MAHARANA PRATAP
(Approved by PCI, New Delhi and Affilia
Pradesh)Kothi, M
Following paper ID and Roll No. to be filled in your Answer Book)
ROLL NO.
B. PHARM
VII SEM., (SEC. J2) I MID SESSIONAL EXAMINATION, 2025-26
SUBJECT CODE: BP701T SUBJECT NAME: Instrumental Methods of Analysis
Time: 1hr. 30min. Total Marks: 30 M
Note: 1. Attempt all Sections. If require any missing data; then choose suitably.
SECTION A
1. Attempt all questions in brief. 5 X 2 M = 10 M
Q. No. Question Mark CO BL
s
a. What is the difference between normal phase and reveres phase 2 1 2
chromatography?
b. Define Chromophores and Auxochromes with suitable 2 1 2
example.
c. What do you understand by wet packaging and dry packaging? 2 2 3
d. Discuss the Bathochromic shift and Hypsochromic shift? 2 1 2
e. Write about electronic transition in UV Spectroscopy. 2 2 3
SECTION B
2. Attempt any one (01) of the following: 1 X 10 M= 10 M
Q. No. Question Marks CO BL
a. Enlist the different component of UV -Visible 10 1 2
spectrophotometer and explain the working of double beam
spectrophotometer along with labelled diagram.
b. Write about the principle, methods and applications of TLC? 10 2 3
SECTION C
3. Attempt any two (02) of the following: 2 X 5 M = 10 M
Q. No. Question Marks CO BL
a. Give about electrophoretic mobility. Explain factors affecting 5 2 3
electrophoretic mobility.
MAHARANA PRATAP
(Approved by PCI, New Delhi and Affilia
Pradesh)Kothi, M
b. Define and derive Beer lambert’s law. What is molar 5 1 2
absorptivity?
c. Write note on theory and working of gel electrophoresis. 5 2 3