Photochemical screening,
antimicrobial and antioxidant
properties
Tweneboah Koduah, Stephen
1. Study of the effect of UV light on
the antimicrobial activity of
Euphorbia hirta leaf extract
Methodology
The leaves of Euphorbia hirta was collected
↓
The leaves of the plant was washed thoroughly 2-3 times under running water
↓
It was once with sterile distilled water.
↓
Shade-dried and converted into small pieces by using mortar and pestle into
powdered form.
↓
The powdered plant was passed through sequential solvent extraction.
↓
Extraction
Hexane
↓ Ethanol
↓ ↓
↓
Chlorofor
m
↓
↓
↓
The isolated extract was evaporated using rotary evaporator
↓
Concentrated extracts were subjected to various chemical tests in order to
detect the various phytoconstituents.
↓
The powdered plant was passed through sequential solvent extraction.
2. Phytochemical and
antimicrobial study of the
seeds and leaves of Peganum
harmala L. against urinary
tract infection pathogens
Methodology
The seeds and leaves
of P. harmala
were collected
50g of dry
50g of dry 50g of dry 50g of dry
powder (leaves
powder (leaves powder (leaves powder (leaves
and seeds)
and seeds) and seeds) and seeds) +250
+250 mL of
+250 mL +250 mL of mL of methanol
methanol for 1
distilled water distilled for 1 for 1 hour
hour
for 1 hour hour
Filtered by using
Whatman filter paper.
The filtrate was used
for
Phytochemical
screening.
3. Screening of Zingiberaceae
extracts for antimicrobial and
antioxidant activities
Methodology:
The plant material (Zingiberaceae) were collected
↓
About 300 g of the rhizome and root parts of the plant were cut into smaller pieces and air-dried
under the shade
↓
Extraction
Dichloromethane Methanol
[3 times] [3 times]
↓ ↓
Extract was evaporated Extract was evaporated
under reduced pressure under reduced pressure
4. Phytochemical Analysis
and Antimicrobial Activity
of Leaf and Stem Extracts
of Priva cordifolia (L.f.)
Druce
Continuation:
• Preparation of Extracts
• Dried and weighed plant material powder of plant
material was soaked in 3 different solvents i.e.
ethanol, methanol and water and kept for 24-48 hrs
at 370C. The solvent extracts obtained were filtered
using Whatman filter paper number 42 and solvents
were removed under reduced pressure using
vacuum drying and air dried.
5. Study on phytochemical
antioxidant, antimicrobial analysis
and separation of bioactive leads of
leaf extract from selected mangroves.
Methodology
Mangroves species namely Suaeda nudiflora, Luminitzeva racemara,
Iponioca tuba and Avicennia alba were collected
↓
Washed with tap water and cut into small pieces and dried
under shade away from sunlight
↓
The dried leaves were made into powder and stored in polythene bags.
↓
10g of the powder plant material was mixed with 100ml methanol and stored
for 72hrs and then filtered
↓
The solvent was removed by rotary vacuum evaporation and
the extracts were used for analysis
6. Screening phytochemical
content,antioxidant,antimicrobial and
cytotoxic activitiesof catharanthus
raseus(L.) [Link]( [Link]) and its
fractions.
Methodology
[Link] was collected and identified
↓
It was cut into sections(2.00cm length) and frozen at 80oC in an ultra
low freezer.
↓
The dried samples were grounded and sieved to uniform
size(<1.40mm) and stored at 20oC until required.
7. Analysis of phytochemical
constituents, antibacterial,
antioxidant, photoprotective activities
and cytotoxic effect of leaves extracts
and fractions of Aloe Vera.
Methodology:
Fresh leaves of Aloe Vera were collected and washed under
running water to remove debris and then rinsed in distilled water,
thrashed into a fine powder using an electric blender and stored air
tight for extract preparation.
10g was extracted by 5g of leaf was mixed
maceration in 100ml with 100ml of acetone
of 70% for 72hrs
meoH, 80% for 24hrs,
3 extractions.
The filtrates were The filtrates was
combined and evaporated evaporated at 40oC by
at 40oC by rotary rotary evaporator.
evaporator. (Zhang et al(2008)
(Yanlum et al(2006)
8. Phytochemical screening and
antimicrobial activity of Olea
europaea subsg. Africana against
pathogenic microorganisms
Methodology
Fresh leaves of Olea africana were collected
↓
The leaves were washed and air dried at 25oC for 2 weeks
↓
Ground into powder and stored in plastic bags.
↓
100g of the powder was extracted with 500ml of each solvent(butanol, ethanol, ethyl
acetate, hexane, methanol and water) in a rotary shaker for 72hrs.
↓
The extracts were filtered through Whatman filter paper and freeze-dried in a lyophilizer
and the dried extracts were stored in 50ml centrifuge tubes maintained at 4oC for further
use.
↓
9. Determination of antimicrobial and
phytochemical compound of Jatropha
curcus plant
Methodology
Various parts of Jatropha curcus plant were collected
(root, seed, stem, leaf and flower)
↓
The different parts were washed with distilled water to avoid any
microbial growth and then put into polythene bags.
↓
Different parts of J. Curcus extracts were prepared in solvents and
water and left to soak at room temperature, (21-22 oC) for 24hrs.
↓
The extracts were strained through cheese cloth, centrifuges and stored
at 4oC to further experiment.
10. Phytochemicals, Mineral Contents,
antioxidants, antimicrobial, activities of
propolis produced by Brunei stingless
bees Geniotrigona thoracica,
Heterotrigona itama and Tetrigona
binghami.
Methodology
The propolis of three stingless bee species, [Link], H. itama
and T. binghami were collected
↓
Ethanolic extract of the propolis was obtained by macerating
The raw dried propolis in 96% ethanol.
↓
The suspension was crystated at 150npm at 37 oC for 18hrs and then
passed through vacuum filtration to remove particulates.
↓
The supernatant was rotary evaporated until approximately half solvent
volume reduction and then dried in an oven at 40 oC.