SAMPLE MICROBIOLOGY QUESTIONS
PART 1.
1. When the number of the microbes that die in a culture is equal to the number that is produced, the
cells are in the ..
a. Exponential phase c. Lag phase
b. Death phase d. Stationary phase
2. When the number of microbial cells in a population neither increases nor decreases, the microbes are
in the ...
a. Exponential phase c. Lag phase
b. Death phase d. Stationary phase
3. One method to measure number of microbes indirectly is by...
a. Direct techniques. c. Indirect counting
b. Filtration techniques. d. Turbidity techniques
4. Mesophiles are a major cause of human disease because ...
a. They are able to survive at human body temperature
b. They cannot be seen in contaminated food.
c. They are abke to grow at dangerous body temperature
d. They are able to grow at 0°C - 15°C
5. Obligate anaerobes ;
a. Require only Carbon dioxide for growth
b. Require only Oxygen and high amount of Carbon dioxide
c. Require less Oxygen and high amount of Carbon dioxide
d. Require more Oxygen and small amount of Carbon dioxide
6. Which of the following is not one method by which microbes multiply ?
a. Binary fission. c. Budding
b. Asexual reproduction d. Sexual reproduction
7. All the folkowing microscopy produce 2D images except ...
a. Bright-field microscopy c. Phase-contrast microscopy
b. Differential microscopy. d. Transmission electron
8. High quality resolution is obtained using light of a ...
a. Faster wavelength. c. Shorter wavelength
b. Longer wavelength. d. Slower wavelength
9. The image seen by the eye in a microscope is always a/an...
a. Real image c. Circulare image
b. Inverted image. d. Colored image
10. The first part of the microscope that magnifies the object is the ...
a. Condenser c. Oculare lens
b. Diaphragm. d. Objective lens
11. In order for microbes to be seen in the laboratory by the aide of a microscope, the microbe has to be
;
a. Magnified. c. Increased
b. Expanded. d. Resolved
12. Microbes that are able to grow only at extreme temperature are known as ....
a. Mesophiles c. Neutrophiles
b. Thermophiles d. Psycrophiles
13. Microbes that are not affected by the amount of Oxygen present are called ..
a. Facultative anaerobes c. Obligate anaerobes
b. Microaerophiles d. Obligate intracellular
14. Which of the folowing is not a physical growtg factor?
a. Moisture. c. Temperature
b. pH. d. Vitamin
15. A microscope that has a total magnification of 1, 500X when using the oil immersion objective has
ocular of what power ?
a. 15X. b. 1. 5X. c. 30X. d. 150X
16. Edward Jenner is best known for what contribution to the developing field of microbiology?
a. Developing some of the earliest vaccines
b. Disproving spontaneous generation
c. Controlling infections in patients
d. Developing methods of obtaining pure culture of microorganisms
17. In which of the following grouos would you find mushrooms?
a. Bacteria. b. Protozoa. c. Fungi. d. virus
18. Microbiology studys all the following organisms except...
a. Bacteria. b. viruses. c. Plants. d. Protozoa
19. A substance derived from one microorganism that kills/restricts the growth of other microorganisms
is known as ....
a. Poison. b. Antibody. c. Vaccines. d. Antibiotics
20. The concept of putting microbes to work to clean up the environment is called ....
a. Bioremediation. b. Pasteurization. c. Immunization. d. Fermentation
PART 2
1. Explain the following ...
a. Microbial growth
b. Generation time
2. List 2 types of light microscope.
3. List 4 physical growth factors that are required by microbes for ootimum growth.
4. Why is it that microbes in the lag phase do not multiply ?
5. Differentiate between lag phase and stationary phase.
6. What is the total magnification of the image formed by a microorganism?
7. State 2 conditions that could cause stationary phase of microorganism.
8. Preparation that establishes immunity to disease is called ____________.
9. A total of (10) E coli cells have contaminated your water today. Ifvthe organism has a generation time
of 20 minutes
a. How many generations woukd be present after 1 hour ?
b. How many cells would be present after 1 hour ?
show working...
PART 3
1. In order to view a causative agent , a microbiologist will use a/an _________.
2. If the study of algae is phycology , then _____ is the study of fungi.
3. Living specimen can only be examined by a /an _________ microscope.
4. For a doubling time of 15 minutes, 25 bacteria cells will increase to ______ after 30 minutes.
5. The function of the mechanical stage of the microscope is _______.
6. _______ mounts are made by drying and heating specimen.
7. The process by which parent cell divide into ( 2 ) daughter cells is known as _____.
8. Organisms that can also grow in refrigerator are also called_____.
9. Anaerobes that have mechanisms to detoxify Oxygen are called _________.
10. Gas production by microbes is one indirect way of measuring number of microbes by _____ activity.