Scribd
Upload
156 views17 pages

Parts and Functions of a Compound Microscope

The document describes the parts and proper use of a compound microscope. It has magnifying parts like objectives and eyepiece that make specimens appear larger. It uses illuminating parts like a mirror, condenser, and diaphragm to direct light onto specimens. Mechanical parts like the stage, nosepiece, and adjustment knobs are used to hold slides and focus images. Specimens are mounted on slides under coverslips and viewed through objectives at various magnifications. Proper handling and slide preparation are important for clear viewing.

Uploaded by

Jamil Samira E. Buizon
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
156 views17 pages

Parts and Functions of a Compound Microscope

The document describes the parts and proper use of a compound microscope. It has magnifying parts like objectives and eyepiece that make specimens appear larger. It uses illuminating parts like a mirror, condenser, and diaphragm to direct light onto specimens. Mechanical parts like the stage, nosepiece, and adjustment knobs are used to hold slides and focus images. Specimens are mounted on slides under coverslips and viewed through objectives at various magnifications. Proper handling and slide preparation are important for clear viewing.

Uploaded by

Jamil Samira E. Buizon
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Compound Microscope

Parts of the Microscope

1
Magnifying Parts
Ocular Lens/Eyepiece
contains the magnifying lens you
look through

Oil Immersion Objective


contains the highest magnification
power of 100x with the aid of a
specialized oil.

High Power Objective


Contains a magnification power
of 40x

Low Power Objective


Contains the least magnification
power of 10x
2
Illuminating Parts
Condenser and Diaphragm

Mirror
Reflects the light towards the
condenser. Has a concave and flat
sides.

3
Illuminating Parts
Condenser
is used to collect and focus the
light from the illuminator on to
the specimen

Diaphragm
controls the amount of light
reaching the specimen
4
Diaphragm

5
Mechanical Parts
Body Tube
connects and eyepiece and the
revolving nosepiece.
Revolving Nosepiece
holds and turns the objective lenses
into viewing position.

Stage
supports the glass slide that contains
a specimen

Stage Clip
holds the glass slide in place.

6
Mechanical Parts
Coarse Adjustment Knob
focuses the image under low power

Fine Adjustment Knob


sharpens the image under low and
high power

Arm
supports the body tube

Inclination Joint
allows the tilting of the microscope

Base
supports the microscope
7
Field of View
it is the lighted area
where the magnified
part/s of the specimen is
seen.
always ensure that its
properly lighted by
adjusting the
mirror and the
diaphragm
8
Total Magnification

9
Proper Handling and Usage

 Carry the microscope by holding the arm in


one hand and the base on the other hand.
10
Proper Handling and Usage

 Do not let the lens hit the slide.


11
Proper Handling and Usage

 Always look at the eyepiece with both eyes


open.
12
Proper Handling and Usage
 Do not let the direct
sunlight strike the
microscope mirror
 Never use the

coarse
adjustment knob
when the HPO or
OIO is in position.
13
Slide, Cover Slip and Specimen
 The slide is where
the specimen is
mounted.
 Coverslip prevents
the specimen to
have direct contact
to the objective lens.

14
Slide, Cover Slip and Specimen

 Water is used to fix the specimen and


keep the cover slip in place.
15
Slide, Cover Slip and Specimen

 If the specimen is transparent a stain


is used to see its detail.
16
Slide, Cover Slip and Specimen
 Never place overflowing specimens.
Use a tissue to remove extra water or
stain.
 A specimen or sample is the object
that is being observed.
 Do not forcefully press the cover slip
against the specimen and the slide.
17

You might also like