Aim: Introduction to Neubauer Counting Chamber
Introduction
The Neubauer Counting Chamber, also called a haemocytometer, is a specially
designed thick glass slide used to count microscopic blood cells such as RBCs, WBCs,
platelets, and even microorganisms. It allows the determination of the number of cells
per cubic millimetre (cells/mm³) of blood by counting cells in a precisely known area and
volume.
It was originally developed by Louis-Charles Malassez and later modified by Neubauer,
which is why it is widely known as the Improved Neubauer chamber.
STRUCTURE OF THE CHAMBER
WHY WE USE THE NEUBAUER CHAMBER
1. Accurate and standardized cell counting method
2. Allows visual identification of individual cells
3. Inexpensive and does not require automation
4. Useful in:
o Hematological investigations
o White blood cell counts
o Red blood cell counts
o Platelet counts
o Sperm count
o Microbial cell counts
Principle
A diluted blood sample is spread on the counting grid.
Cells settle and occupy the space directly above the grid lines.
Because the dimensions (area + depth) are precisely known, the exact volume of the
sample counted is known.
By counting the cells within the defined squares and applying the dilution factor, the
concentration of cells in the original blood sample is calculated.
ADVANTAGES
• Simple, inexpensive
• Highly accurate when used correctly
• No complex equipment required
• Can count different types of cells using different diluting fluids
LIMITATIONS
• Requires careful handling
• Air bubbles or overfilling cause errors
• Needs proper mixing of blood sample
• Manual counting → operator-dependent