Multiple Choice Questions
1) Chemical Interferences are common than spectral interferences due to
a) Formation of compounds of low volatility
b) Ionization in Flames
c) Increase in rate of atomization
d)No shift in ionization equilibrium
2) Wave number is the number of wave
a) Per second
b) Per Centimetre
c) Per inch
d) Per centimetre 2
3) The colour which the human eye perceives is not the colour corresponding to the
wavelength of the light
a) Reflected
b) Absorbed
c) Refracted
d) Diffracted
4) Glass transition temperature is detected through
a) X-ray diffractometer
b) Solution calorimetry
c) Differential scanning calorimetry
d) Thermogravimetric analysis
5) One of the following materials is used for preparation of master grating. Identify
a) Glass
b) Iron
c) Aluminium
d) Teflon
6) A moiety of a molecule responsible for selective absorption of radiation in a
specific range is called as
a) Auxochrome
b) Catalyst
c) Chromophore
d) Elicitor
7) Beer’s law states that
a) Absorbance of a solution is indirectly proportional to the absorbing solute
b) Absorbance of a solution is indirectly proportional to the length of cell
c) Absorbance of a solution is directly proportional to the absorbing solute
d) Transmittance of a solution is directly proportional to the absorbance solvent
8) Extinction
a) Log(I0/It)
b) Log T
c) It/I0
d) I010-ct
9) How is the absorbance affected by the concentrations of a substance ?
a) It decreases
b) It remain constant
c) It increases
d) It varies randomly
10) The region of the electromagnetic spectrum below 200nm is known as
a) Vacuum ultra violet region
b) Far UV region
c) Low UV region3
d) Microwave region
11) The UV- visible region in the electromagnetic spectrum of radiation is
a) 200-400 nm
b) 300-660 nm
c) 400-800 nm
d) 200-800 nm
12) Energy absorbed in U.V region produces changes in
a) The rotational energy of the molecule
b) The vibrational energy of the molecule
c) The electronic energy of the molecule
d) All three energy levels of the molecule
13) Which amongst the following auxochrome produces a shift towards higher
energy wave length
a) -CH3
b) -NHCH3
c) -Cl
d) -C=O
14) Which source of light contains xenon gas at high pressure and exhibits greater
intensity compared to hydrogen discharge lamp
a) Hydrogen discharge lamp
b) Xenon discharge lamp
c) Deuterium lamp
d) Mercury lamp
15) Which of the following transition required least amount of energy
a) σ -σ*
b) n-σ*
c) n-π*
d) π-π*
16) Which of following the compound does not absorb light in the UV/ visible
spectrum
a) aspirin
b) paracetamol
c) Chloral hydrate
d) Phenobarbitone
17) Which of the following shift has highest energy and shorter wavelength
a) Bathochromic
b) Red shift
c) Conjugation shift
d) Hypsochromic shift
18) Solvent shift in the UV-VIS spectroscopy is related to
a) Boiling point
b) Polarity
c) Molecular mass
d) None of these
19) In IR spectrum, the functional group region is from
a) 4000cm-1 to 900cm-1
b) 4000cm-1 to 1400cm-1
c) 1400cm-1 to 900cm-1
d) 4000cm-1 to 660cm-1
20) Infra red spectroscopy is convenient method for understanding of
a) Drug receptor interaction
b) Functional group identifications
c) Physiochemical properties
d) Conformational properties
Long Questions
1. What are the principle involved in UV spectroscopy, explain in details about the
instrumentations, application of UV spectroscopy.
2. Describe the different sample preparation techniques used in IR spectroscopy.
Write down principle and a short note on different components of IR
spectroscopy.
5 Marks
1. Principle and application of flame photometry.
2. Define and distinguish between fluorescence and phosphorescence and derive
the phenomenon of fluorescence.
3. Derive Beer and Lambert’s law, what are deviations observed from Beer and
Lambert’s law.