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Forward and Reverse Blood Grouping Methods

1. The document discusses ABO blood grouping, which determines a person's blood type (A, B, AB, or O) through mixing red blood cells with specific antisera and observing agglutination. 2. It provides instructions and questions for exercises on forward and reverse ABO grouping. Forward grouping detects antigens on red blood cells, while reverse grouping identifies antibodies in serum/plasma. 3. Precautions for ABO grouping include using unexpired reagents, proper incubation times, and equal amounts of antisera and blood samples to avoid false positive or negative results.

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2K views11 pages

Forward and Reverse Blood Grouping Methods

1. The document discusses ABO blood grouping, which determines a person's blood type (A, B, AB, or O) through mixing red blood cells with specific antisera and observing agglutination. 2. It provides instructions and questions for exercises on forward and reverse ABO grouping. Forward grouping detects antigens on red blood cells, while reverse grouping identifies antibodies in serum/plasma. 3. Precautions for ABO grouping include using unexpired reagents, proper incubation times, and equal amounts of antisera and blood samples to avoid false positive or negative results.

Uploaded by

cyrhenmie
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
  • Introduction
  • Exercise 3: ABO Grouping (Forward)
  • Exercise 4: ABO Grouping (Reverse)
  • Reference

EX. 3: ABO GROUPING (FORWARD) EX.

4: ABO GROUPING (REVERSE)


BSMT 3B
Group 9: Bacani, Ronaline Anne L. Jocson, Jancy R. Ocampo, Karl A. Velasco, Princess Rochelle S.

INTRODUCTION
1900 karl landsteiner Test performed to determine an individual's blood type 4 main blood groups: A, B, O, or AB Important in transfusion medicine In transfusion medicine

EXERCISE 3: ABO GROUPING (FORWARD) GUIDE QUESTIONS:


1. List some advantages of the tube method over the slide method of ABO grouping.

It allows for fairly long incubation Centrifugation Simplicity of reading and grading of results. Clean and more hygienic. Requires smaller volume of reagents.

EXERCISE 3: ABO GROUPING (FORWARD) GUIDE QUESTIONS:


2. Restate the principle of ABO forwarding typing. Antigens present on RBCs are detected by mixing the red cells with specific anti-sera and observing agglutination, either after centrifugation (tube method) or after standing at room temperature (slide method). Agglutination is a positive reaction

EXERCISE 3: ABO GROUPING (FORWARD) POST-LAB CONFERENCE QUESTIONS:


1. List some precautions in ABO grouping and explain briefly.
Expired reagents Proper incubation Equal amount of anti-sera and the blood sample

EXERCISE 3: ABO GROUPING (FORWARD) POST-LAB CONFERENCE QUESTIONS:


2. Why is it necessary to wait for two minutes before reading the result of the slide method?

In slide method, you must wait until two minutes to let the antigen to react to the anti-sera.

EXERCISE 4: ABO GROUPING (REVERSE) GUIDE QUESTIONS:


1. What is the importance of reverse grouping? Identifies the presence of antibodies Confirms the reaction obtained by the forward grouping test Tests the serum/plasma from the recipient or donor with group A red cells and group B red cell

EXERCISE 4: ABO GROUPING (REVERSE) GUIDE QUESTIONS:


2. What are the limitations of the reverse groupings?
False negative reactions False positive reactions

EXERCISE 4: ABO GROUPING (REVERSE) POST-LAB CONFERENCE QUESTIONS:


1. List some precautions in performing the reverse type. Explain each Use the recommended strength of cell suspension Do not warm the ABO reagent before performing the test. If an antibody seems to be missing or weak in the reverse typing, add 2 additional drops of patients serum and centrifuge, if still missing decrease the temperature for 10 minutes and centrifuge Use white background in reading the result

EXERCISE 4: ABO GROUPING (REVERSE) POST-LAB CONFERENCE QUESTIONS:


2. Discuss the difference between antiserum and patient ABO antibodies. Antiserum refers to the blood from an immunized host from which clotting proteins and red blood cells have been removed. An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens.

REFERENCE
Harmening, D.M. (2012). Modern Blood Banking & Transfusion Practices 6th Edition. F.A. Davis. [Link] [Link] [Link] [Link] [Link] [Link] _group_system.htm [Link]

EX. 3: ABO GROUPING (FORWARD) EX. 4: ABO GROUPING (REVERSE) BSMT 3B Group 9: Bacani, Ronaline Anne L. Jocson, Jancy R.
INTRODUCTION • 1900 karl landsteiner • Test performed to determine an individual's blood type • 4 main blood groups: A
EXERCISE 3: ABO GROUPING (FORWARD) GUIDE QUESTIONS: 1. List some advantages of the tube method over the slide method of
EXERCISE 3: ABO GROUPING (FORWARD) GUIDE QUESTIONS: 2. Restate the principle of ABO forwarding typing. • Antigens prese
EXERCISE 3: ABO GROUPING (FORWARD) POST-LAB CONFERENCE QUESTIONS: 1. List some precautions in ABO grouping and explain b
EXERCISE 3: ABO GROUPING (FORWARD) POST-LAB CONFERENCE QUESTIONS: 2. Why is it necessary to wait for two minutes before
EXERCISE 4: ABO GROUPING (REVERSE) GUIDE QUESTIONS: 1. What is the importance of reverse grouping? • Identifies the p
EXERCISE 4: ABO GROUPING (REVERSE) GUIDE QUESTIONS: 2. What are the limitations of the reverse groupings? • False neg
EXERCISE 4: ABO GROUPING (REVERSE) POST-LAB CONFERENCE QUESTIONS: 1. List some precautions in performing the reverse typ
EXERCISE 4: ABO GROUPING (REVERSE) POST-LAB CONFERENCE QUESTIONS: 2. Discuss the difference between antiserum and patien

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