Sun and shade leaf X10
epidermis
Palisade – 2 layers
Spongy mesophyll
Lower epidermis
SHADE LEAF
All tissues generally less
developed / thinner
Hakea leaf or stem?
Round in x-
section
=needle shape
reduce surface
area
Thick cuticle
Epidermal hair
Sunken stoma
Sunken stoma
Palisade (3 layers)
Stained red/brown
Sub-stomatal air
chamber
pericycle
phloem
xylem
Patches of
sclerenchyma
Laticiferous cells
Parenchyma cells
Stoma w.m. X100
Whole mount
Thick inner wall
Chloroplast in
guard cells
Granular nucleus
Stoma opening
Stoma w.m. X100
Granular nucleus
Epidermal cells
(no chloroplast)
Pinus Leaf t.s. X40
epidermis
Resin canal
Vascular tissue in
stele
Mesophyll cells
Pinus Leaf t.s. X400
Epidermal cells
with thick wall
collenchyma
Photosynthetic
cells have
indented walls
Resin canal
Pinus Leaf t.s. X400
parenchyma
Vascular bundle
Endodermis –
thickened outer wall
Pinus Leaf t.s. X400 ~ vascular tissue
Xylem tracheid –
(no vessels)
characteristic of
gymnosperms
phloem
cambium
Layer of thick
wall cells
Pinus Leaf t.s. X40 ~ stoma
Sunken stoma
Guard cell
Indented wall
Pigmented
cytoplasm with
choroplast
Pinus Leaf t.s. X40 ~ distribution stoma
Positions
where
stoma are
located