World Journal of Pharmaceutical

Sanap et al.
Research
World Journal of Pharmaceutical Research
SJIF Impact Factor 8.453

Volume 13, Issue 21, 686-702. Research Article ISSN 2277–7105

TO PREPARE AND EVALUATE HERBAL TOOTH POWDER FROM

NATURAL HERBS
1*
Badrinath B. Sanap, 2Rutuja P. Khalpe, 3Sudarshan D. Ichche, 4Pallavi S. Patil,
5
Krushna R. Kale and 6Piraji S. Devkar

At. Chanegaon Tq. Badnapur Dist. Jalna, Maharashtra 431213, Jalna, Maharashtra, India.

ABSTRACT
Article Received on
07 September 2024, Denitrifies are the product which is used to maintain the Oral Hygiene
Revised on 28 Sept. 2024, such as Freshness of mouth and to avoid tooth decay. The oral hygiene
Accepted on 18 October 2024
can be maintained throughout the day by using various denitrifies
DOI: 10.20959/wjpr202421-34198
prepared by herbal and synthetic ingredients. This work was carried out
to prepare Tooth powder which can be used as a tool for proper oral
hygiene and to overcome the side effect of the conventional Tooth
power prepared by synthetic ingredients. the Tooth Powder was
prepared by using various herbal ingredients which possess the
*Corresponding Author antibacterial, antiseptic and cooling properties. Oral hygiene is an
Badrinath B. Sanap
important key to uphold good appearance, thought of an individual and
At. Chanegaon Tq.
gives confidence. The tooth consists of two parts, the crown, and the
Badnapur Dist. Jalna,
Maharashtra 431213, Jalna, root. The crown of the tooth is covered by an outer surface called
Maharashtra, India. enamel and it is the hardest tissue in the tooth. The major composition
of enamel is hydroxyapatite other than that it consists of water and
keratin. Dentine is the under part of the enamel, which is a composite of hydroxyl apatite. It
also consists of 70% of the collagen water. Fluorine is the major constituent of dentine. Oral
consists of not only tooth but also saliva for easy to consume the food. Saliva is the major
element proposed for lubricate the food and to maintain an appropriate environment in the
mouth. Saliva is formed by various glands such as Labial, lingual, buccal, and palatal are the
larger and smaller glands that produce saliva continuously to keep the tooth environment in
the dynamic state. Proteins, enzymes, bacteria, and mucous polysaccharide are there in the
saliva and inorganic materials like calcium, sodium, potassium, chloride, phosphate ions, etc.
The plaque, calculus, periodontal diseases are the major issues related to the tooth. It is
mainly caused by bacterial action and mineralized deposition leads to calculus. These diseases

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are mainly due to the negligence in good caring of the tooth, so it can be prevented and
controlled by proper brushing by using effective toothpaste and tooth powders.

KEYWORDS: Tooth Powder, Antibacterial, Dental, Herbal and Health.

INTRODUCTION
Dentifrice can be used as a prophylactic cosmetic for thetooth to avoid tooth decay and bad
breath. Dentifrice can be prepared by synthetic and herbal ingredients nowadays herbal
formulation is high in demand due to its effectiveness, to avoid the side effects when compared
with synthetic formulations. Toothpaste and tooth powders are based on its abrasive property,
the paste and powder apply on the tooth to rubagainst the tooth which helps to eliminate the
deposited food debris and minerals from tooth. "Oral health is essential to good health and
quality of life." Natural herbs are used in oral health products to treat various problems, such
as halitosis, bleeding gums, mouth ulcers, and dental caries. Herbs can be used either alone or
in combination. Some examplesof herbs used in oral hygiene products include.

Clove and cloveoil Neem and Neem oil

• Some studies have shown that herbs can prevent dental caries. For example, cinnamon
barkoil, Papua mace extract, and clove bud oil can inhibit the growth of many oral bacteria
• Herbs could have a potential beneficial effect in the dental field, such as with gum swelling,
specifically, and in oral healthcare overall. However, few studies have scientifically
reviewed these topics.
• In this chapter, the most common herbal supplements that can be used in dentistry (i.e.,
neem, ginger, clove, aloe vera, eucalyptus, garlic, miswak, turmeric, tulsi, charcoal, and
cinnamon) are reviewed. Evidence-based findings will be presented to support or refute
the use of these agents in oral care.
• Neem is scientifically known as Azadirachta indica and can be used as an endodontic
irrigation solution to minimize the E. faecalis, which is comparable to 3% of sodium
hypochlorite].
• It has antiviral, anti-bacterial (S. mutans, S. faecalis, S. salivarius, S. mitis, and S.
Sanghis), anti-sclerotic, and anti-inflammatory properties.
• Neem has been used in Ayurvedic traditions for centuries for agriculture, food storage,
andmedicine. It can help with:
• Cleaning: Neem can help reduce plaque buildup and prevent tartar stains
• Anti-inflammatory: Neem can help improve gum health

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• Antimicrobial: Neem can kill bacteria that cause gingivitis and pyorrhea, and can also
eliminate periodontal and tooth infections.
• Clove oil contains eugenol, which is an anesthetic and antibacterial agent that may help
with toothaches. Cloves and clove oil have been used as a home remedy for toothaches
sincethe 19th century.
• Neem and clove extract can be used for antimicrobial mouth washing, but more
preclinicaland clinical trials are needed to evaluate safety and biocompatibility Although
literature search reveals that Neem tree has multiple potential uses in dentistry, its
application is limitedin routine dental practice.

• AIM AND OBJECTIVE

A: - AIM
TO PREPARE AND EVALUATE HERBAL TOOTH POWDER FROM NATURAL HERBS.

B: - OBJECTIVE
• Promotes whitening of teeth
• Reduces cavities, tooth ache & gum bleeding
• Strengthens gums
• Promotes fresh breath
• Possesses natural antiseptic properties
• Eliminates bacteria causing bad breath
• Helps treating Gingivitis & sore throat
• Effective formulation for reducing dental hypersensitivity
• Protects teeth, reduces inflammation & prevents plaque formation

• PHARMACOGNOSTIC ACCOUNT OF NATURALHERBSA:- NEEM

Common Name: - Neem, Nimtree, kadulimb Botanical Name: - Azadiarachta indica
Family: - Meliaceae
Biological Source: - Neem consists of the fresh or dried leaves and seed oil of Azadirechta
indica.
Geographical Source: -some say neem is native to the whole Indian subcontinent; others
attribute it to dry forest areas throughout all of South and Southeast Asia, including Pakistan, Sri
Lanka, Thailand, Malaysia, and Indonesia.

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Macroscopic Characters
Colour: - Smooth and dark green
Odour: - typical
Taste: Taste: - Bitter

Chemical Composition: - The major phytochemicals present in Neem are glycoproteins,

triterpenes, limonoids, flavonoids, phenols, tannins, nimbins, saponins, catechins, azadirachtin
and gallic acid.

Uses: -Neem has antibacterial properties that help remove oral pathogens, prevent cavities, and
treat gum disease. Neem can also help with tooth decay, oral infections, bleeding, and sore
gums treating skin, teeth, and other health issues. Neem leaves, seeds, bark, stems, oil, and
phytochemicals have antibacterial, antiviral, antiparasitic, and antifungal properties.

• Clove
Synonyms: - Caryophyllum, Clove flower, Clove buds
Biological Source: - Clove consists of dried flower buds of Eugenia caryophyllus, family
Myrtaceae. It should contain not less than 7.0 per cent (w/w) of eugenol calculated on dried
basis.
Family: - Myrtaceae

Geographical Source: -indigenous to Amboyna and Molucca islands. It is now cultivated

chiefly in Zanzibar, Pemba, Penang, Madagascar, Caribbean islands, Sri Lanka and India. In
India, cloves are grown in Nilgiri, Tenkasi-hills and in Kanyakumari district of Tamil Nadu state.
It is also cultivated in Kottayam and Quilon districts of Kerala.

Macroscopic Characters: - Colour: - Crimson to dark brown. Odour: - Slightly aromatic

Taste: - Pungent and aromatic followed by numbness
Size: - About 10 to 17.5 mm in length, 4 mm in width, and 2 mm thick.
Shape: -Hypanthium is surmounted with 4 thick acute divergent sepals surrounded by
dorneshaped corolla. The corolla consists of unexpanded membranous petals with several
starnensand single stiff prominent style. Cloves are heavier than water.

Chemical constituents: - Clove contains about 15 to 20 per cent of volatile oil; 10 per cent
13per cent of tannin (Gallotannic acid), resin, chromone and eugenin. The volatile oil of the
drugcontains eugenol (about 70 to 90 per cent), eugend acetate, Caryophyllenes and small

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quantities of esters, ketones and alcohols.

Uses: - Clove is used as a dental analgesic, carminative, stimulant, flavouring agent, an
aromatic and antiseptic. It is also used in the preparation of cigarettes. The oil is used in
perfumery and also in the manufacture of vanillin. People commonly use clove for toothache,
pain during dental work, dental plaque, hangover, indigestion, and many other conditions,
butthere is no good scientific evidence to support these uses.

• ROCK SALT
Synonyms:- Halite, Saindhava lavana, Rock salt.
Biological Source:- Sendha namak, a type of salt, is formed when salt water from a sea or
lakeevaporates and leaves behind colourful crystals of sodium chloride.
Chemical constituents:- Sodium chloride, Zinc, iron, Potassium, Magnesium, Calcium.
Uses:- Rock salt improves digestion and is a natural way to relieve stomach [Link] also used to
cure stomach infection and aids in deforming as well.
It helps stabilise blood pressure by maintaining a balance of high and low blood pressure. Rock
salt provides the entire essential and greatly improves the body immune system.

• CINNAMON
Synonyms:- Amber, bay, beige, bister, brick, bronze, buff, chestn, dalchini
Biological source:- Cinnamon is the dried inner bark of the coppiced shoots Nees.,
belongingto of Cinnamomum zeylanicum
family:- Lauraceae
Chemical Composition:- cinnamaldehyde, cinnamate, cinnamic acid, and numerous
essentialoils
family:- Lauraceae
Uses:- Cinnamon oils, extracts, and their compounds may help prevent cavities, treat gum
disease, and fight fungal and bacterial infections. Thus, hygiene products containing
cinnamonmay have some uses for oral health and pain relief.

• MULETHI
Synonyms:- Madhuka, Yashtimadhuka, Yashtik, Madhuyashtika
Biological source:- Mulethi is a small perennial herb, commonly known as licorice, sweet
wood, or mulaithi, that is indigenous to Eurasia, northern Africa, and western Asia.[12] The
Glycyrrhiza genus is widely distributed worldwide and it consists of more than 30 species.

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Botanical name:- Glycyrrhiza glabra

Family:- Fabaceae

Chemical constituents:- Roots contain glycyrrhizin, asparagin, sugar, starch, acid resin, gum,
mucilage, phosphoric, sulfuric & malic acids. Bark contains a small quantity of tannins.

Uses:- The antimicrobial and anti-bacterial properties of Mulethi inhibit the growth of
bacteriawhich causes cavities and bad breath. Dried Mulethi powder can be used to brush our
teeth and mouthwash thereby reducing plaque and keeping the gums and teeth healthy and
strong.

• MINT
Synonyms:- Oleum mentha piperita, Colpermin, Mentha Oil.
Biological Source:- It is obtained from fresh flowering tops of the plants known as Mentha
piperita Linn.
Family:- Labiatae.

Chemical Constituents:- Peppermint oil contains chiefly menthol to the extent of 70 per cent.
Other important constituents of the peppermint oil are menthone, menthofuran, jasmone,
menthyl acetate.

Uses:- It stimulates saliva production. Saliva acts as a natural mouthwash, rinsing away
bacteria and any bits of food that might feed bacteria.

• BENEFITS OF NEEM AND CLOVE IN ORAL CARE

A: - BENEFITS OF NEEM
• Neem Toothpaste: This is one of the easiest ways in which you can include neem in
yourdaily dental routine. There are several kinds of toothpaste available in the market with
neem extract as their main ingredient. Brush your teeth daily with such toothpaste, for
healthier teeth and gums.

• Neem Powder: Grind the neem leaves to turn them into a fine powder. You can then
makea paste by mixing this powder with a teaspoon of baking soda and water. This natural
paste can be used to brush your teeth in order to attain bright and clean teeth.

• Neem Oil: Did you know neem oil has several antibacterial properties that can be used in

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order to help reduce the risk of cavities and inflammation of the gums? Just add a tiny dropof
neem oil to your toothpaste and gently brush your teeth & gums with the same every day.

• Neem Concoction: One can gargle their mouth with a neem concoction in order to fight
the build-up of bacteria in the mouth, thus preventing bad breath. To make this concoction,
th
just boil neem leaves in water and wait till the volume of the water is reduced to 1/4 .

• Neem Twigs: In ancient times, people used to brush their teeth with neem twigs. One
canfollow this old method and brush their teeth using neem twigs. This will help in
preventing gum diseases and will also work towards whitening the teeth.

• Neem Bark: Chewing on neem bark is also one such method that can be used to fight
various dental concerns such as cavities and gum diseases. Not only this but neem bark isalso
used in several tooth powders and pastes in order to ensure healthy oral care for people.

ROLE OF NEEM IN ORAL CARE

Neem as a preventive major in dental care the most effective usage of neem bark and
leafextract is to prevent cavities and gum disease.

Tooth decay, oral infections, bleeding, and painful gums can all be treated with neem
mouthwash has antibacterial properties that can help with gum disease, cavities, and plaque
buildup. Neem bark is used in many toothpastes and toothpowders, and neem twigs canbe
used as oral fresheners and toothache relievers.

B: - BENEFITS OF CLOVE
Cloves which is the scientific name of Syzygium aromaticum are used as an element in
Ayurvedic medicine.
In India, it is used as a spice material to add flavor to the food items. Similarly, theingredient is
used in Chinese and African cookeries, drinks, etc.
Do you know the dental care products like toothpaste, mouthwashes also contain cloves?
It is because the aromatic flower buds (cloves) accommodate germicidal properties whichare
required to get relief from various dental problems. A detailed note on the dental health
benefits of cloves has been given here.

• High nutritional value

Cloves have a high nutritional value which is comprised of:

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Calories, Fiber, Proteins, Calcium, Carbohydrates Manganese, Potassium, Vitamins A, C, E

and K These compounds present in cloves are effective to abolish the fungal infections which
areresponsible for various dental diseases.

• Provides relief from toothache

Clove oil – The fluid extracted from cloves are rich in a chemical compound called Eugenol.
This is rich in anesthetic, antibacterial and antibacterial properties. Thus the Eugenol provides
effective relief from pain in the oral regions. Likewise the clove oil is effective in soothing the
throbbing nerves in gums and alleviate gum infections, gum diseases like Periodontitis, etc.
Dentists also recommend clove oil as a natural remedyfor periodontal diseases.

• Highly efficient in killing bacteria

The antimicrobial properties and compounds present in cloves are powerful to avert the growth
of microorganisms inside our mouth. Cloves have the potent to execute Escherichiacoli (E.
coli) which is responsible for gum diseases, peri-implantitis and other harmful oral diseases.

Dental Health Specialists recommend rinsing the mouth with clove oil every day
willstrengthen the gums and remove plaque build-up over the gum surfaces.

• Protect bone health

Magnesium is the mineral which helps in bone development and enhances the bone volume.
The Eugenol and manganese present in the cloves safeguard the bones. As cloves are rich in
both these compounds, the spicy ingredient takes care of the bone developmentand feeds the
required nutrients to strengthen the bone.

When you fix implant teeth, a new bone needs to be developed around the implant.
Thisprocess is known as Osseointegration. Cloves are competent to aid the process of bone
formation.

• MATERIAL AND METHOD OF PREPRATION

• INGREDIENTS
Sr. Chemical Collection Of Raw
Ingredients Image
No. Constituents material
Nimbin, Nimbinene,
It is collected from Neem
1 Neem leaves Nimbandiol and
plant.
Nimbolide

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It is issue from our college

Eugenol and Volatile
2 Clove laboratorySSGMCOP
oil
Buldhana.

It was purchased from

3 Rock salt Sodium Chloride
Buldhana market.

It is issue from our college

Volatile oil, Tannin
4 Cinnamon laboratorySSGMCOP
and Mannitol
Buldhana.

It was purchased from

5 Mulethi Glycyrrizic acid
Buldhana market.

Menthol,
It is collected from Mint
6 Mint leaves Flavonoids, Resin
plant.
and cineole

• Method for the preparation of herbal toothpowder

• Take a morter & pastel in that adding the ingredient that are given below.
• For making this powder we take 8gm of rock salt and then grind it properly.
• Then we take 7gm of the clove powder after that,
• We are adding 4.78gm of cinnamon powder and then,
• Addition of 8gm of mulethi powder in the morter & pastel and then
• We are added dry neem leaves and dry mint leaves in the morter & pastel.
• After that we grind it properly into the morter & pastel.
• The fine powder is formed then we pass from the sieve then the powder is collect into
thesuitable container then label it properly.

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Table 1: Composition Of Herbal Toothpowder.

Sr. No Ingredients Quantity taken Role
1 Neem leaves 3.5 gm Antiseptic
2 Clove powder 7 gm Dental analgesic
3 Rock salt 8 gm Cleaning
4 Cinnamon powder 4.78 gm Analgesic
Antimicrobial and
5 Mulethi powder 8 gm
Antibacterial
6 Mint leaves 3 gm Antifungal

Table 2: Formulation table.

Sr. No. Ingredients F1TP F2TP
1. Neem leaves 3.5 gm 3.7 gm
2. Clove powder 7 gm 7.2 gm
3. Rock salt 8 gm 8.2 gm
4. Cinnamon powder 4.78 gm 4.80 gm
5. Mulethi powder 8 gm 8.2 gm
6. Mint leaves 3 gm 3.2 gm

6. BEBEFITS OF HERBAL TOOTH POWDER

Herbal tooth powder is an excellent way to improve your overall dental health. Here are some
of the benefits that Ayurvedic tooth powders can offer.

• Contains natural ingredients

Ayurvedic tooth powders are made with all-natural ingredients, making them a safe choicefor
dental care. Unlike chemical toothpaste, ayurvedic toothpowder ingredientsare not chemicals.
As such, it reduces your exposure to harmful chemicals that are often found in regular
toothpaste, such as fluoride or sodium lauryl sulphate.

• Strengthens Gums
The ingredients in ayurvedic tooth powders, such as Babul, Bakul, and Anantmul, are
knownto strengthen gums and help prevent gum disease. In particular, Babul bark powder has
been used for centuries as a natural tooth cleaner and gum-strengthening agent. It has anti-
inflammatory properties, and it can also prevent gum bleeding.

• Prevents tooth decay and cures different oral problems:

Ayurvedic tooth powders are also believed to prevent tooth decay and cure different oral
problems like pyorrhea, swollen gums, bleeding gums, and inflammation of the gums.

One of the known Ayurvedic tooth powders ingredients is Amla known for its high vitamin C

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content and its ability to kill bacteria that cause gum disease, cavities, and bad breath. It
alsohelps strengthen the teeth and prevent premature tooth decay.

In addition, herbal tooth powder benefits are useful for tackling bad breath and oral odor and
providing relief from toothaches. Moreover, several herbal ingredients included in
ViccoVajradanti tooth powder, like Anantmul, Amla, Bakul, and Lavang, are known for their
strong antimicrobial properties. As such, they help to fight off bacteria and other harmful
organismsthat cause gum inflammation, bad breath, and other oral health problems.

Oral health is integral to general wellbeing and relates to the quality of life that extends
beyond the functions of the craniofacial complex. Herbs have been used for centuries to
prevent and control dental disease. Herbal extracts are effective because they interact with
specific chemical receptors within the body.

Antimicrobial activities of these have been found to be particularly useful for periodontal
diseases. Aloe vera, Bloodroot, Caraway, Chamomile, Clove, Cranberry, Evening Primrose,
Garlic, Ginger, Green Tea, Haritaki, Liquorice, Myrrh, Neem, Peppermint, Propolis, Purple
Coneflower, Rosemary, Sage, Thyme, Turmeric, Tulsi and Triphala are useful in dentistry.
Table represents list of drugs used in oral care.

Herbal remedies have a long history of use for gum and tooth p In many traditional cultures,
there are no plastic-bristle brushes, rather, the use of herbal "chewing sticks" are common.

Chewing sticks are usually taken from plants, shrubs or trees with high anti-microbial activity.
The ends of selected sticks are shredded and they are used to massage the gumsand "floss"
the teeth.

In California, the young twigs of any of a number of oaks or willows have served for chewing
sticks, and are still available today.

In Traditional Medicine, the many herbs have a long history of use for prevention and in
assisting the body to heal itself in gum and tooth disorders.

Properties
• Using natural products.
• Rinsing & Flossing.

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• Monitoring your oral health.

• Building a routine.
• Regular visits to your dentist.

Disadvantages of tooth powder

-Some herbs might leave behind a texture on your teeth.
-may be too abrasive for teeth
-may be too abrasive for teeth

7. EVALUATION OF HEARBAL TOOTH POWDER

The prepared herbal tooth powder was evaluated for its various parameters such as
organoleptic, physic-chemical, rheological evaluation and anti-microbial activity.

• Organoleptic Evaluation
Organoleptic characteristics for various sensory characters like colour, odour, taste was
carefully noted down as illustrated. The raw drugs and powder were separately studied
byorganoleptic and morphological characters like colour, odour, texture, and appearance.

Colour: The prepared tooth powder was evaluated for its colour. The colour was checked
visually under normal lamp.

Odour: Odour was checked by smelling the product. Taste: Taste was manually checked by
tasting the product.

Table 4: Organoleptic evaluation of herbal tooth powder.

Sr. No. Parameters Result
1. Colour Yellowish green
2. Odour Characteristics
3. Taste Slightly bitter
4. Texture Fine
5. Appearance powder

• Physic-chemical Evaluation
-The physical and chemical feature of the herbal tooth powder was evaluated to determinethe
pH, its moisture content, and ash value and the amount of inorganic matter present in it.

• pH
-PH of formulated herbal tooth powder was observed by using pH meter. 5gm of tooth powder

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placed in 100ml of beaker. Allow the 10ml of boiled and then cool water. Stir vigorously to
make a suspension and measured the pH.

• Moisture Content
Tooth powder (10gm) weighed and dried it in the oven at 1050 C then it was cooled. Theloss
of weight is recorded as percentage moisture content and calculated by the given formula %
Moisture content = Original sample weight-Dry sample weight.

• Ash value
Weight accurately about 3gm of the powdered drug in silica crucible, Incinerate the
powdered drug by increasing the heat gradually until the sample was free from carbon
andcool it keep it in a desiccators. Weigh the ash and calculate the percentage of total ash in
contrast to the air dried sample.

Table 5: Physical-chemical evaluation.

Sr. No. Parameters Result
1. PH 5
2. Ash value 0.58
3. Moisture content 1.64

• Rheological Evaluation
Physical parameters like bulk density, foaming test, angle of repose ware observed
andcalculates for the formulation.

• Bulk Density
• Foam test
• Flow Property

• Bulk Density: -The bulk density of the powder is the ratio of the mass of an
untappedpowder sample and its volume including the contribution of the inter- particulate void
volume. It is expressed in gram/ml.

Bulk density = Untapped density - tapped density

• Foam test
The foamability of the product was evaluated by taking 2gms of tooth powder with water ina
measuring cylinder initial volume was noted as v1 and then shaken for ten times. Final volume

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of foam was noted v2.

• Flow Property
A funnel was taken and fixed with a clamp to the sand. A graph paper was kept below the
funnel and the height between graph paper and bottom of the funnel was measured.
Then50gm of powder was weighed and poured into funnel by blocking the orifice of the
funnel by thumb, the thumb was removed. The powder s2tarted flowing down onto the graph
paper and formed a cone shaped pile until the peak of pile become touched to the bottomof the
funnel stem. Then, the angle of repose was calculated by following formula. Tan 0 = H/R
H = Height of powder, R = Radius of graph paper

Table 6: Rheological evaluation of herbal tooth powder.

Sr. no. Parameters Result
1. Bulk density 4gm /ml
2. Foam Present
3. Angle of repose 48.74

• Anti-Bacterial Activity
Anti-bacterial activity was determined by agar well diffusion method-Preparation of Agar
media.
Suspended 7gm Nutrient agar in a 250ml conical flask and 250ml distilled water was added.
Then, it was heated on a hot plate with frequent agitation until it completely dissolved.

Then, the media was sterilized in an autoclave at 121°C for 1 hour. Agar well diffusion
method.

Approximately 25ml of Nutrient agar was poured into a sterile petridish and allowed to
solidify. 50µl of bacterial inoculums was spread on the solidify nutrient agar media by
usingthe sterile spreader. In these plates, four wells (5mm diameter) were punched into the
agarby using a sterile cork borer. Then, the working concentration of 100mg, 200mg, 300mg,
and 400mg dilution were prepared and was separately added into wells and allowed to diffuse
at room temperature. standard antibiotic (Erythromycin) was used as a positive control. The
plates were incubated for 24hours at 37°C for one day and another day I seen the zone of
inhibition.

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Table 7: Aati-microbial activity of E. coli.

Minimum inhibition
Culture Results
concentration
Escherichia coli 0.1mg/ml Sensitive

• RESULT
Sr. no. Evolution Result
1. Colour Yellowish green
2. Odour Characteristics
3. Taste Slightly Bitter
4. Texture Fine
5. Appearance Powder
6. pH 5
7. Ash value 0.58
8. Moisture content 1.64
9. Bulk density 4gm/ml
10. Foam Present
11. Angle of repose 48.74

• CONCLUSION
The research concluded that herbal tooth powder an emphasizing and more acceptable in
dental research and they are safer with minimum 3 side effect than synthetic preparation. The
formulated tooth powder capable to the tooth and oral hygiene and show the anti-microbial
activity against pathogens. The formulated herbal tooth powder has beengood scope in future
in nature remedies research and dental health of public. Natural plant products are an
important source to control bacterial pathogens. Therefore, in the present study, a herbal tooth
powder was developed and evaluated for antimicrobial activity which has shown excellent
results. The ingredients are used in the present work, was screened andselected to possess anti-
microbial effect and to maintain oral hygiene as it claimed by its results as effective tooth
Powder. It does not cause any harmful effects, instead, it imparts good freshness and away
from bad Odour. Oral hygiene can be maintained in a reliable, safe, and inexpensive way by
using herbal tooth powder.

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