Instruments
Bone marrow needle
1. Identify the
instrument
2. List two identifying
features
3. List two indications
where this is used
4. Mention any 2 sites
where this
instrument is used.
1. Bone marrow (aspiration)needle
2. It has guard, side screw and stylet
3. 4 indications - Anemia, Leukemia, Lymphoma and
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Multiple myeloma
4. Sites- Posterior iliac crest and sternum
Lumbar puncture needle
1. Identify the instrument
2. List two identifying features
3. List two indications where
this is used
[Link] puncture needle
[Link] Points – large spinal needle, 22gauge
[Link] : Therapeutic and diagnostic
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Therapeutic - Spinal anesthesia, Intracranial antibiotics
Diagnostic - Meningitis and CNS lymphoma
Neubauer chamber
1. Identify the instrument
2. List two identifying
features
3. List two indications where
this is used?
1. Neubauer chamber
2. Identification Point - The frame of the counting
chamber consists of 9 large squares each with a 1 mm2
area.
3. Uses: Counting chamber used for counting RBCs,
WBCs, Absolute eosinophil count, platelets, sperms and
cells in body fluids.
ESR tube
1. Identify the instrument
2. List two identifying features
3. List two causes of increased value.
1. Westergren Tube
2. Identification Points – 30cm long, 2.5 mm diameter.
Glass pipette open at both ends. Markings from 0 to 200.
3. Increased ESR - Tuberculosis, Anemia, Multiple
myeloma and Rheumatoid arthritis.
PCV tube
1. Identify the instrument
2. List two identifying
features
3. List two indications where
this is used
1. Wintrobe tube
2. Wintrobe tube has a length of 110mm and internal
bore of 2.5mm. Graduated from 0 – 10 in both
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directions. Tube is closed at one end.
3. Determination of ESR, PCV.
Preparation of buffy coat smear.
RBC pipette
1. Identify the instrument
2. Name the diluting fluid
used for this cell counting.
3. List two indications where
this is used
1. RBC pipette
2. Hayem’s fluid, Dacie’s fluid
3. Used wherever high dilution is required. Eg: high
leukocyte count and total sperm count.
WBC pipette
1. Identify the instrument
2. Name the diluting fluid used
for this cell counting.
3. List two indications where
this is used
1. WBC pipette
2. Turk’s fluid
3. Sperm count, cell count in CSF, High total leukocyte
count
Sahli’s haemoglobin Pipette
1. Identify the instrument
2. List two identifying
features
3. List indications where this
is used
1. Sahli’s haemoglobin Pipette
2. Marked at 20µl or 0.02ml. No bulb.
3. To estimate haemoglobin using acid hematin method.