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Anti-Diabetic and Antimicrobial Properties of Pithecellobium dulce

The document discusses the emergence of carbapenem-resistant Acinetobacter baumannii and the characterization of the OXA-51 β-lactamase enzyme. It highlights the potential of herbal and nano-based alternatives for treating infections caused by this resistant strain, specifically noting the antimicrobial activity of PVP-capped silver nanoparticles and extracts from Aegle marmelos and Calotropis procera. The research suggests that older antibiotics like penicillin may be effective against NDM-harboring A. baumannii.

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4 views1 page

Anti-Diabetic and Antimicrobial Properties of Pithecellobium dulce

The document discusses the emergence of carbapenem-resistant Acinetobacter baumannii and the characterization of the OXA-51 β-lactamase enzyme. It highlights the potential of herbal and nano-based alternatives for treating infections caused by this resistant strain, specifically noting the antimicrobial activity of PVP-capped silver nanoparticles and extracts from Aegle marmelos and Calotropis procera. The research suggests that older antibiotics like penicillin may be effective against NDM-harboring A. baumannii.

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itsdoremi088
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© All Rights Reserved
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Oral/ Poster

Lupane, coumarins and prenylated flavonoids from bark of Pithecellobium dulce


and in vitro screening for anti-diabetic potential

Hassan AN1, Mir SR2


1
School of Biological Sciences and Biotechnology, Institute of Advanced Research, Koba Institutional Area, Gandhinagar-
382007, India.
2
MNPRL, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai-
400019, India
E-mail ID: abcd@[Link]

Acinetobacter baumannii causes nosocomial infections and its prevalence in the clinical setup
has increased gradually. Commonly prescribed drug against A. baumannii is carbapenem that
belongs to the β-lactam group of antibiotics. A. baumannii has emerged resistance against
carbapenem, which is a significant health problem and responsible for high morbidity and
mortality. Phenotyping (Iso-electric focusing), genotyping (PCR and sequencing) and
quantitative proteomics (DIGE and Mass Spectrometry) studies concluded the overproduction
of different carbapenem hydrolyzing β-lactamase (OXA-51, AmpC and NDM) and up-regulation
of metabolism in carbapenem resistant strain. Selected protein i.e OXA-51 was cloned, purified
and characterized using different biophysical methods such as CD, fluorescence and ITC. The
result shows that recombinant OXA-51 has and temperature change. We have also signified the
use of excitation at 305nm for monitoring the surface tryptophan of protein. Bioinformatics
studies on the modeled OXA-51 showed that carbapenem is effectively hydrolyzed by OXA-51.
Using in-silico we have also pointed out that old β-lactam such penicillin might be better
antibiotic for NDM-harboring A. baumannii. We have separately tested two alternatives (i.e
herbal-based, and nano-based) for carbapenem resistant strain of Acinetoabcter baumannii. We
have tested polyvinylpyrrolidone (PVP), sodium citrate, chitosan, sodium dodecyl sulfate
capped silver nanoparticles and found that PVP capped AgNPs shown antimicrobial activity.
Similarly, polar extracts from Aegle marmelos and Calotropis procera have independently shown
antimicrobial activity against resistant strain (RS307) of A. baumannii. Therefore, they can be
used to develop an alternative to carbapenem, the most prescribed antibiotics for the
Acinetobacter baumannii infections.
Acknowledgement(s): DST and University of Delhi and UGC for fellowship.
References: 1. Corsi MM, Bertelli AA, Gaja G. Int J Tissue React 1998;20:115–8; 2. Pecere T, Gazzola MV, Mucignat C,
Parolin C, Vecchia FD, Cavaggioni A, et al. Cancer Res 2000;60:2800–4; 3. Yi Mei Zhenga, James Zheng Shenb, Yan
Wangb, Amy Xiaoxu Lub, Wing Shing Ho. Phytomedicine 000 (2016) 1-8; 4. Márcio Carocho and Isabel C.F.R. Ferreira.
Anti-Cancer Agents in Medicinal Chemistry, 2013, 13, 1236-1258. © 2013 Bentham Science Publishers.

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