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Enzyme Inhibition Mechanisms Explained

The document outlines the mechanisms of enzyme inhibition, detailing competitive, non-competitive, and uncompetitive inhibition. It includes descriptions of how inhibitors interact with enzymes and the effects on reaction kinetics, illustrated through equations and plots. Additionally, it provides insights into how varying substrate concentrations influence inhibition outcomes.

Uploaded by

Razan Fakih
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14 views1 page

Enzyme Inhibition Mechanisms Explained

The document outlines the mechanisms of enzyme inhibition, detailing competitive, non-competitive, and uncompetitive inhibition. It includes descriptions of how inhibitors interact with enzymes and the effects on reaction kinetics, illustrated through equations and plots. Additionally, it provides insights into how varying substrate concentrations influence inhibition outcomes.

Uploaded by

Razan Fakih
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Enzyme Inhibition (Mechanism)

Competitive Non-competitive Uncompetitive


Substrate E
Cartoon Guide

Compete for
Inhibitor active site Different site

E + S←
→ ES → E + P E + S←→ ES → E + P E + S←
→ ES → E + P
Equation and Description

+ + + +
I I I I
↓↑ ↓↑ ↓↑ ↓↑
EI EI + S →EIS EIS
[I] binds to free [E] only, [I] binds to free [E] or [ES] [I] binds to [ES] complex
and competes with [S]; complex; Increasing [S] can only, increasing [S] favors
increasing [S] overcomes
not overcome [I] inhibition. the inhibition by [I].
Inhibition by [I].

Juang RH (2004) BCbasics

Enzyme Inhibition (Plots)

Competitive Non-competitive Uncompetitive


Vmax Vmax Vmax
vo vo
Vmax’
Direct Plots

Vmax’
I I I

Km Km’ [S], mM Km = Km’ [S], mM Km’ Km [S], mM


Vmax unchanged Vmax decreased
Km increased Km unchanged Both Vmax & Km decreased
Double Reciprocal

1/vo I 1/vo I 1/vo


I
Two parallel
Intersect lines
at Y axis 1/ Vmax Intersect 1/ Vmax 1/ Vmax
at X axis

1/Km 1/[S] 1/Km 1/[S] 1/Km 1/[S]

Juang RH (2004) BCbasics

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