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RFLP vs RAPD: Techniques and Applications

RFLP and RAPD are genetic analysis techniques. RFLP involves cutting DNA with restriction enzymes, separating fragments via gel electrophoresis, and using probes to identify genotypes based on fragment size. It can be used for paternity testing and detecting known mutations. RAPD uses PCR and arbitrary primers to amplify polymorphic DNA regions without prior sequencing. It provides a quick screen for genetic variation and its major advantage is that it does not require pre-sequencing of DNA.

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25 views13 pages

RFLP vs RAPD: Techniques and Applications

RFLP and RAPD are genetic analysis techniques. RFLP involves cutting DNA with restriction enzymes, separating fragments via gel electrophoresis, and using probes to identify genotypes based on fragment size. It can be used for paternity testing and detecting known mutations. RAPD uses PCR and arbitrary primers to amplify polymorphic DNA regions without prior sequencing. It provides a quick screen for genetic variation and its major advantage is that it does not require pre-sequencing of DNA.

Uploaded by

saboor saifi
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd

RFLP AND RAPD

RFLP( Restriction fragment length


polymorphism)
• It is a genetic variant that can be examined by
a cleaving the DNA into fragments with a
restriction enzymes.
• RFLP TECHNIQUEES-
1. It involves following techniques.
2. DNA sequences are cut through restriction
enzymes
3. Then separated by gel electrophoresis
• DNA is transfered on to
nitrocellulose membrane& labelled
with probe sequences.
• Genotypic can be recognised by
altered restriction fragments
RFLP
Application of RFLP
• It can be used in paternity cases or criminal
cases to determine the source of a DNA sample.
• Determining the individual status of an
individual i.e. it can be used in the detection of
mutation particularly known mutation.
• To measure recombination rates which can
leads to a genetic map with the distance b/w
RFLP loci measured in centi morgan.
• RAPD ( Random
amplification polymorphic DNA)
Principle- it is a PCR BASED technique for
identifying the variation it involves the use of
single arbitory primer in a PCR reaction,
resulting in amplification of many discrete
DNA. IT provides a quick and efficient screen
for DNA sequenced based polymorphism at a
very large number of loci. The major
advantage of RAPD that it does require pre
sequencing of DNA.
• METHODS OF RAPD

Extraction of DNA.
Quality and quantity check of extracted DNA.
Selection of primers
PCR amplification.
Agarose gel electrophoresis of PCR amplified
DNA.
Statically analysis.
Advantages of RAPD
i. Suitability for work on anonymous genomes
ii. applicability to problems where only limited
quantities of DNA are available.
iii. Efficiency of low expense, high number of
fragments are formed.
Disadvantages of RAPD
RAPD markers are dominant amplification either
occurs at a locus or it does not , leading to
scores based on band presence or absence.
this means that homozygotes and
heterozygotes cannot be distinguished.
Application of RAPD
1. Genetic mapping.
2. Population & evolutionary genetics.
3. Plant & animal breeding.
4. Reproducibility of RAPD markers.
5. Developing genetic markers linked to a trait
in question.

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