Undergraduates Biology (UG-BIO)- Laboratory Class

BIO103X

Department of Biological Sciences,

Indian Institute of Science Education and Research Bhopal (IISERB)
Indore By-pass Road Bhauri Bhopal, 462066
Madhya Pradesh, India
 Faculties and staffs associated with UG-BIO Lab

1) Dr. Sourav Datta, Associate Professor, Head of the Department (HoD)

2) Dr. Varun Chaudhary/ Dr. Nagarjun Vijay UG BIO Lab In-charge

3) Dr. Pankaj Pandey, Scientific Officer/Instructor

4) Mr. Anbumani D., Scientific Superintendent

5) Ms. Anshulata Bhoite, Scientific Assistant

6) Mr. Yashwant Bhargava, Lab Assistant

Link: [Link]
 BIO-UG LAB TEAM

1) Dr. Pankaj Pandey, Scientific Officer

2) Mr. Anbumani D., Sci. Sup.
3) Ms. Anshulata Bhoite, Sci. Assist.
4) Mr. Yashwant Bhargava, Lab Assist.
5) Mr. Pritam, Lab Attn.

5 2 1 3 4
 Do’s and Don’ts while working in UG BIO Lab Class
Do’s Don’ts
Be on time. Don’t be too shy to ASK QUESTIONS
A lab coat must be worn during laboratory Do not eat, drink, chew gum, in the lab.
experiments. Dress properly during a Just being in lab makes your hands dirtier
laboratory activity. Long hair, dangling than you can imagine and you don't want
jewelry and loose or baggy clothing are a to accidentally eat any reagent
hazard in the laboratory.
Inform the instructor and/or TA if there is a Do not wear open-toed shoes (sandals) in
problem. the lab.
If you do not understand how to use a Don't taste or Sniff Chemicals.
piece of equipment, ASK THE
INSTRUCTOR FOR HELP!
Conduct yourself in a responsible manner Never operate any equipment until you
at all times in the laboratory. have been instructed in its proper use.
Do clean the working place after finishing Never return unused chemicals to the
your experiment. original container.
Wash your hands before you leave the lab. Never IGNORE equipment alarms
 Chemical Safety shower (Emergency Shower)

Eyewash station

Fire extinguisher

If the eyes or body of any person

working inside the laboratory is
exposed to injurious corrosive
materials, suitable facilities for quick
drenching or flushing of the eyes and
body is provided within the lab area
for immediate emergency use.
 LAB NOTE BOOK
➢ Also called as working record and experimental log.

➢ It allows a researcher to summarize his/her scientific thinking .

For purposes of
For scientific process: invention,
it provides the For the purpose of
discovery and
necessary detail so reporting results,
patents, it is the
the lab notebook is
that experiments can primary record used
the repository of all
be repeated and the to establish
primary data and
results can be ownership of an
results
invention and dates
confirmed of discovery
 CONTENT OF THE LABORATORY NOTEBOOK

➢ Table of Content/Index
• Serial number of the experiment
• Name of the experiment
• Page number
• Date of experiment
• Date of submission
• Signature and remarks from Course instructor

➢ Format to be followed while writing any experiment:

AIM—PRINCPLE & THEORY—MATERIAL REQUIRED —PROCEDURE —

OBSERVATION & CALCULATION— RESULTS & DISCUSSION—PRECAUTIONS

AIM/TITLE: Give the experiment a title or what you are going to perform.

PRINCPLE & THEORY: should be a concise statement, in your own words, of what the goal
of the experiment is. This should include the working principle and also have a summary of what
type of data or molecular construct the experiment is expected to produce.

MATERIAL REQUIRED: You should mention the material required before commence the
experiment. You can divide the material into two broad categories, Chemicals &
Plasticwares/glasswares.

PROCEDURE: section should be in outline form. Should describe step by step what you did so
that later you can repeat the experiment exactly the same way. Include key experimental details
as the experiment was performed, not just how you are told to do it in the textbook.

OBSERVATION & CALCULATION: varies from experiment to experiment as some

experiment require microscope and some requires spectrophotometer.

RESULTS & DISCUSSION: this section should include all data gathered in the experiment.
The table or graph generated from the experiment should be clearly pasted. In discussion part the
results should be interpreted or explained. You should present your conclusions as related to the
purpose of the experiment, and the results obtained.

PRECAUTIONS : You should mention the necessary precaution need to be taken while
performing experiment.
 • Lab notebook grades are largely on your results and discussion sections, and
how successfully you demonstrate a clear understanding of the work
1 performed

• Use a bound or spiral notebook and blue or black ink for writing purposes
and pencils for graphs only
• Number ALL pages & DATE all entries
2 • Graphs & images should be glued into the lab notebook and thoroughly
labelled.

• Do not tear out or add pages.

• Do not take lecture notes in your lab notebook. Use a different notebook
3

• Use AIM—PRINCPLE & THEORY — MATERIAL REQUIRED —

PROCEDURE—OBSERVATION & CALCULATION—RESULTS &
4 DISCUSSION—PRECAUTIONS format
 For Online Mode (Tentative)

➢ Lab Introduction (Instrument Details)

➢ Microscopy & Pipette Handling

➢ DNA Isolation at Home

➢ Solution Preparation (Molarity and Percentage solution)
&
Monocot vs Dicot

➢ Qualitative test for Sugars

➢ Quantitative analysis of protein

➢ Introduction to basic bioinformatics

TENTATIVE TEACHING PLAN FOR THIS SEMESTER

Monday: Will be the Lab classes.

Tuesday: Discussion on previous lab class.

• A Google Doc file will be shared at the end of each lab class, where
the students can post their questions and queries related to that
class. Student will have to write his/her name and roll no. before
posting any question and make sure that there is no
repetition/duplication of the question.

• These questions and queries will be discussed in the Tuesday Class

✓ At the end of each lab class, slides will be shared with all students
for better understanding.

✓ After every two lab classes, on-line test will be conducted.

 Semester Grading Scheme
Percentage Activity
70% Quizzes conducted during
semester
30% Biology Project work
Dr. Pankaj Pandey: pankajp@[Link]
Dr. Nagarjun Vijay(In-Charge BIO UG-Lab): nagarjun@[Link]

A Google Class Room Link will be shared shortly, from where you can download
PDF files of delivered lectures and videos links (as the case may be)