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Instrumental Analysis Methods Overview

The document outlines a series of questions related to Instrument Method of Analysis for a 7th semester course, covering various analytical techniques such as Beer-Lambert's Law, HPLC, chromatography, and spectroscopy. It includes both 5-mark and 10-mark questions that require explanations of principles, instrumentation, applications, and various factors affecting the methods discussed. Topics range from flame emission spectrometry to electrophoresis and the principles of different types of chromatography.

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Subhrajit Nayak
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12 views1 page

Instrumental Analysis Methods Overview

The document outlines a series of questions related to Instrument Method of Analysis for a 7th semester course, covering various analytical techniques such as Beer-Lambert's Law, HPLC, chromatography, and spectroscopy. It includes both 5-mark and 10-mark questions that require explanations of principles, instrumentation, applications, and various factors affecting the methods discussed. Topics range from flame emission spectrometry to electrophoresis and the principles of different types of chromatography.

Uploaded by

Subhrajit Nayak
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

7TH SEMESTER

INSTRUMENT METHOD OF ANALYSIS


5 MARKS
1. Write statement & derive equa on for beer’s lawberf’s law
2. Derive Beer and Lambert’s Law. What are the devia ons observed from Beer Lambart’s law
3. Explain instrumenta on and working of flame emission spectrometry
4. What are nephelometry & turbidometry? write the principle involved for the same
5. Instrumenta on and applica on of HPLC
6. Discuss the different types of detectors used in HPLC.
7. Rate and plate theory
8. Explain the concept of plate theory and rate theory for increasing the efficiency of column in chromatography.
9. Principle, theory and applica on of gel chromatography
10. Enlist and explain the various development techniques in paper chromatography
11. Discuss the detectors used in Gas Chromatography.
12. What is Affinity chromatography? Describe the theory and applica ons of Affinity chromatography.
13. Explain the principle of Atomic Absorp on spectroscopy.
14. Describe the procedure for the prepara on of column for column chromatography.
15. What is quenching? various factors influencing quenching effect
16. What is electrophoresis? Various factors affec ng electrophoresis
17. Explain the monochromators and detectors of UV-Visible spectrophotometer.
18. Write the different methods for prepara ons of TLC plates.
19. What are ion exchange resins? Classify and explain the ideal proper es of ion exchange resins.
20. Define and dis nguish between fluorescence and phosphorescence. Write the various factors affec ng the
phenomenon of fluorescence.
21. Discuss the principle & applica ons of Flame photometry
22. Discuss the principle of Flourimetry and factors influencing Fluorescence intensity in Flourimetry.
23. Describe briefly Van Deemter equa on and plots.

10 MARKS

1. Describe instrumenta on, principle and applica on of UV-visible spectrophotometer


2. Note on theory and applica on of IR spectrophotometry, explain different sampling techniques employed
3. Discuss the different sources of radia ons & detectors used in IR spectroscopy
4. Describe instrumenta on, principle and applica on of GAS chromatography. requirements of deriva za on of
samples in gas chromatography.
5. Describe principle and different techniques of PAPER electrophoresis. Add a not on applica on
6. Describe and derive the equa on for Beer’s - Lambert’s law. note on devia ons and limita ons of beers law.
7. Describe the principle, instrumenta on and applica ons of HPLC
8. Describes the principle, working and instrumenta on of AAS. Add a note on its applica ons.
9. Describe the principle and different types of paper chromatography. Discus the common developing reagents
used for detec on of components in paper chromatography.

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