Scribd
Upload
9 views3 pages

MBBS Biochemistry OSPE Exam Key 2024

The document outlines the performance key for the OSPE practical examination for first-year MBBS students at Govt. Stanley Medical College, Chennai, in August 2024. It details various biochemical tests including procedures for testing calcium, chloride, phosphate, creatinine, urease, Molisch's test, and the Biuret test, along with the marks allocated for each step. Each test includes specific observations and interpretations of results, such as the formation of precipitates or color changes.

Uploaded by

5y9jhfv4kb
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
9 views3 pages

MBBS Biochemistry OSPE Exam Key 2024

The document outlines the performance key for the OSPE practical examination for first-year MBBS students at Govt. Stanley Medical College, Chennai, in August 2024. It details various biochemical tests including procedures for testing calcium, chloride, phosphate, creatinine, urease, Molisch's test, and the Biuret test, along with the marks allocated for each step. Each test includes specific observations and interpretations of results, such as the formation of precipitates or color changes.

Uploaded by

5y9jhfv4kb
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

DEPARTMENT OF BIOCHEMISTRY

GOVT. STANLEY MEDICAL COLLEGE, CHENNAI-1.


UNIVERSITY PRACTICAL EXAMINATION FIRST YEAR MBBS - AUGUST 2024

OSPE –PERFORMANCE KEY


[Link] PROCEDURE MARKS
1 TEST FOR CALCIUM
1
1) A pinch of Stone powder
2) 1ml of 4% 1
Ammonium Oxalate
1
3) Drops of concentrated ammonia
4) Drops of glacial acetic acid 1

5) Observation and interpretation 1

(White precipitate of calcium oxalate is observed)

2 TEST FOR CHLROIDE


1) To 3 ml of urine in a test tube 1
2) 1ml of concentrated nitric acid 1

3) 1 ml of silver nitrate solution.


1
4) Observation and interpretation 2

(White precipitate of silver chloride is observed)

3 TEST FOR PHOSPHATE


1
1) Pinch of Sample powder
1
2) 2ml concentrated HNO3
1
3) 2ml of ammonium molybdate
2
4) Observation and interpretation
(Canary yellow precipitate ammonium
phosphomolybdate is observed)
[Link] PROCEDURE MARKS
4 TEST FOR CREATININE
1) Take 5 ml of water in a test tube as control 1
2) Take 5 ml of test sample in a test tube as control 1
3) 1ml of saturated solution of picric acid 1
4) 10 drops of 10% NaOH. 1
5) Observation and interpretation 1
(Orange colour of creatinine picrate is formed)

5 SPECIFIC UREASE TEST


1) Take 5 ml of urine in a test tube & 5ml of 1
water in another test tube 1
2) Add 2 drops of phenol red indicator to each 1
tube
3) Add 2 drops of acetic acid till the solution
turns yellow
1
4) Add 2 ml of urease suspension to each of
these tubes
5) Observation and interpretation 1
(Pink colour is observed)

6 MOLISCH’S TEST
1) Take 5 ml of test solution in a test tube 1
2) Add 4 drops of Molisch’s reagent & mix 1
thoroughly
3) Incline the tube and allow 2 ml of 1
concentrated sulphuric acid to flow down the
side of the tube.
4) Observation and interpretation 2

( A reddish purple ring seen at the junction


of two liquids indicates presence of
carbohydrates-
Principle: Carbohydrates are dehydrated by
sulphuric acid to form furfurals which
condense with alpha naphthol to form the
violet/purple colored complex)

7 BIURET TEST
1) Take 3 ml of test solution in a test tube 1
2) Add equal volume of 5% NAOH 1
3) Mix well, then add 1% copper sulphate 1
solution drop by drop
mixing
4) Observation and interpretation
2
( Violet colour is observed)

You might also like