Microscope Digital Camera

DP74

Intelligent Imaging for an Improved Workflow

Not for clinical diagnostic use.

 Intuitive Workflow Enhancements for Easy Operation

The DP74 color fluorescence camera captures realistic, high-quality images and has features that enable users to make
their observations easily. With a wide field of view, operators can capture images of more of their sample, quickly.

In applications like histology, the DP74 camera faithfully reproduces colors to render natural images of your specimen.
The camera displays a realistic image, so what appears on the monitor looks the same as what users see looking through
the microscope’s eyepieces. Users can remain comfortable during their work since they can just watch the monitor rather
than having to go back and forth between the monitor and eyepieces. The camera is easy to use, so it integrates into any
workflow, making it simple to capture publication-quality images.

Enhanced features make the DP74 camera a powerful research tool. For applications like histology, where users need to
regularly capture images for daily discussion, the position navigator keeps track of your location, so you will always know
precisely where you are on your sample when making high-magnification observations. Optimized for fluorescence color
imaging on live specimens, the live noise reduction feature seamlessly assists with focusing on dim fluorescent images.

1
Fast, high sensitivity, and superior quality image
with the latest CMOS technology.

Superior
S uperior
Hig
High
H igh IImage
mage Q uality
Quality
Sensitivity
S
Sensit
ensitivity

Intelligent
Intelli
Intelligent
Smooth
S mooth Assist
Assist
Ass
Observation
Observation

Smart camera navigation for comfortable observation.

Observation Image

Overview

2
 Capture High-Quality Images Quickly and Easily

Full HD Images of Live Specimens are Fast and Simple to Acquire

Obtain high-quality images of live specimens higher than FullHD resolution (2.3 million native pixels). The DP74 camera delivers
images at a full 60 frames per second. The high frame rate enables users to make observations quickly and easily and capture
fast movements in the specimen. The DP74's global shutter eliminates the live image distortion frequently caused by rolling
shutter CMOSs.

DP74
(60 fps)

Smooth live images make it easy locate an object's position.

Conventional Live image is not updated Live image is not updated

(15 fps)

Because of the poor responsiveness of live images to the microscope's handling, the position of the object is not clear.

One of the Widest Field of Views Available

The DP74 color fluorescence camera features a field
of view that is 37% larger than conventional microscope
cameras, which are typically the equivalent of FN 18 or less. DP74 FOV
The wide 16:10 aspect ratio field of view enables users Diagonal
(FN 21 equivalent)
to find areas of interest more quickly and recognize their
position within a sample.
Conventional FOV
Diagonal
(FN 18 equivalent)

Binocular FOV
FN 22

High Color Fidelity for Most Stains Used in the Life Sciences
Olympus’ proprietary image processing technology delivers
excellent color reproducibility in a wide variety of stained
samples. The color fidelity detects subtle shading, faithfully
reproduces the tint from HE staining, light green from
Papanicolaou staining, and brown from DAB staining.

High Color Fidelity

3
Capture Fine Details at Low Magnifications
The DP74 camera provides high resolution at low
magnification. The camera’s pixel shifting technology
provides sub-pixel information, delivering the equivalent of
20.7 million pixels in a high-resolution image. The 3-CMOS
mode makes each pixel's information as accurate as three
chip cameras. This provides more detail, creating high- Without shift

definition images with fine color reproduction without

increasing the file size.

With shift

Vivid Fluorescence Images with Reduced Noise

Because the CMOS sensor is cooled, it enables sharp
fluorescence images with low noise and with sensitivity
similar to or greater than a cooled CCD. The camera’s
‘Linear’ mode provides accurate brightness data that is
proportional to the number of sensed photons, so it is
easier to analyze the brightness of fluorescence images.

Seamless Stitching with Even Illumination Across the Image

With the shading correction feature, it’s possible to produce
beautiful macro images with fewer seams. Tiling is faster
thanks to the wider field of view and the fast frame rate,
leading to beautiful macro images.

With shading DP74

(for illustration purpose only)

4
 Enhanced Efficiency and Comfort

Easily know the location of interest with the Position Navigator

The position navigator adds convenience to the operator’s workflow because it creates a map to help users keep track of their
location within the specimen; this is especially useful when making observations at high magnification. This is achieved without
the need for a motorized or encoded stage. The low-magnification map of the sample containing all of the pertinent location
information can be stored along with the high-magnification observation image, making it convenient for record keeping or
creating presentation materials.
*The accuracy of the map creation and the position navigation depends on observed samples.

Easily determine your current position Easily discuss your findings

Customers’ Survey with Observe with Discuss using high

Change obj. Capture
operation low power high power magnification image
DP74 +
Position Automatic map Display the current Save the image context map
navigator’s creation observed position with the map
function on the map

Conventional Customer Loses Their Position in the Specimen

Context Map And High-Magnification Images Stored in a Single File

The metadata images used in the camera’s mapping feature
can also be used as stand-alone images. In addition, the
entire overview map and high-magnification image can be
saved and exported in a common file format for enhanced
flexibility. Users are able to place markers in real-time on the
overview map to highlight potential areas of interest, making
it easy to go back and re-image previously viewed areas.

5
Focus on Dim Fluorescent Signals with Intelligently Designed Noise Reduction
The multi-frame live noise reduction (FNR) feature makes it possible to capture low-noise, high-speed images of live
specimens in real time. Olympus’ noise reduction technology enables users to obtain sharp fluorescence images, even in
weak fluorescence. The FNR feature takes multiple pictures of an image and then removes the random background noise.
The camera is intelligent enough to be able to stop the FNR only when the stage is moving, making it easier to scan your
specimen and focus during fluorescence imaging.

Maintain 15 fps using high gain The FNR automatically starts after Customers can focus on live images
the stage stops with suppressed background noise

Super Fluorescence (SFL) Mode Optimizes for Correct Exposure

The DP74 camera is equipped with SFL mode and is
optimized for fluorescence observation. With SFL, the
camera automatically sets the optimal exposure time to
capture balanced fluorescence images, without losing
details to overexposed areas.

Cultured cell (MDA-MB-231) transduced with multiple fluorescent proteins. Image

data courtesy of [Link] Mishima, The Cancer Chemotherapy Center, Japanese
SFL Without SFL Foundation for Cancer Research

The Auto Expose Feature Helps Prevent Overexposure When Changing Objectives
The DP74 fluorescence color camera automatically detects when a user changes objectives and waits until the image is
uniformly illuminated before performing auto exposure. This helps prevent acquiring images that are overexposed and facilitates
a smooth transition from one objective to the next.

6
DP74 Specifications DP74 System Requirements
Item Specifications Item PC/AT compatible
Camera type Single chip color CMOS (pixel shifting) Intel Core i5, Intel Core i7, Intel Xeon, (or
CPU
Cooling system: Peltier device equivalent)
Imaging Sensor Size 1/1.2 inch 2.35-megapixel color CMOS Global Shutter RAM*5 4 GB or more (dual-channel)
Camera mount C-mount PCI Express X4 Rev.2.0 or later
Extension
5760 × 3600 (pixel shifting, 3-CMOS mode) Half-size, low-profile PCIe board
slot*6
2880 × 1800 (pixel shifting, 3-CMOS mode) compatible (106.7 mm × 174.6 mm)

1
1920 × 1200 (1 × 1, 3-CMOS mode) Microsoft Windows 10 Pro (64 bit)
Effective image resolution*
1920 × 1080 (1 × 1), 1600 × 1200 (1 × 1) Microsoft Windows 8.1 Pro (64 bit)
960 × 600 (1 × 1, 2 × 2) OS Microsoft Windows 7 Ultimate /
ROI Professional SP1 (32 bit / 64 bit)
Sensitivity 0.5X/1X/2X/4X/8X/16X (ISO 200 / 400 / 800 / 1600 / 3200 / 6400 equivalent) Language: English / Japanese
A/D 12 bit *1 Pixel shift and 3-CMOS mode images cannot be acquired in a
32-bit OS environment.
Metering Mode Auto, SFL-Auto, Manual *2 Frame rate may decrease depending on the condition of your
modes Adjustment ±2.0 EV step: 1/3 EV PC and/or software.
*3 Monitor designed to meet Adobe RGB is required.
Time 39 μs to 60 s
*4 Not available in the combination of cellSens Entry and DP2-
Metering modes Full image, 30%, 1%, 0.1% TWAIN.
*5 In order for the maximum full-size live frame rate to be
Binning 2×2 achieved, both dual-channel and PC10600 or greater RAM is
Live frame rate 1920 × 1200 (1 × 1): 60 fps*2, 1920 × 1080 (1 × 1): 60 fps*2 required.
*6 Do not install large boards, like graphic boards, in the adjacent
Still image transfer time 5760 x 3600 (3 × 3): approx. 4 s slot on the fan side of the DP74 camera's PCIe board. Please
contact your nearest Olympus dealer for more information.
1920 x 1200 (1 × 1): approx. 0.3 s
Color space sRGB, AdobeRGB*3
Image file format File formats supported by cellSens software
Position navigator*4 Available
Dimensions Camera interface
cable Approx. 2.5 m

DP74 System Diagram DP74 Camera Head Dimensions

DP74

է116
Monitor
DP74 Camera
Camera Head Interface Cable PCIe I/F Board Desktop
PC
87.7

cellSens
Low-Profile Software
Bracket
DP2-TWAIN*

C-mount
Camera Adaptor
*Download the software from our website (unit : mm) Weight : approx. 1.1 kg

Immunofluorescence image of cynomolgus postimplantation embryo (16 days old), Red:OCT4,

Green:SOX11
Image data courtesy of : Prof. Mitinori Saitou and Dr. Tomonori Nakamura, Anatomy and Cell
Biology, Medicine and Medical Science , Graduate School of Medicine Kyoto university
(Cover page , left)

• is ISO14001 certified.
• is ISO9001 certified.
• All company and product names are registered trademarks and/or trademarks of their respective owners.
• Images on the PC monitors are simulated.
[Link] • Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer.

Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

N8600591-102016
 Imaging Software

cellSens

Intuitive Operation.
Seamless Workflow.

For research and

clinical research
applications

Not for clinical diagnostic use.

More Time for Research
Whether you work in a lab or are conducting complex research experiments, cellSens software
gives you the control to create a seamless workflow tailored to your needs. Find all the tools you
need in one place to focus on research and get results quickly.

Image Personalize Process

All camera controls are Intuitive at all skill levels, the cellSens Prepare your images for analysis
conveniently grouped in one user interface can be fully customized with powerful tools such as
toolbar for efficient imaging. to your current experiment and easily deconvolution, background
Whether you’re capturing a adapts as your application needs subtraction, flatfield correction,
single image or imaging in six evolve. Choose from premade layouts image stitching, spectral unmixing,
dimensions, you can easily or build your own. and various Z-stack displays
accomplish your work using (including maximum intensity
a single software package. projections).

Analyze
Powerful analysis tools enable you to extract data from
your images for quantification. Generate simple or complex
statistics, make confluency measurements, or export to Excel®
for further analysis. In addition, the software’s deep-learning
technology offers improved segmentation analysis. The original
images remain unaltered after extraction, so you can feel
confident in your research integrity.

Choose the Version That Suits Your Application

Entry Standard Dimension
Suitable for lab workers or If your experiments involve Our most advanced microscope
researchers who primarily fluorescence imaging, cellSens imaging solution, cellSens
undertake single-shot acquisition, Standard is a cost-effective Dimension includes the basic
cellSens Entry offers simple solution. With all the features of features of Entry and Standard
layouts that make it easy to Entry, Standard adds powerful and adds functionality for
find all the tools you need. For tools like the acquisition of 3D researchers engaged in complex
collaboration, Conference Mode images that combine XY, Ch, T, imaging experiments. It flexibly
maximizes images that appear and multiple points (stitching), as supports various applications,
on the screen during wireless well as the ability to overlay multi- such as 6D image acquisition that
streaming, while annotation tools color images and perform simple freely combines XY, Z, Ch, T, and
make it easy to highlight areas object count with just one click. multiple points (stitching), as well
of interest and collaborate with as image processing, luminance
colleagues around the world. analysis, and colocalization
analysis. You can also execute
image processing and analysis
with one click.
Specifications
cellSens Solutions Included Optional Entry Standard Dimension
Easily create a high-resolution composite image (Instant MIA) by simply moving
Manual
the manual stage. You can also acquire a focused image (EFI) over the entire
Process surface by manually shifting the Z direction.
Encoded
Encoded devices (objectives, light intensity, etc.) make it easy to recall settings.
Device
Interactive Draw a polyline, rectangle, or circle on top of your image to obtain exportable
Measurement measurement data. Measurement results can be exported to Excel.
Database
Access to the database created with the Database Core option.
Client
Confluency Determine the confluency of unstained live cells in culture dishes through
-
Checker quantitative measurements for reliable data.
Multipoint and stitched images can be acquired using the motorized stage.
When combined with the motorized Z, a focus map can be created from multiple
Multiposition points of focus, and you can obtain stitched images with little focus deviation by
-
removing sample tilt and distortion.
Count & Define the morphology of an object, and the software will identify all similar
-
Measure objects and present segmentation analysis results in a chart.
Make data management and browsing more efficient by creating a database
Database Core that can easily search and sort acquired images based on data, such as imaging -
conditions and acquisition date.
Facilitates the transfer of live and stored images through a network for teaching,
NetCam mentoring, or supervision.
-

Efficient segmentation analysis powered by deep learning enables challenging

Deep Learning target detection, such as label-free nucleus detection.
-

Easily set the capture settings for each well. The well position and name can
Well Plate
be tagged to images, making data management easier and well plate screening - -
Navigator*1 more efficient.
Access to GPU based deconvolution as well as popular and custom TruSight
CI
deconvolution algorithms to improve the sharpness, contrast, and dynamic - -
Deconvolution range of reconstructed images.
Ratio/FRET Obtain ratio measurements from your images as they’re being acquired. - -
Measure and analyze the luminance and speed of individual cells that move and
Tracking*2 divide over time.
- -

Life Science
FRAP / FRET analysis can be performed on the acquired image. - -
Analysis
Photo
Enables cell frap module control and FRAP analysis. - -
Manipulation
*1 Requires Multiposition option *2 Requires Count & Measure option

cellSens Functions
Dimension Standard Entry
Layout User experience customization • • •
Overlay multiple images • • -
Document groups for side-by-side image comparison • • •
Movie playback • • •
View
Tile view (multiple images in a single data set shown side-by-side) • • •
Slice view for orthogonal plane viewing of 3D or time-lapse data sets • - -
Voxel viewer for isosurface and volumetric rendering of 3D and 4D data sets • - -
Snap/movie acquisition • • •
Time-lapse at specified interval • • -
Automated multiwavelength • • -
Z-stack • - -
Multidimensional (XYZT and wavelength) • - -
Graphical Experiment Manager • - -
Manual panoramic imaging (Instant MIA and Manual MIA) • Manual Process Manual Process
Image Multiposition visitation and stage navigator Multiposition Multiposition -
Acquisition Automated panoramic imaging (Auto MIA, requires motorized stage) Multiposition Multiposition -
Instantly create EFI image (manual or motorized Z) • Manual Process Manual Process
Simultaneous multicolor Imaging
• - -
(requires two identical cameras** or image splitter)
Live deblurring • - -
High dynamic range imaging (HDRI) • - -
Well Plate Navigator
Multiwell plate acquisition - -
and Multiposition
Geometry/combine/filter processing • • -
Fluorescence unmixing • - -
Brightfield unmixing Count & Measure - -
Image
Deblurring (No/Nearest Neighbor, Wiener Filter) • - -
Processing
Kymograph • - -
2D deconvolution • - -
3D deconvolution (constrained iterative deconvolution with GPU processing) CI Deconvolution - -
Training of Neural Networks Deep Learning Deep Learning -
Deep Learning Deep Learning or Deep Learning or
Inference using trained Neural Networks (offline/online) -
Count & Measure Count & Measure
cellSens Functions
Dimension Standard Entry
Region and line measurements • • -
Phase analysis • - -
Object analysis and classification Count & Measure Count & Measure -
Interactive measurement • • •*
Intensity plot over time/z • - -
Colocalization • - -
Object counting (manual) • • -
Tracking and Count &
Image Analysis Object tracking - -
Measure
Online ratio and kinetics Ratio/FRET - -
Ratio analysis (offline) • - -
Ratio/FRET or Life
FRET analysis - -
Science Analysis
Photo Manipulation or
FRAP analysis - -
Life Science Analysis
Cell count and confluency measurements • Confluency Checker -
Automatically compose MS Word reports • - -
Documentation
Database image and data management solution for microscopy Database Core Database Core -
and Collaboration
Open database and load records/documents from database Database Client Database Client Database Client
Remoting Remote live image viewing NetCam NetCam -
*Three points angle, four points angle, arbitrary line, closed polygon, polyline and perpendicular line only. Interactive measurement option is needed to add other measurement tools and make exporting Excel spreadsheets possible.
**Supported cameras: iXon Ultra 897, Zyla 5.5 (USB 3.0), Zyla 4.2 (USB 3.0/CamLink), Neo, iXon Ultra 888, ImagEM X2, ORCA-Flash 4.0 (V2/V3), Prime 95B, Prime BSI, Sona4.2B-11, ORCA-Fusion, ORCA-Fusion BT

Products with Confirmed Functionality

Dimension Standard Entry
DP22, DP23, DP27, DP28, DP73, DP74, DP80, XM10, XC10, XC30, XC50,
Camera • • •
UC30, UC50, UC90, LC20, LC30, SC30, SC50, SC100, SC180
BX43, BX53, BX63, BX61, BX61WI, IX83, IX73, IX81, SZX16A • • -
Micoscope
IX81-ZDC, IX81-ZDC2 • - -
Olympus BX-DSU, IX3-DSU, IX3-ZDC, IX3-ZDC2, IX2-DSU, IX2-ZDC, IX2-ZDC2,
Peripherals U-CBF, cellTIRF (multiline, single line), MT20, USB-ODB converter, Real Time • - -
Controller (U-RTC and U-RTCE), U-FCB, U-STC
Motorized XY Stage BX3-SSU, IX3-SSU Multiposition Multiposition -
Light Source U-LGPS • • -
ORCA R2, ORCA-Flash 2.8, ImagEMX2, ORCA-Flash 4.0 V2, ORCA-Flash
4.0 V3, ORCA-Flash 4.0 LT,ORCA-Flash 4.0 LT PLUS, ORCA-Fuision, ORCA- • - -
Camera
Hamamatsu Fusion BT
ORCA-Spark • • -
Image Splitter W-View Gemini • - -
Q-Imaging Camera Retiga 6000 • - -
Camera CoolSNAP HQ2, Prime (PCI-Express), Prime 95B, Prime BSI, Moment • - -
Photometrics
Image Splitter Dual View DV2 / QuadView QV2 • - -
iXon X3 897, iXon Ultra 897, iXon Ultra 888, iXon Life 888, iXon Life
Andor Camera 897,Sona4.2B-11 Zyla4.2/Zyla4.2 PLUS (Camera-link,USB3.0), Zyla5.5 • - -
(Camera-link 10tap,USB3.0), Neo 5.5
Vincent Associates Shutter Uniblitz shutter (VCM-D1, VMM-D1, VMM-D3) • • -

pE-1, pE-2, pE-4000,pE-340fura • - -

CoolLED Light Source
pE-300white, pE-300ultra, • • -
X-Cite 120 PC, X-Cite exacte, X-Cite110LED, X-Cite120LED, X-Cite XYLIS,
Excelitas Light Source • - -
X-Cite TURBO
Lumencor Light Source SOLA SEII, SEII 365, Spectra X • - -
Light Source Lambda DG4 • - -
Sutter
Shutter, FW Lambda 10-3/10-B • - -
Motorized XY Stage ProScan III , Optiscan III Multiposition - -
Shutter, FW, Z-drive ProScan (I, II, III) , Optiscan III • - -
Prior
Piezo Z (Control via
NanoScanZ NZ100 • - -
Real Time Controller)
Motorized XY Stage Mac 6000 Multiposition - -
Ludl
Shutter, FW, Z-drive Mac 6000 • - -
Motorized XY Stage
Oasis 4i Multiposition - -
Objective Imaging Controller
Z-drive Controller Oasis 4i • - -
Motorized XY Stage Tango, Pilot Stage Multiposition - -
Märzhäuser
Z-drive Controller Tango • - -
Piezo Z (Control via
Physik Instrumente PIFOC P-721 • - -
Real Time Controller)
Applied Scientific Motorized XY Stage MS-2000 Multiposition - -
Instrumetation Z-drive Controller MS-2000 • - -
National Instruments Digital TTL Device NI USB-6501 • - -
Yokogawa CSU CSU-X1, CSU-W1 • - -

Compatible image formats System requirements

JPEG, JPEG2000, TIFF, OS* Microsoft Windows 10 Pro (64-bit) Microsoft Windows 8.1 Pro (64-bit)
Read
BMP, AVI, PNG, VSI, PSD OS Language English, Simplified Chinese, Japanese, German, Russian (Entry and Standard) and Italian (Entry and Standard)
and
(Adobe Photoshop), Big TIFF, CPU Intel Core i5, Intel Core i7, Intel Xeon Recommended for high-speed image acquisition: QuadCore
write
OIR (FLUOVIEW format) RAM 4GB for general applications, 8GB or more is recommended for high-speed image acquisition
GIF, OIF/OIB (FLUOVIEW 1 GB for installation
HDD
Read format), Cell, STK Recommended for high speed image acquisition: Solid State Drive (SSD)
only (MetaMorph), MRC (Medical Web Browser Recommended: Microsoft Internet Explorer 11
Research Council)

• is ISO14001 certified.
• is ISO9001 certified.

[Link]
• All company and product names are registered trademarks and/or trademarks of their respective owners.
• Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer.

Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

Printed in Japan N8601749-042021