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Hemocytometer Q&A: Pipettes & Cleaning Guide

The document provides detailed information on the differences between RBC and WBC pipettes, including their features and other uses. It also compares the improved Neubauer counting chamber to the old version, outlines cleaning procedures for pipettes, and describes the characteristics of an ideally charged chamber, as well as the implications of overcharging and undercharging. Overall, it serves as a practical guide for understanding and using hemocytometers.

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49 views2 pages

Hemocytometer Q&A: Pipettes & Cleaning Guide

The document provides detailed information on the differences between RBC and WBC pipettes, including their features and other uses. It also compares the improved Neubauer counting chamber to the old version, outlines cleaning procedures for pipettes, and describes the characteristics of an ideally charged chamber, as well as the implications of overcharging and undercharging. Overall, it serves as a practical guide for understanding and using hemocytometers.

Uploaded by

JadedYT
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

HEMOCYTOMETER QUESTION & ANSWERS FOR PRACTICAL RECORD

1. Draw and label RBC and WBC pipettes. Tabulate the differences between
them. Give other uses of it.

Differences between RBC and WBC pipettes


Differentiating RBC Pipette WBC Pipette
Features
Colour of the Red Bead White Bead
Bead
Colour of the Red White
Mouth piece
Size of the bulb Larger Smaller
Size of the Narrow capillary bore lumen- Wide capillary bore lumen-
capillary bore Slow speed pipette fast speed pipette
lumen
Markings 0.5, 1 and 101 0.5, 1 and 11
Dilution Can be done 100 or 200 times. Can be done 10 or 20 times.
(Dilution factor of 200 is practiced (Dilution factor of 20 is
in the physiology lab) practiced in the physiology
lab)
Other uses of Pipettes:
RBC Pipette: is used for
a. Total sperm count
b. Platelet count.
c. Conditions with high leukocyte count eg. Leukemia

WBC pipette: is used for


a. Cell count in CSF
b. Absolute eosinophil count,
c. RBC count in severe anaemia.

2. How does improved Neubauer’s chamber differ from old version

Neubauer counting chamber Improved Neubauer counting chamber


The total ruled area is 3mm X 3mm The total ruled area is 3mm X 3mm
The central ruled area is 1mm X 1mm The central ruled area is 1mm X 1mm
Central area consists of 16 groups of 16 Central area consists of 25 groups of 16 small
small squares separated by triple squares separated by triple rulings
rulings

3. How do you clean the pipettes and other components of haemocytometer?

• Firstly, the blood clots in the Pipette should be dissolved and removed by using
strong acid such as hydrogen peroxide.
• Then the pipette should be cleaned using distilled water.
• Pipette can be cleaned with alcohol for quick drying.

4. What are the features of an ideally charged chamber? When do you call it
over charged or under charged?
• An ideally charged chamber is completely covered with the diluted blood over the
counting grid under the cover slip and there should not be any air bubbles.
• Over charging refers to overflow of diluted blood in to the gutters- As the cells
enter the gutter and settle, over charging leads to false low cell count.
• Under charging refers to incomplete spread of the diluted blood in the central
platform. In such case, the cells will not be present in the peripheral areas leading
to false low cell count.

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