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Chapter Three Project

The document presents results from experiments on various magnetic nanoparticles, detailing their antioxidant activities and IC50 values across different concentrations. It includes data on ABTS, DPPH, TBARS, hydrogen peroxide, heat-induced hemolysis, albumin denaturation, total antioxidant capacity, reducing power, and nitric oxide scavenging activity. Each section provides specific concentration values and corresponding inhibition percentages or antioxidant capacities, along with regression equations and R² values for analysis.

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David Chieme
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6 views6 pages

Chapter Three Project

The document presents results from experiments on various magnetic nanoparticles, detailing their antioxidant activities and IC50 values across different concentrations. It includes data on ABTS, DPPH, TBARS, hydrogen peroxide, heat-induced hemolysis, albumin denaturation, total antioxidant capacity, reducing power, and nitric oxide scavenging activity. Each section provides specific concentration values and corresponding inhibition percentages or antioxidant capacities, along with regression equations and R² values for analysis.

Uploaded by

David Chieme
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

CHAPTER THREE

RESULTS.

80
70 f(x) = 0.52375 x + 21.531
R² = 0.885583454972408
60
50
ABTS (%)

40
30
20
10
0
10 20 30 40 50 60 70 80 90 100 110
Concentrations (mg/ml)

ABTS Magnetic Nano-particle

IC50 value= 0.283

Concentration
20 mg/ml 87.80 ± 10
40 mg/ml 83.07 ± 14.48
60 mg/ml 83.56 ± 14.19
80 mg/ml 81.93 ± 15.60
100 mg/ml 73.96 ± 22.46
DPPH magnetic Nano-particle
60
Lipid peroxidation inhibition (%)
50

40 f(x) = 0.44532 x + 0.0253999999999976


R² = 0.757505468412169
30

20

10

0
10 20 30 40 50 60 70 80 90 100 110
Concentrations (mg/ml)

TBARS Magnetic Nano-particle

IC50 value= 0.006

Concentration
20 mg/ml 45.79 ± 0.09
40 mg/ml 27.74 ± 0.09
60 mg/ml 31.03 ± 0.09
80 mg/ml 13.95 ± 0.09
100 mg/ml 6.10 ± 0.24
Hydrogen peroxide Magnetic particles

IC50 value= 6.85

Concentratio
n
20mg/ml 99.25 ± 0
40mg/ml 98.83 ± 0
60mg/ml 99.00 ± 0
80mg/ml 98.50 ± 0
100mg/ml 98.72 ±0.05
Hypotonic Magnetic Nano-particle
97.6
Heat haemolysis inhibition(%) 97.4
97.2
f(x) = 0.00984999999999999 x + 96.241
97 R² = 0.398990418225929
96.8
96.6
96.4
96.2
96
95.8
10 20 30 40 50 60 70 80 90 100 110
Concentraions (mg/ml)

Heat –induced magnetic Nano-particles

80
Albumin Denaturing activity (%)

70
f(x) = 0.6165 x + 6.31800000000001
60 R² = 0.933402550803854
50
40
30
20
10
0
10 20 30 40 50 60 70 80 90 100 110
Concentrations(mg/ml)

Albumin magnetic Nano particle


12
Total Antioxidants Capacity (AVR)
10

0
40 mg/ml 60 mg/ml 80 mg/ml 100 mg/ml

Concentrations (mg/ml)

TAC Magnetic Nano particle

80
70
Reducing Power (AVR)

60
50
40
30
20
10
0
20 mg/ml 40 mg/ml 60 mg/ml 80 mg/ml 100 mg/ml
Concentrations (mg/ml)

FRAP Magnetic Nano-particles


Nitric Oxide scavenging activity 88

86

84

82
(%)

80

78

76

74
20 mg/ml 40 mg/ml 60 mg/ml 80 mg/ml 100 mg/ml
Concentrations (mg/ml)

Nitric Oxide Magnetic Nano-particles


REFERENCE
1. Okafor, C. E., Ijoma, I. K., Igboamalu, C. A., Ezebalu, C. E., Eze, C. F., Osita-Chikeze, J.
C., Uzor, C. E., & Ekwuekwe, A. L. (2024). Secondary metabolites, spectra
characterization, and antioxidant correlation analysis of the polar and nonpolar extracts
of Bryophyllum pinnatum (Lam) Oken. Biotechnologia, 105(2), 121–136.
[Link]

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