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Heart Dissection Safety and Techniques

The Heart Dissection Guide outlines the objectives, safety protocols, tools, techniques, and procedures for investigating the anatomy of the heart. It emphasizes the importance of respect for biological specimens and safe dissection practices, including the use of protective gear and proper handling of tools. The guide provides detailed instructions for identifying external and internal features of the heart, as well as a cleanup procedure after the dissection.

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0% found this document useful (0 votes)
20 views5 pages

Heart Dissection Safety and Techniques

The Heart Dissection Guide outlines the objectives, safety protocols, tools, techniques, and procedures for investigating the anatomy of the heart. It emphasizes the importance of respect for biological specimens and safe dissection practices, including the use of protective gear and proper handling of tools. The guide provides detailed instructions for identifying external and internal features of the heart, as well as a cleanup procedure after the dissection.

Uploaded by

John appleseed
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Name:___________________________ Lab Partners:____________________

Heart Dissection Guide

OBJECTIVE
● Investigate the anatomy of the heart

● Demonstrate safe work habits when working with biological specimens and dissection tools

● Develop basic dissection vocabulary and techniques

● Demonstrate sensitivity toward, and respect for, living and non-living tissues, specimens, and
organisms utilized for biological research

It is a privilege to work with real specimens. Please treat them with respect. Enjoy the experience and
the exploration of your specimen but DO NOT play with the body parts or animate them.

SAFETY
● Wear protective gloves at all times.

● Inspect your dissection tools; dull, rusted or dirty tools are more unsafe than sharp tools. Ask
your teacher if you have any questions.
● Loose clothing and jewelry must be removed and long hair tied back. Avoid wearing contact
lenses.
● Monitor your partners to ensure that they are having a positive experience. Let your teacher
know immediately if someone is not. Please be encouraging and supportive as the goal is for us
all to have a positive experience with dissections.
● If you feel faint or nauseous at any point sit down and inform the teacher immediately

● Follow all clean up and storage instructions given by your teacher.

TOOLS AND TECHNIQUES


● ALWAYS cut AWAY from you and any of your partners. Be aware of what is along your
cutting line at all times.
● Never use excessive force when cutting. This may be a sign that you are working with dull
tools, you are cutting through too many layers at once or you are using the wrong tool for the
job.
● Scalpels: are used for precision cutting. Hold them like a pencil. Cut with a downward motion,
not pressing too hard. Never use a scalpel for bone or cartilage. Your goal is to preserve the
tissues for examination. Use the scalpel ONLY when absolutely necessary. Use blunt tools as
much as possible. If you have to use a scalpel, try the blunt end first.
● Scissors: are the main tool used in dissections. They are used to cut skin and tissues. Use a
shallow angle, insert into the tissue, lift, and then cut. This will help you to avoid damage to the

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internal structures. Scissors can also be used to separate tissue by inserting them into the area
and then opening them up to separate tissue from one another. This is referred to as the “scissor
technique”. It is unlikely that you will use this technique for this dissection.

PROCEDURE
Collect your dissecting kit and gloves. At any point if you get a rip in your gloves, retrieve a new pair.
As this is fresh material, you must keep your area very clean, and you must wash your hands and your
work area when you are done. Wash your hands before touching anything other than your specimen
and especially before touching your face or eating! All chunks and debris go in the bags provided – not
in the sink or the black garbage bag!

EXTERNAL FEATURES

❑ Locate the tip of the heart or the apex. Only the left ventricle extends all the way to the apex.

❑ Place the heart in the dissecting pan so that the front or ventral side is towards you (the major blood
vessels are on the top and the apex is down). The front of the heart is recognized by a groove that
extends from the right side of the broad end of the heart diagonally to a point above & to your left
of the apex.

❑ While the heart is still in this position in the dissecting pan, locate these blood vessels at the broad
end of the heart:
a. Coronary Artery - this blood vessel lies in the groove on the front of the heart & it
branches over the front & the back side of the heart to supply fresh blood with oxygen
& nutrients to the heart muscle itself.
b. Pulmonary Artery - this blood vessel branches & carries blood to the lungs to receive
oxygen & can be found curving out of the right ventricle (upper chamber to your left)
c. Aorta - major vessel located near the right atria & just behind the pulmonary arteries to
the lungs. Locate the curved part of this vessel known as the aorticarch. Branching from
the aortic arch is a large artery that supplies blood to the upper body.
d. Pulmonary Veins - these vessels return oxygenated blood from the right & left lungs to
the left atrium (upper chamber on your right)
e. Inferior & Superior Vena Cava - these two blood vessels are located on your left of
the heart and connect to the right atrium (upper chamber on your left). Deoxygenated
blood enters the body through these vessels into the right receiving chamber. Use your
probe to feel down into the right atrium. These vessels do not contain valves to control
blood flow.
INTERNAL FEATURES

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❑ Find the pulmonary artery (brings blood from heart to the lungs). Use scissors to cut through the
side of the pulmonary artery and continue cutting down into the wall of the right ventricle. Be
careful to just cut deep enough to go through the wall of the heart chamber.

❑ With your fingers, push open the heart at the cut to examine the internal structure. There might be
some dried blood inside the chambers.

❑ Locate the right atrium. What do you notice about the muscle wall of this receiving chamber?

_____________________________________________________________________________

❑ Find where the inferior and superior vena cava enters the atrium. Do you notice valves?

Yes/No?

❑ Locate the tricuspid valve between the right atrium and right ventricle. Can you identify the three
leaflets? Do you notice the long fibres of connective tissue (chordae tendinae) attaching the valve
to the muscles of the heart? pull firmly (but not too strongly) on one of these fibres. What did you
notice?
___________________________________________________________________________

❑ Use your fingers to feel the thickness of the right ventricle and its smooth lining. Identify the
septum.

❑ Inside the right ventricle, locate the pulmonary artery (which you cut into at the start). Try to find
the pulmonary semilunar valve. Does it feel like the tricuspid?

❑ Using your scissors, continue to cut open the heart. Start a cut on the outside of the left atrium
downward into the left ventricle cutting towards the apex along the centre groove.

❑ Push open the heart with your fingers and examine the left atrium. Find the openings of the
pulmonary veins. From where do these blood vessels come?

________________________________________________________________________

❑ Identify the mitral/bicuspid valve. How many leaflets does it have?___ ________

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❑ Examine the left ventricle. What do you notice about the muscle wall of this heart chamber?

____________________________________________________________

❑ Using your scissors, cut across the left ventricle toward the aorta and continue cutting to expose
the aortic semilunar valve.

❑ Using your scissors, cut through the aorta and examine the inside. How does the aorta feel
compared to other blood vessels?

____________________________________________________________________________

CLOSURE

❑ Clean up. Dispose of any materials in the correct location. Clean and dry your lab equipment as
directed. Have your teacher check your lab space after you clean up.

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Common questions

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The pulmonary circuit involves the right side of the heart and includes the right atrium, right ventricle, and pulmonary arteries, directing blood to the lungs for oxygenation. Observations during dissection show thinner walls in these chambers, as less force is needed. Contrastingly, the systemic circuit involves the left side, including the left atrium, left ventricle, and aorta, which pumps oxygenated blood to the body, requiring thicker muscular walls in the left ventricle for higher pressure. These structural differences reflect their functional roles in circulation .

The groove on the heart's ventral side extends from the right side of the broad end of the heart diagonally to a point above and to the left of the apex. This groove is crucial in identifying the anatomical position of the heart as it indicates the front or ventral side of the heart, which should be aligned toward the researcher during dissection. This orientation ensures accurate identification of major blood vessels and internal features such as the coronary artery and ventricles .

The primary function of the coronary artery is to supply fresh blood rich in oxygen and nutrients to the heart muscle itself. This is critical because the heart, being a muscle, requires a constant supply of oxygen and nutrients to function properly. Without adequate blood supply from the coronary artery, the heart tissue could become ischemic, leading to complications such as heart attacks. The coronary artery branches over the front and the back side of the heart as outlined in the dissection guide to ensure a comprehensive coverage of the heart muscle .

The structural differences observed between the right and left ventricles include the thickness of the heart muscle wall. The left ventricle has a much thicker wall compared to the right ventricle. This difference is significant because the left ventricle is responsible for pumping oxygenated blood throughout the entire body, requiring higher pressure and more force, whereas the right ventricle only pumps deoxygenated blood to the lungs. The thicker wall of the left ventricle reflects the need for greater contractile force .

The evidence indicating the absence of valves in the inferior and superior vena cava comes from the inability to find any valves during dissection. It is noted that these vessels connect to the right atrium and allow the passage of deoxygenated blood into the heart, but no valves were observed to regulate this flow, which suggests that the flow of blood into the heart chambers through the vena cava is continuous and unregulated by valves .

To ensure a safe and respectful dissection environment, protective gloves must be worn at all times, and all dissection tools should be inspected for any dullness, rust, or dirt. Loose clothing and jewelry should be removed, and long hair tied back. Contact lenses should be avoided. The work area must be kept clean, and hands washed before touching anything else especially the face or food. Additionally, respectful treatment of specimens with no play or animation of the parts is important, as well as encouraging a positive experience for all participants and notifying the teacher of any issues .

Heart valves are designed with structures like leaflets and chordae tendineae that facilitate unidirectional blood flow and prevent backflow. During dissection, the tricuspid and mitral valves can be observed with their leaflets and connective tissue fibers, confirming their role in opening to allow blood flow and closing tightly to prevent backflow. The observation that pulling on the chordae tendineae demonstrates their connection to heart muscles helps highlight how this design maintains valve function during contraction and relaxation of heart muscle .

The chordae tendineae, observed during heart dissection, are long fibers of connective tissue that attach the valve leaflets to the heart muscles. Their role is to anchor the valves in position and prevent them from inverting during ventricular contraction, thereby ensuring valves function properly to maintain unidirectional blood flow. The observation of their firm attachment and function upon being pulled highlights their critical role in supporting valve closure during systolic pressure changes .

Proper dissection techniques, such as cutting away from oneself and using minimal force, prevent damage to internal heart structures. The use of a scalpel for precision cutting with a downward motion and scissors for skin and tissue cutting at a shallow angle ensures that the tissues are preserved for examination. Additionally, using the 'scissor technique' helps in separating tissues without damaging the internal structures. By following these guidelines, the risk of inadvertently damaging the heart's internal framework is minimized .

Using specific tools like scissors and scalpels is essential for maintaining precision and ensuring the integrity of the specimen. Scalpels allow for precision cutting, ideal for delicate tasks that require minimal force, while scissors are used mainly for cutting skin and tissue, as they can separate tissues without excessive pressure or damaging the structures beneath. This tool specificity helps prevent accidental damage during dissection, preserving the anatomical features for study .

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