Rapid Urease Test Broth M1828
Rapid Urease Test Broth is recommended for rapid detection of urease production.
Composition**
Ingredients Gms / Litre
Yeast extract 0.100
Urea 20.000
Monopotassium phosphate 0.091
Disodium phosphate 0.095
Phenol red 0.010
Final pH ( at 25°C) 6.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 20.30 grams in 1000 ml distilled water. Mix well and sterilize by filtration. DO NOT AUTOCLAVE OR HEAT THE
MEDIUM. Dispense in sterile tubes as desired.
Principle And Interpretation
In Rapid Urease test Broth the urease reaction given by H. pylori , occurs more quickly than that seen by other organisms
which may split urea. As a result, it is an effective presumptive test for the presence of H. pylori . It is also used for the rapid
detection of urease activity in bacteria such as Proteus spp., or in yeast, (such as Cryptococcus neoformans).
Helicobacter pylori is a gram negative, curved, microaerophilic and motile organism with multiple polar flagella.
Helicobacter pylori is a spiral urease producing organism that lies in the interface between gastric epithelial cell surface and
the overlying mucus gel (1). It resides in the stomach of man and other primates, lining up the gastric mucus secreting cells.
Rapid urease test is one of the invasive tests. This method has been used to help simplify the diagnosis of H. pylori, especially
those specimens originating from duodenal and gastric ulcers, and chronic antral gastritis (type B).
This medium is develop as per McFaddin (3). Urease activity can be described as the splitting of urea via hydrolysis by a
urease enzyme. The end products from this reaction yield ammonium carbonate and ammonia, which are alkaline in nature.
The consequent rise in the pH of the medium is detected by phenol red indicator. The test is non-toxic, and the pH change that
occurs from accumulation of alkaline end products is detected by a pH indicator in the media (2). Helicobacter pylori is an
organism that may be easily identified by this test because of its very high endogenous urease activity.
Yeast extract which provides nitogen and vitamin required for growth. Phosphates serve to buffer the medium.
Quality Control
Appearance
Light yellow to light pink homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellowish orange coloured clear solution in tubes.
Reaction
Reaction of basal medium (1.87gm in 100ml distilled water) at 25°C. pH : 6.8±0.2
pH
6.60-7.00
Cultural Response
M1828: Cultural characteristics observed after an incubation at 35-37°C for 4-18 hours.
Organism Inoculum Urease
(CFU)
Cultural Response
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Enterobacter aerogenes 50-100 negative
ATCC 13048 reaction, no
change
Escherichia coli ATCC 50-100 negative
25922 reaction, no
change
Klebsiella pneumoniae 50-100 weak positive
ATCC 13883 reaction
Proteus vulgaris ATCC 50-100 positive
13315 reaction, cerise
colour
Salmonella Typhimurium 50-100 negative
ATCC 14028 reaction, no
change
Helicobacter pylori ATCC 50-100 positive
43504 reaction, cerise
colour
Klebsiella pneumoniae 50-100 weak positive
ATCC 10031 reaction
Storage and Shelf Life
Store at 2-8°C in tightly capped container. Use before expiry date on the label.
Reference
[Link] MA,Pajares-Garcia Epidemiology and transmissin of Helicobacter pylori . Curr Opin Gasteroenterol 1995; 11(supp
1) : 1-4.
[Link] PD, Graham DY, Gaillour A et al water source as risk factor for Helicobacter pylori infection in Peruvian children.
Lancet 1991 ; 337 : 1503-06.
[Link], Jean F-Biochemical tests for identification of medical bacteria / Jean F. Macfaddin1980; 424. ,,
Revision : 2 / 2015
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User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
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