91. Select the incorrect statement.

(a) Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.

(b) Insertional inactivation of β-galactosidase leads to colourless colonies.
(c) Insertional inactivation of β-galactosidase leads to blue color colonies.
(d) In insertional inactivation, the rDNA is inserted within the coding sequence of an enzyme
β-galactosidase.
92. Which vector is use to deliver gene in animal cell?
(a) Retroviruses (b) Disarmed retroviruses
(c) Ti-plasmid (d) E. coli
93. To make bacterium competent (Transformation with recombinant DNA) we use
(a) Specific concentration of Ca2+ ion (b) Heat shock (42°C)
(c) Both (a) and (b) (d) None of these
94. By which method is rDNA directly placed in nucleus of animal cell?
(a) Gene gun (biolistics) (b) Heat shock
(c) CaCl2 (d) Micro-injection
95. The way to introduce alien DNA into host cell includes
(a) Disarmed pathogens (b) Biolistics or gene gun
(c) Micro-injection (d) All of these
96. Which method is suitable for transferring an alien DNA into a plant cell?
(a) CaCl2 (b) Biolistics or gene gun
(c) Micro-infection (d) Heat shock
97. Arrange the following process in the sequence used in rDNA technology.
(1) Isolation of DNA
(2) Fragmentation of DNA by RE
(3) Isolation of desired DNA fragment
(4) Ligation of DNA fragment into vector
(5) Transferring rDNA into host
(6) Culturing host cells in a medium at large scale
(7) Extraction of the desired product
(a) 1, 2, 3, 4, 5, 6, 7 (b) 1, 2, 3, 4, 6, 5, 7
(c) 2, 1, 3, 4, 5, 6, 7 (d) 1, 2, 3, 5, 4, 6, 7
98. In majority of organisms the genetic material is
(a) DNA (b) RNA
(c) mRNA (d) Protein
99. Which match is correct for the enzyme which is used to extract DNA in following
organism?
(a) Plant cell–Cellulose (b) Fungus–Chitinase
(c) Bacteria–Lysozyme (d) All are correct
100. In isolation of genetic material (DNA) from nucleus, the RNA can be removed by
ribonuclease
and protein by protease. The purified DNA then precipitates after the addition of _________ .
(a) CaCl2 (b) Ether
(c) Chilled ethanol (d) Acetic acid
101. Which of the following is used to check the progression of restriction enzyme digestion?
(a) PCR (b) ROP
(c) AGE (d) All
102. PCR stands for
(a) Polymer chain reaction
(b) Polymerase chain reactor
(c) Polymerase chain reaction
(d) Polymerization, combination, recognition
103. 30 cycles of PCR amplified DNA approximately is how many times?
(a) 1 billion times (b) 1 million times
(c) 100 times (d) 1000 times
104. If any protein encoding gene is expressed in a _________ host, it is called a
_________
protein.
(a) Homologous host, recombinant (b) Heterologous host, recombinant
(c) Heterologous host, non-recombinant (d) Homologous host, non-recombinant
105. What is the maximum volume of culture that can be processed in bioreactors?
(a) 10–100 litres (b) 100–1000 litres
(c) 1–10 litres (d) 1000–1,00,000 litres
106. Which of the following is true about EFB defi nition of biotechnology?
(a) It includes traditional view.
(b) It includes modern molecular biotechnology.
(c) Defi nition is the integration of natural science and organisms, cells, parts thereof, and
molecular analogues for products and services.
(d) All the above
107. Which of the following can be biotechnological products?
(a) Antibiotics (b) Vaccine
(c) Enzymes (d) All of these
108. Select the false statement from the following.
(a) Genetic engineering includes techniques to alter chemistry of genetic material (DNA and
RNA).
(b) Sexual reproduction is more advantageous than asexual reproduction.
(c) Genetic engineering allows us to introduce desirable set of genes without the undesirable
gene into the target organism.
(d) Plasmid is autonomously replicating linear extra chromosomal DNA.
109. Multiplication of alien DNA in organism requires
(a) ROP (b) ORI
(c) Stop codon (d) TATA box
110. Select the incorrect matching.
(a) pBR322 − E. coli cloning vector
(b) EcoRI, Cla I, Hind III− Restriction enzyme
(c) ROP − Protein involved in the replication of the plasmid
(d) PCR − Technique in which multiple copies of the gene (or DNA) of interest is
synthesized in vitro
111. Plasmids are a suitable vector for gene cloning because
(a) They are small circular DNA capable of integrating with host genome.
(b) They have site for origin of replication.
(c) They can be used as a shuttle between prokaryotes and eukaryotes.
(d) They often carry antibiotic resistance genes.
112. PCR technique is considered as best for
(a) DNA synthesis (b) DNA amplification
(c) Protein synthesis (d) Amino acid synthesis
113. Gel electrophoresis is used for
(a) Construction of rDNA
(b) Isolation of DNA
(c) Cutting of DNA
(d) Separation of DNA fragments according to their size or length
114. Bacteria protects themselves from viral attack by producing
(a) Exonuclease (b) Endonuclease
(c) DNA ligase (d) Gyrase
115. Restriction endonuclease
(a) Cuts DNA molecule at random
(b) Cuts DNA from specific palindromic site
(c) Restricts DNA synthesis
(d) Synthesize DNA
116. Molecular scissor is
(a) Restriction endonuclease (b) Helicase
(c) Urease (d) Peptidase
117. Which one of the following is commonly used in the transfer of foreign DNA to crop
plants?
(a) Penicillium expansum (b) Trichoderma harzianum
(c) Meloidogyne incognita (d) Agrobacterium tumefaciens
118. DNA or RNA segment tagged with radioactive molecules is called
(a) Vector (b) Probe (c) Clone (d) Plasmid
119. Which of the following is a plasmid?
(a) pBR322 (b) BamHI (c) SalI (d) EcoRII
120. In the PCR technology, the DNA segment is replicated over a billion times. This
repeated
replication is catalyzed by the enzyme
(a) DNA polymerase (b) Taq polymerase
(c) DNA dependent RNA polymerase (d) Primase
121. An antibiotic resistance gene in a vector usually helps in the selection of
(a) Competent cells (b) Transformed cells
(c) Recombinant cells (d) None of these
122. Significance of the ‘heat shock’ method in bacterial transformation is to facilitate
(a) Binding of DNA to the cell wall.
(b) Uptake of DNA through membrane transport proteins.
(c) Uptake of DNA through transient pores in the bacterial cell wall.
(d) Expression of antibiotic resistance gene.
123. Which of the following is not a feature of the plasmids? [NEET - I, 2016]
(a) Independent replication (b) Circular structure
(c) Transferable (d) Single-stranded
124. The taq polymerase enzyme is obtained from: [NEET - I, 2016]
(a) Thermus aquaticus (b) Thiobacillus ferroxidans
(c) Bacillus subtilis (d) Pseudomonas putida
125. Which of the following is a restriction endonuclease? [NEET - I, 2016]
(a) Hind II (b) Protease
(c) DNase I (d) RNase
126. Stirred-tank bioreactors have been designed for [NEET - II, 2016]
(a) Addition of preservatives to the product
(b) Availability of oxygen throughout the process
(c) Ensuring anaerobic conditions in the culture vessel
(d) Purification of product
127. A foreign DNA and plasmid cut by the same restriction end nuclease can be joined to
form a
recombinant plasmid using [NEET - II, 2016]
(a) Taq polymerase (b) Polymerase III
(c) Ligase (d) Eco RI
128. Which of the following is not a component of downstream processing?
[NEET - II, 2016]
(a) Purification (b) Preservation
(c) Expression (d) Separation
128. Which of the following restriction enzymes produces blunt ends? [NEET - II, 2016]
(a) Eco RV (b) Xho I
(c) Hind III (d) Sal I
129. ‘Restriction’ in restriction enzyme refers to
(a) Cleaving of phosphodiester bond in DNA by the enzyme.
(b) Cutting of DNA at specific position only.
(c) Prevention of the multiplication of bacteriophage in bacteria.
(d) All of the above
130. Which of the following is not required in the preparation of recombinant DNA molecule?
(a) Restriction endonuclease (b) DNA ligase
(c) DNA fragments (d) E. coli
131. In agarose gel electrophoresis, DNA molecules are separated on the basis of their
(a) Charge only (b) Size only
(c) Charge to size ratio (d) Both charge and size
132. The most important feature in a plasmid to be used as a vector is
(a) Origin of replication (ori)
(b) Presence of a selectable marker
(c) Presence of sites for restriction endonuclease
(d) Its size
133. While isolating DNA from bacteria, which of the following enzymes is not used?
(a) Lysozyme (b) Ribonuclease (c) Deoxyribonuclease (d) Protease
134. Which of the following has popularized the PCR (Polymerase Chain Reactions)?
(a) Easy availability of DNA template
(b) Availability of synthetic primers
(c) Availability of cheap deoxyribonucleotides
(d) Availability of ‘thermostable’ DNA polymerase
135. An antibiotic resistance gene in a vector usually helps in the selection of
(a) Competent cells (b) Transformed cells
(c) Recombinant cells (d) None of the above