Unit 05 Study Guide - DNA Replication and Protein Synthesis
1. Describe the experiment or contribution of each of the scientists below.
Frederick Griffith:
Oswald Avery:
Alfred Hershey and Martha Chase:
Erwin Chargaff:
James Watson and Francis Crick:
Rosalind Franklin and Maurice Wilkins:
For questions 2 and 3, refer to the figure below.
2. Identify the structure above.
3. Label each component in the structure above.
4. Describe the overall shape of DNA.
5. Describe what is meant by the term antiparallel.
6. Identify the bond between the sugar and phosphate in the DNA backbone.
7. A sample of DNA contains 24% adenine. Determine the percentages of the other three nitrogen bases.
8. The location where DNA replication begins on the double helix is called the replication ___ or ____.
9. Describe the functions of the key enzymes used in replication.
helicase: DNA polymerase:
primase: ligase:
10.Describe the difference between the leading strand and the lagging strand.
11.Explain the function of primers.
12.On which strand do you find Okazaki fragments?
13.Compare and contrast DNA and RNA using the table below.
DNA Both RNA
14.Construct a diagram to illustrate the Central Dogma of Biology.
15.Define/describe the following terms:
gene -
tRNA -
mRNA -
anticodon -
codon -
rRNA -
16.Indicate where in the cell each process occurs.
DNA Replication Transcription Translation
17.Use a codon chart to determine which mRNA codons and amino acids would be formed by the following DNA
sequence.
DNA Sequence T A C G T T A C G T A G A C T
mRNA Sequence
Amino Acid Sequence
18.Describe the following terms.
RNA polymerase –
19.Describe the steps of Transcription.
Initiation
Elongation
Termination
20.Describe the steps of Translation.
Initiation
Elongation
Termination
21.Label the diagram below.
22.Describe the process of mRNA processing. In your answer, include the terms pre-mRNA, mRNA, exons, introns,
splice/spliced/splicing.
23.Identify the type of gene mutation shown in the diagram to
the right.
24.Identify the three types of gene mutations discussed in class.
25.Describe what is meant by the term frameshift.
26.What kind of mutations can cause a frameshift?
27.Describe what is meant by each term:
missense mutation –
nonsense mutation –
silent mutation –