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Microscope Usage and Components Guide

This document describes the parts and proper use of a microscope. It lists the major components of a microscope, including the eyepiece, stage, objectives, revolving nosepiece, and light source. It explains that a microscope is used to magnify small objects and see details not visible to the naked eye. The document provides instructions for setting up and focusing a microscope, such as using the coarse and fine adjustment knobs to bring the specimen into view. It also describes how the different objectives and eyepiece combine to provide different levels of total magnification.

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Genevieve Desiderio
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7 views18 pages

Microscope Usage and Components Guide

This document describes the parts and proper use of a microscope. It lists the major components of a microscope, including the eyepiece, stage, objectives, revolving nosepiece, and light source. It explains that a microscope is used to magnify small objects and see details not visible to the naked eye. The document provides instructions for setting up and focusing a microscope, such as using the coarse and fine adjustment knobs to bring the specimen into view. It also describes how the different objectives and eyepiece combine to provide different levels of total magnification.

Uploaded by

Genevieve Desiderio
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

MICROSCOPE

• Prepared by:
Ms. Marisol C. Marcelo, LPT
Microscope

 An instrument used to see


objects that are too small
to be seen by the naked
eye.
Rules of using a microscope
Always carry with 2 hands
Never touch the lenses with
your fingers.
Only use lens paper for cleaning
After using, rotate the
nosepiece to low power
objective, roll the stage down
to lowest level and rubber
band the cord,
Parts of the
Microscope and
Their Function
Parts of a microscope

Eyepiece
Where you look
through to see
the image of
your specimen.
Contains the
10X
magnifying
lens
Parts of a microscope
Coarse
Adjustment
Knob
Moves stage up
and
down
Focuses the
image under
low power
Use this first
Parts of a microscope
Fine
Adjustment
Knob
Focuses the
image under
high power
To fine-tune
the focus of
your specimen
after using
the coarse
adjustment
knob.
Parts of a microscope

Revolving
Nosepiece
Holds the
objective lenses
Revolves to
change
magnification
Parts of a microscope

Objectives
Low power
(shortest)
Magnifies 4X
Medium power
Magnifies 10X
High power
(longest)
Magnifies 40X
Parts of a microscope

Stage
Supports and
holds the
microscope
slide while
viewing
Parts of a microscope

Stage clips
Place on
microscope slide
to secure into
place after you
have focused on
your specimen
Parts of a microscope
Diaphragm
Controls
the
amount of
light
available
while
viewing
sample
Parts of a microscope

Light source
Shines light up
through the
diaphragm and
stage to light
the object
being viewed
Lighted area is
called your “Field
of View”
Parts of a microscope

Body tube
connects
the
eyepiece to
the
objective
lenses
Parts of a microscope

Arm
Supports the
body tube
which
connects the
eyepiece to
the set of
objective
lenses
Parts of a microscope

Base
Supports the
microscope
Total
Magnification
Multiply the eyepiece
magnification (10X) by the
objective magnification (4X,
10X, 40X)
Example: 4 x 10 = 40X total
How to use a microscope
•Place the slide on the stage
•Use stage clips to secure slide
•Adjust nosepiece to lowest setting
• (Lowest = shortest objective)
•Look into eyepiece
•Use coarse adjustment knob
•Use fine adjustment knob

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