ABO Blood Group Inverse Test Guide
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ScribdTranslationsABO Blood Group Inverse Test Guide
Translated by
ScribdTranslationsInverse Test
(Test for ABO Groups)
Introduction
In the human species, there are several different genes involved in compatibility or
incompatibility of blood transfusions. The two main groups are known as
ABO and RH.
The ABO system is a consequence of the presence of a cover.
of protein in the red blood cell membrane. This protein is known in two ways
immunologically different, form A and form B refers to the type of proteins that
present the red blood cells; in the event that this protein is not present, these are included
individuals with blood type O.
The Rh factor corresponds to a plasma protein. The presentation of this protein is Rh+ and the
Rh- absence
The determination of blood type is carried out by confronting the problematic red blood cells with
reactives indicates the presence of the corresponding antigens
Foundation
The patient's serum that contains antigens comes into contact with the criteria with which
erythrocytes that contain antibodies forming an Ag-Ab complex, which manifests
microscopically through agglutination.
Test for ABO groups
Material:
Equipment for venipuncture
Excavated glass plate
Wooden applicators
Washed erythrocytes A and B known
Patient's serum
Procedure:
Red blood cell suspensions at 2% are prepared in the following
In a test tube marked with the letter A, 1 drop of blood A is placed, and another marked with
Letter B, a drop of blood is placed B, the tubes are filled with saline. And they are centrifuged at 3400.
rpm for 3 minutes and the supernatant liquid is decanted by inverting the tubes.
2. To each of the tubes, 1 cm of saline solution is added and the red blood cells are resuspended.
3. Two separate drops of problem serum are placed on a glass slide, to the left drop
1 drop of the erythrocyte suspension A is added to the left and 1 drop of the suspension to the right.
red blood cells B.
Interpretation of Results:
a) If the serum agglutinates A red blood cells ---- it has Ac A, the serum that has Ac A corresponds t
blood type B, the serum problem, corresponds to blood of type B.
b) If the serum problem agglutinates B erythrocytes has
----Ac B, the serum that has Ac B corresponds
the serum problem corresponds to a blood type A.
c) If the serum problem agglutinates erythrocytes A and B ---- it has antibodies A and B, the serum that has antibodies A and
B corresponds to blood type O, therefore the problematic serum corresponds to a blood type group.
O.
d) If the serum problem does not agglutinate in the red blood cells---A andit has
B neither Ac nor A nor B, so
Both the serum problem belongs to a blood type of group AB.
Results
Nombre: Yetzabeth Ángeles Cruz
Edad: 17 años
Sexo: Female
Grupo Sanguíneo: O
Conclusions
This test is widely used in Blood Banks, as it undoubtedly corroborates
the results of a blood typing done on a plate. Its accuracy makes it that the
blood in case of a transfusion is reliable, thus avoiding possible reactions or
rejection.