Bacterial Genome Sequencing Module Results
Translated by
ScribdTranslationsBacterial Genome Sequencing Module Results
Translated by
ScribdTranslationsStarted
Friday, November 10, 2023, 5:26 PM
the
FinalState
Finished
Friday, November 10, 2023, 5:28 PM
in
Time
1 minute 50 seconds
employee
Points 12.00/12.00
Rating 100.00 out of 100.00
Congratulations! You have passed the Module.
Comment
o- Please return to the main course page to
continue with the next module.
Question 1
Correct
It scores 1.00 out of 1.00
Mark question
Statement of the question
The genome of a bacterium is:
a.
Exclusively the DNA that forms its chromosome
b.
The total set of DNA that one possesses
c.
Exclusively the DNA that is part of its plasmids
Feedback
The correct answer is: The total set of DNA that one possesses
Question2
Correct
It is scored 1.00 out of 1.00
Mark question
Question statement
The genes that confer resistance:
a.
They are always found in the bacterial chromosome.
b.
They are always found in bacterial plasmids.
c.
They can be present both in the bacterial chromosome and in plasmids.
Feedback
The correct answer is: They can be present both in the bacterial chromosome and in plasmids.
Question 3
Correct
It scores 1.00 out of 1.00
Mark question
Statement of the question
Unlike Sanger sequencing, __________ sequencing or __________ sequencing
generation does allow sequencing the complete genome of an organism.
a.
Massive / first
b.
Massive / second
c.
Intensive / second
Feedback
Massive / second
Question4
Correct
It scores 1.00 out of 1.00
Mark question
Question statement
The most widely used chemistry for sequencing today is the so-called
by synthesis, and is based on the generation of complementary chains to the molecules
DNA originals and the emission of fluorophores, which are detected by a system:
a.
Optical
b.
Chemical
c.
Mechanic
Feedback
The correct answer is: Optical
Question5
Correct
It scores 1.00 out of 1.00
Mark question
Statement of the question
In the massive sequencing of bacteria:
a.
A single DNA molecule is sequenced.
b.
It is necessary to fragment the genome and then reconstruct it using tools.
informatics
c.
The step of DNA extraction is omitted.
Feedback
The correct answer is: It is necessary to fragment the genome and then reconstruct it.
of computer tools
Question6
Correct
It is scored 1.00 out of 1.00
Mark question
Statement of the question
It is the workflow for massive sequencing:
a.
Generation of genomic libraries / sequencing / DNA extraction
b.
Sequencing / DNA extraction / generation of genomic libraries
c.
DNA extraction / genomic library generation / sequencing
Feedback
The correct answer is: DNA extraction / genomic library generation / sequencing
Question 7
Correct
It scores 1.00 out of 1.00
Mark question
Statement of the question
A genomic library is a random collection of DNA fragments that have been
coupled adapter sequences and __________ (these will allow to identify the origin of the
fragment with the sample of origin.
a.
Indices
b.
Indicators
c.
Incrementers
Feedback
The correct answer is: Indices
Question8
Correct
It scores 1.00 out of 1.00
Mark question
Statement of the question
For each sample that we want to sequence, a genomic library is generated; however,
at one point in the process all the libraries come together and form a _________. This must
to contain all samples properly represented, so the step of _________ is
crucial.
a.
Pool / sequencing
b.
Fragment / normalization
c.
Pool / normalization
Feedback
The correct answer is: Pool / normalization
Question 9
Correct
It is scored 1.00 out of 1.00
Mark question
Statement of the question
With the data generated by the sequencer, it is possible to obtain relevant biological information:
a.
Without taking any additional steps
b.
Once the required bioinformatics analyses are completed
c.
Performing the analyses on the sequencer itself
Feedback
The correct answer is: Once the required bioinformatics analyses are carried out
Question 10
Correct
It is scored 1.00 out of 1.00
Mark question
Statement of the question
The importance of second-generation sequencing for Resistance to
Antimicrobials are based on:
a.
It is very costly and only allows for the identification of RAM genes.
b.
It allows for the characterization of pathogenic agents, identifying the RAM genes they possess, their genetic context.
and variants. Additionally, it facilitates the making of relevant comparisons in research of
sprouts
c.
It offers results comparable to those obtained through traditional techniques.
Feedback
The correct answer is: It allows characterizing the pathogenic agents, identifying the RAM genes that
it has its genetic context and variants. Additionally, it facilitates the making of comparisons
relevant in outbreak research
Question 11
Correct
It is scored 1.00 out of 1.00
Mark question
Statement of the question
The identification of RAM genes through WGS (whole genome sequencing, for its
acronyms in English:
a.
Ensure that the insulation will show resistance, verifiable through susceptibility testing.
antimicrobials (AST)
b.
It is not an indicator of the level of resistance that the bacteria may have, for which it is necessary to carry out
antimicrobial susceptibility tests
c.
It will completely replace antimicrobial susceptibility testing very soon.
Feedback
The correct answer is: It is not indicative of the level of resistance that the bacteria may have, for which
susceptibility tests for antimicrobials are required
Question12
Correct
It is scored 1.00 out of 1.00
Mark question
Statement of the question
Some of the limitations associated with mass sequencing are:
a.
Lack of personnel trained in molecular biology techniques
b.
Inability to obtain relevant biological information even with trained personnel in
bioinformatics and sufficient computing capacity
c.
Lack of personnel trained in bioinformatics and limited computing capacity for both
processing, such as storage
Feedback
The correct answer is: Lack of staff trained in bioinformatics and limited capacity of
computing for both processing and storage