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Microscope Parts and Magnification Guide

The document outlines the parts of a microscope and their functions, including the eyepiece, objective lenses, adjustment knobs, condenser, diaphragm, light source, stage, arm, and base. It also explains how to compute magnification by multiplying the ocular lens power by the objective lens power. Additionally, it provides adjustments needed for different objective lenses to optimize light for viewing specimens.

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medicoexplorer111
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3 views4 pages

Microscope Parts and Magnification Guide

The document outlines the parts of a microscope and their functions, including the eyepiece, objective lenses, adjustment knobs, condenser, diaphragm, light source, stage, arm, and base. It also explains how to compute magnification by multiplying the ocular lens power by the objective lens power. Additionally, it provides adjustments needed for different objective lenses to optimize light for viewing specimens.

Uploaded by

medicoexplorer111
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

MICROSCOPE

PARTS OF THE MICROSCOPE AND THEIR FUNCTIONS

Eyepiece- Contains a magnifying ocular lens that focuses the image from the objective
into your eye.
Objective lens : Has 4X, 10X, 40X, 100X objective lenses of different magnifying power

Coarse Adjustment and Fine Adjustment knobs : They sharpens the image
Condenser-Focuses the light on specimen
Diaphragm (iris or disc)-Regulates amount of light and contrast
Light Source-Illuminates the specimen for viewing.
Stage-Supports specimen in correct location to lens
C-shaped arm : Used for holding microscope
Base: Holds the various parts of the microscope such as light source , adjustment knobs

HOW TO COMPUTE MAGNIFICATION

 Multiply the power of the ocular lens (usually ocular lenses are 10x) times
the power of the particular objective lens you happen to be using at that time.
 The power of each lens is printed on its outer surface: a number such as 10 or 40
followed by an X, meaning the magnification of 10 x 10 = 100, or 40 x 10 = 400.

ADJUSTMENTS OF THE MICROSCOPE

X10 OBJECTIVE:

When the specimens are examined under low power objective, the amount of light
entering the microscopic field must be reduced.

Use concave mirror to direct the light from the light source.

Lower the condenser

If needed, diaphragm may be closed.

X40 OBJECTIVE:

When the specimens are examined under high power, the amount of light entering the
microscopic field must be increased.

Keep the concave mirror position as such.


Slightly raise the condenser.

Open the diaphragm little more.

X100 OBJECTIVE

When the specimens are examined under oil immersion objective power, the amount of
light entering the microscopic field must be very high. (OPR).

Use a plain mirror to direct the light.

Raise the condenser to the maximum.

Open the diaphragm completely.

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