Stratified Sampling
Scenario
A clinical laboratory processed 500 blood samples in one week:
- 250 from male patients
- 200 from female patients
- 50 from children
A sample of 50 is required for quality assurance.
Step 1: Define Population and Strata
The total population is N = 500. The population is divided into three strata based on
gender/age:
• Stratum 1: Male (N₁ = 250)
• Stratum 2: Female (N₂ = 200)
• Stratum 3: Children (N₃ = 50)
Stratification ensures that each subgroup is properly represented in the final sample.
Step 2: Decide Total Sample Size
The total sample size to be selected is n = 50.
Step 3: Proportional Allocation
For each stratum, the number of samples to select (nₕ) is determined using the formula:
nₕ = (Nₕ / N) × n
Where:
• nₕ = number of samples from stratum h
• Nₕ = population size of stratum h
• N = total population size
• n = total sample size
Or
For Males:
n₁ = (250 / 500) × 50 = 0.5 × 50 = 25
For Females:
n₂ = (200 / 500) × 50 = 0.4 × 50 = 20
For Children:
n₃ = (50 / 500) × 50 = 0.1 × 50 = 5
Step 4: Random Selection Within Each Stratum
After determining the number of samples for each stratum, simple random sampling is
applied within each group to select the required number of samples. Methods such as
random number tables, lottery method, or Excel functions can be used. Each sample within
a stratum has an equal chance of being selected.
Step 5: Combine the Selected Samples
The selected samples from each stratum are combined to form the final sample of 50. This
ensures proportional representation from each subgroup.
Step 6: Record and Report
The following information should be recorded clearly:
• Total population and strata details
• Sample size allocation formula and results
• Random selection method used
• Final list of selected samples