is linearly proportional to concentration. A basic spectrophotometer has a light source, monochromator to select wavelengths, sample holder, and detector. It is commonly used in the food industry to measure properties like moisture, fat, protein, and carbohydrates simultaneously and quickly.

Uploaded by

Shagun
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

WORKING OF

SPECTROPHOTOMETER

Submitted by- Shagun Rana


Submitted to- Dr. Prince Chawla
WHAT IS SPECTROPHOTOMETER?

• A spectrophotometer is an instrument that measures the amount of light absorbed


by a sample.

• Spectrophotometer techniques are mostly used to measure the concentration of


solutes in solution by measuring the amount of the light that is absorbed by the
solution in a cuvette placed in the spectrophotometer.

• Scientist Arnold J. Beckman and his colleagues at the National Technologies


Laboratory (NTL) invented the Beckman DU spectrophotometer in 1940.
PRINCIPLE

• Spectrophotometry is a scientific method based on the absorption of


light by a substance, and takes advantage of two laws of light
absorption.
• Beer-Lambert Law (also known as Beer's Law) states that there is a
linear relationship between the absorbance and the concentration of a
sample.
WORKING OF
SPECTROPHOTOMETER

There are four basic components to a simple single beam


UV/Vis spectrophotometer; a light source, a monochromator, a
sample, and a detector.
APPLICATIONS IN FOOD INDUSTRY

• The spectroscopy has been applied to measure moisture, fat,


protein and carbohydrate content in wide variety of foods.

• The most significant advantage is its ability to determine


simultaneously several components in a food sample within a short
time.
CONCLUSION

• Spectrophotometer is an instrumental technique, which is used


to measure the reflectance or transmittance light of the object
across the full spectrum of visible light wavelengths, 400 nm to
700 nm.

• The Spectrophotometer is working on the basis Beer-Lambert’s


law, it offer greater specificity, making them the instruments of
choice for food product color formulation, specification of
standards and tolerances, inter-plant color communication and
color quality control in processing operations.

You might also like