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Cambridge O Level Biology: Cell Movement

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0% found this document useful (0 votes)
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Cambridge O Level Biology: Cell Movement

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milkyhilly451
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© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd

Cambridge O Level Biology (5090) – Unit 3:

Movement into and out of Cells

Q1. Diffusion (5 marks)


(a) Define diffusion. (2)
(b) State two factors that affect the rate of diffusion. (2)
(c) Give one example of diffusion in living organisms. (1)

Q2. Osmosis basics (6 marks)


(a) Define osmosis. (3)
(b) Explain why osmosis is a type of diffusion. (2)
(c) State one feature of a partially permeable membrane. (1)

Q3. Osmosis in plants (6 marks)


(a) Explain what happens to a plant cell placed in pure water. (3)
(b) Explain what happens to a plant cell placed in a concentrated salt solution. (3)

Q4. Osmosis in animals (5 marks)


(a) Describe the effect of placing red blood cells in pure water. (2)
(b) Describe the effect of placing red blood cells in concentrated salt solution. (2)
(c) Suggest why these effects are different from plant cells. (1)

Q5. Experimental work (5 marks)


Describe how you would use potato cylinders to investigate the effect of sucrose concentration on
osmosis. Include variables you would measure and control.

Q6. Active transport (6 marks)


(a) Define active transport. (2)
(b) Explain why active transport requires energy. (2)
(c) Give one example of active transport in plants and one in animals. (2)

Q7. Comparison (5 marks)


Complete a table to compare diffusion, osmosis and active transport under the following headings:
- Requires energy
- Requires a membrane
- Substances moved
- Direction of movement (up or down concentration gradient)

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In pure water, plant cells become turgid due to the influx of water into the cell vacuole, leading to firm cells, which is counteracted by the rigid cell wall providing structural support . In contrast, red blood cells in animals may burst in pure water due to a lack of structural reinforcement; the influx of water causes the cells to swell and possibly lyse since animal cells lack rigid cell walls .

In plants, active transport is exemplified by the uptake of mineral ions from the soil into root cells, crucial for nutrient acquisition even when soil concentrations are lower than inside cells . In animals, the sodium-potassium pump actively moves Na+ out of and K+ into cells, vital for nerve impulse transmission and cellular homeostasis .

Osmosis illustrates diffusion as it involves the movement of water molecules from a region of higher water potential to a region of lower water potential, effectively moving down a concentration gradient. What makes it unique is the necessity of a partially permeable membrane which selectively allows only water molecules to pass through, differentiating it from general diffusion which may not require a membrane .

Active transport requires energy because substances move against their concentration gradient, from areas of low concentration to high concentration, which is thermodynamically unfavorable. Energy, typically from ATP, is needed to fuel transport proteins to overcome this barrier . In contrast, diffusion and osmosis involve movement down the concentration gradient, occurring spontaneously without the need for energy input .

The experiment involves submerging potato cylinders in varying concentrations of sucrose solutions and measuring the changes in their mass or length over time, indicating osmotic activity. Key variables to control include temperature, potato cylinder size, initial mass, time in solution, and ensuring all containers are sealed to minimize evaporation and external contamination .

Partially permeable membranes permit the selective passage of water molecules while restricting solute movement. This selectivity is crucial for maintaining cellular homeostasis by controlling osmotic balance in cells and tissues . In experiments, using such membranes helps isolate factors affecting osmosis, such as solute concentrations, enabling precise study of water movement dynamics .

Diffusion does not require energy, does not necessarily require a membrane, can move gases or small molecules, and occurs down the concentration gradient . Osmosis, as a specific type of diffusion, also does not require energy, requires a partially permeable membrane, involves the movement of water, and occurs down the water potential gradient . Active transport, in contrast, requires energy (usually from ATP), often requires a membrane, moves ions or molecules like nutrients, and occurs against the concentration gradient .

To prevent hemolysis, intravenous solutions should be isotonic relative to blood plasma. This ensures no net water movement into or out of red blood cells, maintaining cellular integrity. Careful selection of salt or glucose concentrations in IV fluids is crucial, and regular monitoring of a patient's fluid balance can prevent complications associated with osmotic imbalances .

Factors affecting diffusion rate include temperature, which increases kinetic energy and molecular movement, and concentration gradient steepness, where a larger difference enhances diffusion rate. Surface area impacts exposure to external environments, while the diffusion medium and distance affect movement resistance and time, respectively . These factors are critical in biological systems for efficient resource transport, waste removal, and ensuring proper metabolic function .

In concentrated salt solutions, plant cells lose water due to osmotic movement, leading to plasmolysis where the cell membrane detaches from the cell wall. This results in decreased turgor pressure, loss of structural integrity, and impaired cellular processes. Prolonged exposure may lead to wilting, growth inhibition, and potential plant death .

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