Prepared for the Foundation for Innovative New Diagnostics

MGIT TM

Procedure Manual

For BACTEC™ MGIT 960™ TB System

(Also applicable for Manual MGIT)
Mycobacteria Growth Indicator Tube (MGIT)
Culture and Drug Susceptibility Demonstration Projects

by

Salman H. Siddiqi, Ph.D.

(BD Fellow, Sparks, Maryland, USA )
and
Sabine Rüsch-Gerdes, Ph.D.
(Director, National Reference Center for
Mycobacteria, Borstel, Germany)

July 2006
Section IV: References

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7
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8
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13
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laboratory ready? J Clin Microbiol. 1993;31:767-770.

MGITTM Procedure Manual

 Section IV: References

14
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17
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18
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19
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20
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21
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22
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23
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24
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2000;19:750-754.

MGIT Procedure Manual

 Section IV: References

25
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26
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27
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28
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29
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30
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31
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the recovery of mycobacteria. Diagn Microbiol Infect Dis. 2000;37:31-36.
32
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33
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34
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35
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36
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Diagn Microbiol Infect Dis. 2002;44:157-161.

MGIT Procedure Manual

 Section IV: References

37
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mycobacteria from clinical specimens: Multi-Center study. J Clin Microbiol. 1999;37:3578-
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38
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39
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460 for culture of Mycobacteria. Diagn Microbiol Infect Dis. 2000;38:123-126.
40
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Pont Isolator for detection of mycobacteria. J Clin Microbiol. 1988;26:1501-1505.
41
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42
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Indicator Tube (MGIT 960) automated system for drug susceptibility testing of Mycobacterium
tuberculosis. J Clin Microbiol. 2001;39:4440-4444.
43
Adjers-Koskela K, Katila ML. Susceptibility testing with the manual Mycobacteria Growth
Indicator Tube MGIT) and the MGIT 960 System provides rapid and reliable verification of
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44
Bemer P, Palicova F, Rusch-Gerdes S, et al. Multi-center evaluation of fully automated
BACTEC Mycobacteria Growth Indicator Tube 960 system for susceptibility testing of
Mycobacterium tuberculosis. J Clin Microbiol. 2002;40:150-154.
45
Bergmann JS, Fish G, Woods GL. Evaluation of BBL MGIT (Mycobacteria Growth Indicator
Tube) AST SIRE System for antimycobacterial susceptibility testing of Mycobacterium
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46
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47
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Mycobacterium tuberculosis to isoniazid and rifampin. Diagn Microbiol Infect Dis.
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48
Cambau E, Truffot-Pernot C, Boulahbal F, et al. Mycobacterial Growth Indicator Tube versus
the proportion method on Lowenstein-Jensen medium for antibiotic susceptibility testing of
Mycobacterium tuberculosis. Eur J Clin Microbiol Infect Dis. 2000;19:938-942.

MGIT Procedure Manual

 Section IV: References

49
Huang TS, Tu HZ, Lee SS, et al. Antimicrobial susceptibility testing of Mycobacterium
tuberculosis to first-line drugs: comparisons of the MGIT 960 and BACTEC 460 Systems. Ann
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50
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susceptibility testing of Mycobacterium tuberculosis to isoniazid and rifampin. Diagn Microbiol
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51
Tortoli E, Benedetti M, Fontanelli A, et al. Evaluation of automated BACTEC MGIT 960
system for testing susceptibility of Mycobacterium tuberculosis to four major anti-tuberculous
drugs: comparison with the radiometric BACTEC 460TB method and the agar plate method of
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52
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53
Aono A, Hirano K, Hamasaki S, et al. Evaluation of BACTEC MGIT 960 medium for
susceptibility testing of Mycobacterium tuberculosis to pyrazinamide (PZA): compared with the
results of pyrazinamidase assay and Kyokuto PZA test. Diagn Microbiol Infect Dis.
2002;44:347-352.
54
Pfyffer GE, Palicova F, Rusch-Gerdes S. Testing of susceptibility of Mycobacterium
tuberculosis to pyrazinamide with the non-radiometric BACTEC MGIT 960 system. J Clin
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55
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56
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57
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58
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59
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4170.

MGIT Procedure Manual

 Section IV: References

60
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66
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67
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68
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69
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70
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MGIT Procedure Manual

 Section IV: References

71
Hasegawa N, Miura T, Yamaguchi K, et al. New simple and rapid test for culture
confirmation of Mycobacterium tuberculosis complex: a multi-center study. J Clin Microbiol.
2002;40:908-912.
72
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specimens using the mycobacteria growth indicator tube (MGIT) and the Lowenstein-Jensen
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73
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74
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75
Kruuner A, Yates MD, Drobniewski FA. Evaluation of MGIT 960-based antimicrobial testing
and determination of critical concentrations of first- and second-line antimicrobial drugs with
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76
Rusch-Gerdes S, Pfyffer GE, Casal M, et al. Multi-center laboratory validation of the
BACTEC MGIT 960 technique for testing susceptibilities of Mycobacterium tuberculosis to
classical second-line drugs and newer antimicrobials. J Clin Microbiol. 2006;44:688-692.

MGIT Procedure Manual