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Bio Chemical Engineering Exam: Yeast Growth Analysis

The document outlines a take-home exam for a Bio Chemical Engineering course, with problems focused on yeast growth in chemostat and batch fermenters using glucose as a substrate. It includes calculations for cell growth rates, biomass production, and substrate conversion in various fermenter configurations. The exam is due on March 2, 2025, at 2 PM and is assigned to a student named Yonatan Endeshaw Ejigu.

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Elias Biramo
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5 views2 pages

Bio Chemical Engineering Exam: Yeast Growth Analysis

The document outlines a take-home exam for a Bio Chemical Engineering course, with problems focused on yeast growth in chemostat and batch fermenters using glucose as a substrate. It includes calculations for cell growth rates, biomass production, and substrate conversion in various fermenter configurations. The exam is due on March 2, 2025, at 2 PM and is assigned to a student named Yonatan Endeshaw Ejigu.

Uploaded by

Elias Biramo
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

Bio Chemical Engineering Home Take Exam

Due Date: 3/2/25 BF 2PM

UGR/8327/13 YONATAN ENDESHAW EJIGU yonathanendeshaw@[Link]

Problem 1: For laboratory experiment results of a chemostat study on the growth of a specific strain of
baker’s yeast using glucose as a limiting substrate as shown in the following table. The inlet
concentration of glucose was set at 100 g/L. The volume of the fermenter contents was 500 mL. The
inlet stream was sterile. If the strain growth rate is expressed using Monod kinetics and the yield
constant Yx/s is 0.6, find the rate equations for cell growth.

Feed Flow Rate(F), Steady State


ml hr-1 Substrate Conc.(S)
g/L
123 25
122 20
119 15
114 10

Problem 2: A yeast strain biomass in a problem 1 is produced in a batch fermenter using glucose as a
limiting substrate with yield of biomass (Yx/s) is 0.6 g of dry cells per gram of glucose. If 5 g dry cells are
inoculated into 50 litres of medium containing 12 g per liter glucose. Determine batch culture times
required to:

(a) Produce 10 g biomass;

(b) Achieve 90% substrate conversion.

Problem 3: If you cultivate the yeast strain in problem 1 in a steady-state CSTF, where F = 100 L/hr, Si =
50 g/L, and Yx/s = 0.6.

a. What size vessel will give the maximum total rate of cell production?

b. What are the substrate and cell concentrations of the optimum fermenter in part (a)?

c. If the exiting flow from the fermenter in part (a) is fed to a second fermenter (CSTF), what should be
the size of the second fermenter to reduce the substrate concentration to 1 g/L?

d. If the exiting flow from the first fermenter in part (a) is fed to a second fermenter whose size is the
same as the first, what will be the cell and substrate concentrations leaving the second fermenter?

Problem 4: A strain of yeast in problem 1 is being cultivated in a 30-L CSTF with a cell recycling system
(cell settler) as shown in the following figure. The cell settler was designed so that the cell concentration
of its outlet stream is 30 percent of that of its inlet stream, whereas the substrate concentrations of the
two streams are the same. Calculate the steady-state substrate and cell concentrations in the fermenter.
The inlet substrate concentration is 100 g/L and the flow rate is 20 L/hr. The feed stream is sterile

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