ANTIANGIOGENIC PROPERTIES OF BANANA (MUSA ACUMINATA) PEEL EXTRACTS
IN PARTIALLY OPENED CHICK EMBRYO
A research study presented in
SAMAR STATE UNIVERSITY
Catbalogan City
Province of Samar
In Partial Fulfillment
of the Requirements for the Degree
Bachelor of Science in Pharmacy
ABBANG, LIBBY JOY
CABANGUNAY, CARL DION
JABOLI, JUN ELBERT
NUBE, FRANCO
TADEOS, DENISE NADYN
September 2025
ABSTRACT
This study aimed to determine the antiangiogenic effect of different concentrations
of banana (Musa acuminata) peel extracts in aqueous solution on the chorioallantoic
membrane of partially opened fertilized chicken eggs. The study tested the hypothesis on
whether aqueous solutions of the peel extracts, at different dosages, could significantly
reduce the growth of blood vessels in partially opened chick embryos in terms of
branching and segment formation, illuminating its potential as an antiangiogenic agent in
cancer therapy.
Twenty-four fertilized chicken eggs were categorized and equally divided into four
groups, which includes one positive control group, one negative control group, and two
experimental groups. The positive control group was treated with 10mcg/mL of retinoic
acid, the negative control group was left untreated, and the experimental group A was
treated with 100mcg/mL of banana peel extract, and the experimental group B was
treated with 150 mcg/mL of banana peel extract. The results of the treatments were
initially gathered through imageJ software’s angiogenesis analyzer, and the data
pertaining to the number of blood vessel branching and segment development before and
after treatment underwent statistical treatment using the Mix Model Analysis of Variance,
followed by the Tukey’s Honestly Significant Difference (Tukey’s HSD) test. Key
parameters measured in this study were the number of branches and segments located at
the opening of the egg, before and after administration of the extract solutions.
A significantly lower increase in blood vessel development, in terms of branches and
segments, were observed in both of the experimental set-ups compared to the negative
control. The data shows that the experimental set-up B exhibited the second-least growth
in blood vessels on average (24.83 growth in branches, 65.33 growth in segments)
compared to the experimental set up A (26.17 growth in branches, 107.00 growth in
segments). However, as expected from the positive control, it showed the least
development in blood vessels in terms of branches and segments on average (15.83
growth in branches, 12.00 growth in segments), while the negative control showed the
greatest blood vessel development on average (54.17 branches, 147.84 segments) among
all four groups. Results have shown that banana peel extracts have comparable
antiangiogenic effects to known antiangiogenic substances such as retinoic acid, and it
shows the extract’s inhibiting property against blood vessel formation, as shown in its
comparison to the eggs which were left untreated. Statistical analysis of the data gathered
from the experiment confirmed the antiangiogenic effects of banana (Musa acuminata)
peel extracts, with higher doses showing a stronger antiangiogenic effect.
Banana (Musa acuminata) peel extracts have exhibited significant antiangiogenic
effects, particularly at higher doses, suggesting its potential as a natural antiangiogenic
agent. Further studies, with bigger sample sizes, are warranted to illuminate its potential
therapeutic use in cancer therapy.