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Bioreactor and Cell Culture Experiments

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RAHUL NISHAD
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0% found this document useful (0 votes)
55 views2 pages

Bioreactor and Cell Culture Experiments

Uploaded by

RAHUL NISHAD
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

INDEX

PAGE TEACHER
[Link] EXPERIMENT DATE
NO REMARK
1 Determination of oxygen transfer rate
and volumetric oxygen mass transfer
coefficient (KLa) under variety of 18/09/25
operating conditions in shake flask
and bioreactor.
2 Determination of mixing time and
fluid flow behaviour in bioreactor 23/09/25
under variety of operating condition
3 Rheological study of microbial
cultures and biopolymers- 25/09/25
determination of rheological constant.
4 Production of microbial product using
14/10/25
bioreactor technology.
5 To study the kinetics of an enzymatic
04/11/25
reaction by microorganisms.
6 To produce and purify industrial
importance enzyme (e.g. amylase,
proteinase, ligase or cellulose) from
04/11/25
selected microorganisms using
suitable growth medium and
purification technique.
7 Comparative studies of Ethanol
11/11/25
production using different substrates.
8 Microbial production and downstream 13/11/25
processing of an enzyme- amylase.
9 To immobilize enzymes using 18/11/25
alginate.
10 To study the growth kinetics of 25/11/25
bacteria in a batch culture by plotting
growth curve and determining growth
parameters.
11 Spotting 26/11/25
INDEX
PAGE TEACHER
[Link] EXPERIMENT DATE
NO REMARK
1 To separate and visualize DNA by
07/11/25
agarose gel electrophoresis.
2 Restriction digestion of insert plasmid
07/11/25
or binary vectors.
3 To isolate and culture the cheek embryo
fibroblast under aseptic condition 14/11/25
(Primary cell culture).
4 Preparation of animal cell culture
17/10/25
medium.
5 To determine the viability of cell using
12/09/25
MTT assay.
6 To detection and quantifying apoptosis
in given cell samples using an ELISA 21/11/25
based assay.
7 Estimation of cell viability by dye
29/08/25
exclusion (Trypan blue).
8 Aseptic culture techniques for
establishment and maintenance of 01/09/25
culture.
9 Preparation of stock solution of MS
basal media and plant growth regulator 22/09/25
stocks.
10 Micropropagation of tobacco plant leaf
06/10/25
disc culture.
11 Micropropagation of rice by indirect
13/10/25
organogenesis from embryo.
12 Preparation of fresh competent cell of
E. coli for harvesting plant 26/11/25
transformation vectors.
13 Transformation of competent cells of E.
29/11/25
coli with plant transformation vectors.
14 Small scale plasmid preparation from
19/11/25
E. coli.
15 Spotting. 29/11/25

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