CLUSTER UNIVERSITY SRINAGAR
SYLLABUS (FYUP UNDER NEP 2020)
Offered By Department of Chemistry
Semester 7th (Major -Course) CT-1
Course Title: Advanced Analytical Techniques
Course Code: UGCHM22J701 Max. Marks: 100
Credits: 4 (Theory: 3, Practical 1) Theory External: 60; Min Marks: 24
Contact Hrs: 75 ( Theory: 45, Practical: 30) Theory Internal (Continuous Assessment): 15 Marks, Min Marks: 06
Practical Experimental Basis = 15, Min. Marks: 06
Practical Experimental (Continuous assessment) = 10, Min. Marks: 04
Course Objectives
• To provide the student with an advanced understanding of the theory, practices and
instrumentation associated with advanced chromatographic separation techniques.
• To equip students with the knowledge and skills to understand and apply electrochemical
principles for chemical analysis.
Course Outcomes
On completion of the course, the student should be able to:
• Students will gain expertise in techniques like voltammetry, and coulometry for the
chemical composition and properties of the sample.
• Explain the fundamental principles and classifications of advanced chromatographic
techniques, including their theoretical and practical aspects.
Unit-I: Advanced Chromatographic Techniques (16 Contact Hours)
High-Performance Liquid Chromatography (HPLC): Principle, theory, instrumentation and
applications of HPLC.
Gas Chromatography-Mass Spectrometry (GC/MS): Principle, theory, instrumentation and
applications of GC/MS.
Size-exclusion/gel permeation Chromatography, Ion-exchange Chromatography, Affinity
Chromatography, Supercritical fluid chromatography
Unit-II: Electroanalytical Techniques (16 Contact Hours)
Voltammetry: General Introduction, Principle, Instrumentation, Linear & cyclic voltammetry, Normal and
differential pulse voltammetry, Squarewave voltammetry.
Coulometric analysis: Introduction, types of Coulometric Methods; Controlled Potential
Coulometry; Controlled Current Coulometry.
Unit III: Fluorescence Spectroscopy (16 Contact Hours)
Principles: Jablonski diagram, fluorescence, phosphorescence, intersystem crossing, fluorophores,
quantum yield, Stern-Volmer equation, steady-state fluorescence, fluorescence quenching, effect
of solvent on fluorescence quenching, Excitation and emission fluorescence spectra.
Instrumentation: Basic instrumentation in fluorescence
Applications: Application of fluorescence in sensing (with special reference to quantum dots and
organic fluorophores) in understanding the structural/conformational aspects of proteins.
Practicals (1 Credits- 30 Hrs)
1. Separation of organic mixture by Thin Layer Chromatography and identification of
functional groups using specific visualizing reagents.
2. Separation and Identification of binary organic mixture by Column chromatography.
3. Determine the concentration of quinine in the tonic water sample by using fluorescence
spectrophotometer.
4. To study the redox behavior of a given electroactive species using cyclic voltammetry and
determine parameters such as:
a) Formal potential (E°′)
b) Peak current (Ip)
c) Peak potential separation (ΔEp)
d) Reversibility of the redox couple
Suggested Books:
1. Quantitative Chemical Analysis by Daniel C. Harris and Fundamentals of Analytical
Chemistry by Skoog, West, and Holler.
2. Bard and Larry R. Faulkner, Electrochemical Methods: Fundamentals and Applications,
New York: Wiley, 2001, 2nd ed. Published: December 2002.
3. Principles and Practice of Modern Chromatographic Methods By Kevin Robards, Danielle
Ryan · 2021
4. Gary. D. Christian, Analytical Chemistry; Wiley Publication, 5th Edition, 2001.
5. Principals of Nanoscience and Nanotechnology, M. A. Shah and Tokeer Ahmad, Narosa
Publications, 2010.
6. Fluorescence Spectroscopy, by J. R. Lakowiz, Springer, (2006).
7. Molecular Fluorescence - Principles and Applications by B. Valeur, Wiley-VCH, (2002).