Blood Sample Collection Techniques Guide
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ScribdTranslationsBlood Sample Collection Techniques Guide
Translated by
ScribdTranslationsPractical class: Collection
Blood
Professor Tamires Lima
Caruaru - PE
Before the blood sample collection
Separate the tubes following the correct sequence.
Types of collection tubes
BLUE TAMPA
This tube contains the anticoagulant sodium citrate and is used for the
coagulation tests. The ratio of blood to anticoagulant should be
rigorously obeyed so as not to interfere with the test results.
YELLOW/RED FLAG
This tube contains clot activator to speed up the process of
coagulation and the separating gel for obtaining a good quality serum. It is
used in biochemistry routines, hormones, serology, immunology,
cardiac and tumor markers.
Types of collection tubes
PURPLE TAMPA
This tube contains the anticoagulant EDTA and is used for the routines of
hematology as the best anticoagulant for preservation of
cell morphology.
GREEN TAMPA
This tube contains heparin. It is used for biochemical analyses.
gasometry or other tests.
Types of collection tubes
GRAY TAMPER
This tube contains sodium fluoride anticoagulant and is used in the
glucose analyses.
WHITE TAMPA
This tube does not contain additive and is used for transport of
samples.
Before the blood sample collection
Place the label on the tube or identify the tubes with the data of
paciente: nome, número do registro, data de nascimento, sexo, data
from the collection.
Blood collection
The venipuncture technique can be performed in a closed (vacuum) or open (syringes) system.
Follow the steps for blood collection:
Clean your hands well;
Use personal protective equipment: lab coat, disposable gloves, goggles, and mask;
Ask the patient to state their full name for label confirmation.
Inform the patient about the procedure that will be performed;
Open the needle seal in front of the patient;
•Perform antisepsis of the area in a single direction from bottom to top or by changing the sides of the cotton or
from the center to the periphery (with circular movements);
Wait for the area to dry (do not blow or fan);
Strangle the patient's arm using this step for no more than 1 minute;
Make the puncture at a 30° angle with the bevel facing upwards;
When the blood starts to flow, release the patient's arm and ask them to open their hand;
Follow the correct sequence of collection tubes;
Blood collection
For vacuum collection, the phlebotomist should remove the tube when the blood stops flowing, having the...
make sure you collected the correct amount of blood. Because the vacuum blood collection tube
it has inside it the amount of vacuum proportional to the volume of blood that should be
collected for the type of tube. And this volume of blood collected is proportional to the amount of
anticoagulant/clot activator so that a quality sample can be obtained to be
analyzed
Gently homogenize the tubes by completely inverting them 5 to 10 times;
Place the tubes on a shelf in a vertical position to wait for the clot retraction (tube
of syrup/gel);
Dispose of the sharp material used in an appropriate container following the RDC.
no. 306/04;
Apply the bandage on the patient and instruct them to keep the collection site pressed for 3 to 5 minutes.
minutes and do not bend the arm to avoid bruises and bleeding;
If possible, prioritize sending the primary tube to avoid transferring tubes that
can generate patient exchanges;
Send the sample for processing.
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