1

Kill only bacteria

On surface of pathogen
Enyme
Produced by white blood cells

agenoyerm mphocy
Pr
.

response

Microorgnisms such as bacteria, fungi , virus

3
No only active immunity
No only active immunity
Yes both
No only active immunity
Passive immunity Active immunity
- is the transfer of • involves immune

antibodies resposnse
• Have memory cells
-no immune resposne
• Provide. Long term
-like mother to her fetus
immunity
through placenta
• Acquired b vaccination
-short term immunity with
no memory cells
4
X
-
-
-

Definition of antibiotics
• chemil susbstance produced by
microorogansims mainly fungi
• To kill or stop the growth of bacteria
 Cambridge IGCSE™

COMBINED SCIENCE 0653/21

Paper 2 Multiple Choice (Extended) May/June 2025
45 minutes

You must answer on the multiple choice answer sheet.

*0461813728*

You will need: Multiple choice answer sheet

Soft clean eraser
Soft pencil (type B or HB is recommended)

INSTRUCTIONS
 There are forty questions on this paper. Answer all questions.
 For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
 Follow the instructions on the multiple choice answer sheet.
 Write in soft pencil.
 Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
 Do not use correction fluid.
 Do not write on any bar codes.
 You may use a calculator.
 Take the weight of 1.0 kg to be 9.8 N (acceleration of free fall = 9.8 m / s2).

INFORMATION
 The total mark for this paper is 40.
 Each correct answer will score one mark.
 Any rough working should be done on this question paper.
 The Periodic Table is printed in the question paper.

This document has 16 pages. Any blank pages are indicated.

IB25 06_0653_21/2RP
© UCLES 2025 [Turn over
 2

1 Osmosis can be defined as the net movement of ......1...... molecules from a region of ......2......
water potential to a region of ......3...... water potential, through a partially permeable membrane.

Direct Which words complete gaps 1, 2 and 3?

1 2 3

A sugar higher lower

B sugar lower higher
C water lower higher
D water higher lower

2 The diagrams show four root hair cells.

Direct
Each cell is surrounded by soil with the same volume of water present.

Which root hair cell absorbs water the most rapidly?

A B

soil temperature 10 °C soil temperature 10 °C

C D

Temperture increse kinetic energy

= larger surface area
soil temperature 20 °C soil temperature 20 °C

3 Which statement explains how an increase in pH affects enzyme activity?

A The active site changes shape.

emp m
B ↑
The substrate is denatured.
C There is an increase in the kinetic energy.
D The number of collisions increases.

© UCLES 2025 0653/21/M/J/25

 3

4 What is the balanced equation for photosynthesis?

A CO2 + H2O  C6H12O6 + O2

B CO2 + 6H2O  6C6H12O6 + O2

C 6CO2 + 6H2O  C6H12O6 + 6O2

D 6CO2 + 12H2O  6C6H12O6 + 6O2

5 The diagram shows a transverse section through a leaf.

layer 1 Waxy cuiticle

layer 2 Upper epidermis

layer 3 Palisade mesophyll

layer 4 Spongy mesophyll layer

Guard cells
Which layers have cells that can produce oxygen in the presence of light? Carry photosynthesis

A 1, 2, 3 and 4
B 1, 2 and 3 only
C 2 and 3 only
D 3 and 4 only

6 What is the purpose of physical digestion? Mechnical ( no chemical chnge )

A break down food into smaller pieces • teeth
• Tongue

*
B break down insoluble food into soluble molecules

• Stomach churning
C change food so that it can be absorbed into the blood
D digest insoluble food molecules using enzymes
• Emulsification by bile juice

© UCLES 2025 0653/21/M/J/25 [Turn over

 4

7 The diagram shows the human alimentary canal and other organs.

Which label shows where glycerol is absorbed?

A Stomach ( Gastric juice with

protease )
B: pancreas relese pancreatic
juice ( APL )
C: small intetsine site of
A
absorption of food into blood B

D: large intestine absorb water

from faeces
C

8 Which row describes aerobic respiration?

C6H12O6 + 6O2……….6CO2+6H2O
nutrient molecules
produces water releases energy
broken down

A key
B = true
C = false
D

9 Which row about the anther and stigma of a wind-pollinated flower is correct?

anther stigma stigma

position position description

A inside flower inside flower smooth

B outside flower outside flower feathery
C outside flower inside flower smooth
D inside flower outside flower feathery

© UCLES 2025 0653/21/M/J/25

 5

10 Which row shows the effect of deforestation on oxygen and carbon dioxide concentrations in the
atmosphere?

oxygen carbon dioxide Less photosynthesis

Less oxygen
concentration concentration

More CO2
A decreases increases
B decreases no change
C increases decreases
D no change increases

11 Which term describes an area that contains all the organisms and their environment interacting
together?

A community Different species living. Particular area

B ecosystem
C food chain Energy flow Popultion : number of
D food web More thn one food chain individuls of same species
living in. Particular area
12 Which row shows how active immunity is gained?
Involves immune respsone
from antibodies after an
supplied in by vaccination infection by
an injection a pathogen

A key
B = yes
C = no
D

13 Which statements about antibiotics are correct?

1 -
An antibiotic is a drug.
2 X
Antibiotics kill bacteria and viruses.
3 MRSA is a type of bacteria resistant to antibiotics.
-
A 1 and 2 B 1 and 3 C 2 and 3 D 3 only

© UCLES 2025 0653/21/M/J/25 [Turn over

 Cambridge IGCSE™

COMBINED SCIENCE 0653/22

Paper 2 Multiple Choice (Extended) May/June 2025
45 minutes

You must answer on the multiple choice answer sheet.

*1293011556*

You will need: Multiple choice answer sheet

Soft clean eraser
Soft pencil (type B or HB is recommended)

INSTRUCTIONS
 There are forty questions on this paper. Answer all questions.
 For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
 Follow the instructions on the multiple choice answer sheet.
 Write in soft pencil.
 Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
 Do not use correction fluid.
 Do not write on any bar codes.
 You may use a calculator.
 Take the weight of 1.0 kg to be 9.8 N (acceleration of free fall = 9.8 m / s2).

INFORMATION
 The total mark for this paper is 40.
 Each correct answer will score one mark.
 Any rough working should be done on this question paper.
 The Periodic Table is printed in the question paper.

This document has 16 pages. Any blank pages are indicated.

IB25 06_0653_22/2RP
© UCLES 2025 [Turn over
 2

1 Movement is a characteristic of all living organisms.

Which two other characteristics of living organisms provide the energy for movement?

A excretion and nutritionExcretion ; Removal of waste products

B growth and sensitivity
C nutrition and respiration
D respiration and growth

2 Which plant cell structure matches its function?

structure function

A X Wall
cell membrane provides strength and support
B chloroplast absorbs light energy
C ribosome X protectionSite
provides of protein synthesis
D
X
vacuole site of chemical reactions
Cytoplasm
3 Which row describes osmosis?

osmosis is the net movement of water molecules

through a partially permeable membrane
from to
A a dilute solution with a concentrated solution with
a higher water potential a lower water potential
B a concentrated solution with a dilute solution with
a higher water potential a lower water potential
C a dilute solution with a concentrated solution with
a lower water potential a higher water potential
D a concentrated solution with a dilute solution with
a lower water potential a higher water potential

© UCLES 2025 0653/22/M/J/25

 3

4 The table shows the results of tests carried out on different food samples.

ethanol
food Benedict’s
emulsion biuret test
sample solution test
test

1 key
2 = positive result
3 = negative result
4

Which food samples contain reducing sugar?

A 1 and 2 B 1 and 3 C 2 and 4 D 3 and 4

5 Which statement describes enzyme action in humans?

A Enzymes become denatured at 0 °C. Deactivated

B X increases with an increasing temperature.
The activity of enzymes always
C X shape.
The enzyme active site and its substrate are the same complementary
D There are more-frequent effective collisions when the temperature increases from 20 °C to
30 °C.

6 Four tubes that contain red hydrogencarbonate indicator are set up as shown. After five hours,
the colour of the hydrogencarbonate indicator is recorded.

aquatic plant

hydrogencarbonate
indicator
in light in light in dark in dark
1 2 3 4

Which row shows the colour of the hydrogencarbonate indicator in each tube after five hours?

1 2 3 4

A purple red red red Indicator

B purple red yellow red
Yellow Red Purple
C red yellow red purple
Resp>photo
D yellow red purple red Photo>respiration

© UCLES 2025 0653/22/M/J/25 [Turn over

 4

7 A diet provides the correct energy requirements for a person but is not balanced.

What are possible effects of this diet?

1 constipation
2 obesity X
3 scurvy

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

8 What is a function of hydrochloric acid in the stomach? • Energ in put> out

A to help absorption of all food in the stomach
Gain weight
B to kill microorganisms in the ingested food
- Energy out put >in put
C to prevent chemical digestion
D to prevent the stomach contents being too acidic
Lose weight

9 Which statement explains why root hair cells have a long, thin shape?

A They are squeezed into this shape as they grow between soil particles.
B X such as glucose.
It is the most efficient shape for taking up food
C It provides a large surface area for diffusion of water.
D
X
The narrow diameter allows them to gain carbon dioxide from between soil particles.

10 Which row describes viruses?

are have a contain

pathogens protein coat genetic material

A
B
C
D

© UCLES 2025 0653/22/M/J/25

 5

11 The graphs show how the rate and depth of breathing varies when a student does four different
activities.

Which graph shows when the student is most active?

A B

volume of volume of
air in lungs air in lungs

0 0
0 10 20 0 10 20
time / s time / s

C D

volume of volume of
air in lungs air in lungs

0 0
0 10 20 0 10 20
time / s time / s

12 Which statement about the use of antibiotics is correct?

Multiresistant S aeurius
A Overuse of antibiotics causes the development of bacteria such as MRSA.
B X and bacteria.
Antibiotics are prescribed for infections caused by viruses
C
X
Antibiotics increase reproduction of non-resistant bacteria.
D X
Antibiotics only kill resistant bacteria.

© UCLES 2025 0653/22/M/J/25 [Turn over

 6

13 The diagram shows a food web.

eagle
fox

small bird

goat

ant
caterpillar

locust beetle

grass wildflower tree

Which row states the number of species of each category in this food web?

number of primary number of secondary number of tertiary

consumer species consumer species consumer species

A 6 1 1
B 5 2 1
C 5 2 2
D 3 4 3

© UCLES 2025 0653/22/M/J/25

 Cambridge IGCSE™

COMBINED SCIENCE 0653/23

Paper 2 Multiple Choice (Extended) May/June 2025
45 minutes

You must answer on the multiple choice answer sheet.

*4924602234*

You will need: Multiple choice answer sheet

Soft clean eraser
Soft pencil (type B or HB is recommended)

INSTRUCTIONS
 There are forty questions on this paper. Answer all questions.
 For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
 Follow the instructions on the multiple choice answer sheet.
 Write in soft pencil.
 Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
 Do not use correction fluid.
 Do not write on any bar codes.
 You may use a calculator.
 Take the weight of 1.0 kg to be 9.8 N (acceleration of free fall = 9.8 m / s2).

INFORMATION
 The total mark for this paper is 40.
 Each correct answer will score one mark.
 Any rough working should be done on this question paper.
 The Periodic Table is printed in the question paper.

This document has 16 pages.

IB25 06_0653_23/2RP
© UCLES 2025 [Turn over
 2

1 Which structure is not found in animal cells?

A cell wall
B cytoplasm
C nucleus
D cell membrane

2 Palisade mesophyll cells are specialised cells.

What is the function of these specialised cells?

A photosynthesis

Xylem ( water and minerals ) , phloem ( surose and amino acids )

B transport
C reproduction
D immunity

3 A human egg cell is 0.12 mm in diameter.

How is this converted to a measurement in micrometres, m?

A multiply by 100
B multiply by 1000 1 cm = 10 mm
C divide by 100
1 mm= 1000 um
D divide by 1000
1 um = 1000 nm
4 Which row about osmosis is correct?

molecules details of type of permeable

that move movement membrane
A water from a dilute solution partially
to a concentrated solution
B water from a concentrated solution fully
to a dilute solution
C solute from a dilute solution partially
to a concentrated solution
D solute from a concentrated solution fully
to a dilute solution

© UCLES 2025 0653/23/M/J/25

 3

5 The graph shows the activity of an enzyme at different temperatures.

enzyme
Has higher kinetic energy
activity
X Z
W
↓
native
comple
0 20 40 60
temperature / °C

Which statement about the effect of temperature on the activity of this enzyme is correct?

A At X and Z, the activity of the enzyme is the same because the kinetic energy of the enzymes
is the same.
B At W, the enzyme activity is lower than at X because there are less-frequent effective
collisions between the enzyme and the substrate.
C
X
At Y, the enzyme is working at its optimum rate because it has the most kinetic energy.
D At Z, the enzyme is completely denatured.

6 An aquatic plant is in a test-tube of water. The test-tube is sealed and placed in the dark.

water

Resp>photo
aquatic plant
Yellow

Which changes occur to the concentration of carbon dioxide and oxygen in the water after
two hours?

concentration of concentration of
carbon dioxide oxygen

A decreases decreases
B decreases increases
C increases decreases
D increases increases

© UCLES 2025 0653/23/M/J/25 [Turn over

 4

7 A pregnant woman is told she is deficient in iron.

Which food increases the iron content in her diet?

A cheese
B rice
C milk
D red meat

8 Chemical digestion and physical digestion happen in various parts of the alimentary canal and
associated organs.

In which organ do both processes happen?

A liverIs not an orgn for digestion

B mouthTeeth and salivary amylase
C oesophagus Peristalsis not digestion
D pancreas Is not an orgn for digestion

9 A test-tube is used to model the effect of protease on a protein suspension in the stomach.

test-tube PH is acidic

protease and
protein suspension
at 37 °C

What should be added to the test-tube to provide the optimum conditions for the digestion of
protein?

A amylase
B bacteria
C hydrochloric acid
D lipase

© UCLES 2025 0653/23/M/J/25

 5

10 Water moves through the stomata of leaves during transpiration.

A
Which row shows the direction of water movement and the form of the water?

direction form

A into the leaf liquid

B into the leaf vapour
C out of the leaf vapour
D out of the leaf liquid

11 The diagrams show cross-sections of two different blood vessels.

Vein
Artery
Wider lumen
Narro lumen
P Q

Which type of blood vessel is Q and what is the relative blood pressure in Q compared with P?

relative blood
blood vessel Q
pressure in Q

A artery higher
B artery lower
C vein higher
D vein lower

© UCLES 2025 0653/23/M/J/25 [Turn over

 6

12 Which types of consumer describe the cat in this food web?

1 primary consumer
2 secondary consumer
3 tertiary consumer 3
as
em cat

thrush2 robin3
frog2

sparrow slug caterpillar

plant

A 1, 2 and 3 B 1 only C 2 and 3 only D 3 only

13 What is an ecosystem?

A a network of inter-connected food chains

B a specific area in which a plant or an animal lives
C all the plants and animals that live within a specific area
D the interactions between all living organisms and their environment, in a specific area

14 Which diagram represents molecules of a single compound?

A B C D

15 Which statement about ions is correct?

A Atoms lose or gain electrons to form ions.

B Ions are held together by strong attraction between negative ions.
C Ions form when atoms share electrons.
D Metal ions are attracted to each other.

© UCLES 2025 0653/23/M/J/25