Creatinine Purification Analysis Report
Translated by
ScribdTranslationsCreatinine Purification Analysis Report
Translated by
ScribdTranslationsBiochemistry Subject
(PRACTICE) No. 11
IV Cycle of Studies Academic Semester 2021-I
Determination of the purification of
creatinine
Theme
Authors
• Blas Dávila Mauricio 74314473
• Borrero Ulfe Michelle Daniella 71573942
• Cubas Saavedra Lucero 72696945
• Díaz Meza Marco Antonio 72973194
• León Fallaque Valeria Patricia 72112763
• Rufino Daira Katherine Mesones 77040873
• Monteza Zagaceta Jhon Albert 77488175
• Mayra Elizabeth Razuri Terán 72650391
• Rosario Córdova Ernesto Moisés 72085028
• Sirlopu Bocanegra Julián Ricardo 72931064
• Tamayo Huamán Fernando Anghelo 72791899
• Tejada Farfán Greece Mercedes 73826071
• Ugaz Soto Carlos Tomás 77675776
• Vásquez Espinoza Nicolle Alejandra 71553086
Group: 11
Teacher
Dr. Gaudhy Chávez Pasco
Chiclayo - Peru
2021
INTRODUCTION
We will begin this report by providing a brief definition of the topic to be addressed.
Creatinine is a waste molecule that is generated from metabolism.
muscular. It comes from creatine, which is very important for energy production.
muscular. Approximately 2% of the body's creatine is converted into
creatinine every day. Creatinine is transported from the muscles through the
blood towards the kidney. The kidneys filter most of the creatinine and eliminate it in
urine. Although it is a waste substance, creatinine is a test
essential diagnosis, as it has been observed that its concentration in blood indicates
with a good reliability the status of renal function. If the kidneys do not function
Well, they do not eliminate creatinine properly and therefore it accumulates in the blood.
this creatinine can indicate a possible dysfunction or renal insufficiency,
even before symptoms appear. That's why creatinine is often listed in
the blood tests that are commonly performed. The determination of creatinine
it is a useful indicator for evaluating renal glomerular function, taking into account the
Prerequisite that the production of creatinine and its excretion are equal. This is
it occurs in healthy individuals with a normal diet. We can say that the elimination of
creatinine over a 24-hour period is a constant value, dependent
mainly of the individual's muscle mass, and on the other hand the calculation of
creatinine clearance will be a direct parameter of renal function.
OBJECTIVES
1. Determine the concentration of creatinine in plasma and urine using a
spectrophotometric method
.
2. Determine the creatinine clearance with the values in plasma and urine.
3. Compare the obtained value of creatinine clearance with the ranges of
reference.
QUESTIONNAIRE
1. Create a diagram about the metabolism of creatinine.
(1).
2. What other test is being replaced by creatinine clearance?
First of all, we must take into account that the debugging of insulin is
that commonly accepted method to evaluate the filtration rate
glomerular (GFR), however, due to its difficulty, it is not carried out
frequently in clinical practice. Consequently, the need has arisen
to seek other markets that provide greater ease both as
precision for determining TGF.
Where those serum creatinine levels are not taken into account as
a unique average for estimating renal function, particularly for the detection of
early periods of chronic kidney failure as in cases of disease
advanced renal.
Therefore, certain exogenous markers have been proposed as options, such
such as radioactive contrast agents, such as in the case of
Ethylenediaminetetraacetic acid (EDTA), Iothalamate, and Technetium-99m-acid
diethylenetriaminepentaaceticTc99-DTPA).
On the other hand, different formulas have also been developed to assess the
renal clearance, with one of the most used being the Cockcroft proposal
Gault, and lately the one developed by Levey as part of the MDRD study.
(2).
What substances can interfere with the determination of creatinine?
In creatinine tests where the sample is serum, plasma, or urine,
it will produce an interference with substances in the following cases (3):
Ascorbic acid greater than 1.00 mg/dL.
Hemoglobin greater than 400 mg/dl (4 g/l).
Bilirubin greater than 28 mg/dl.
4. Why is creatinine preferred in the study of function?
glomerular?
Reliable estimation of kidney function is a necessity in the
clinical practice, which has driven efforts throughout history to
find better biomarkers and estimation formulas for speed
glomerular filtration rate (GFR). Creatinine has established itself in history as
the preferred biomarker to estimate GFR and its measurement has gone
perfecting over time, leading to progressively more quantifications
exact methods. The use of enzymatic measurement methods for creatinine allows
obtain a more real value than with Jaffe methods, especially in individuals
with normal renal function. The change in measurement from colorimetric methods
by Jaffe to enzymatic assays has been fundamentally limited by
economic reasons (4).
5. How would you interpret a result of 1.3 mg/dL in a young woman?
young and muscular man and an elderly person?
Compared to the reference values, it could be stated that: Values
references:
All three patients have a slightly elevated level of creatinine in their blood.
Creatinine levels can temporarily increase if you are dehydrated.
(It could be due to excessive exercise or lack of fluid intake), having a
low blood volume, eat a lot of meat or take certain
medications. If the serum creatinine level is above normal, it is possible
that the doctor recommends confirming the results with another urine test or
blood test. If kidney damage is a concern, it is important to monitor
any disorder that may contribute to the damage. It is especially important
control blood pressure, which often requires taking medication. Not
Permanent kidney damage can be reversed, but with proper treatment.
you can prevent further damage (5).
PROCEDURAL PART
1. Are the creatinine clearance values present in both cases?
normal or altered?
The cases presented for this question are:
Case 1:
• Male patient, 45 years old
• 82 mg/dL
• 1.07 mg/dL
• 24-hour urine volume: 1800 ml
Case 2:
• 70-year-old female patient
• 50 mg/dL
• Plasma creatinine: 1.07 mg/dL
• 24-hour urine volume: 1500 ml
And the values that we estimate as normal are the following:
Taking that into account, we can now determine if the creatinine clearance
is within the normal ranges using the following formula:
By substituting the values, we can obtain:
Case 1:
Case 2:
With the results already obtained, we can say that the creatinine clearance
is normal in Case 1 (95.79 ml/min) and altered in Case 2 (48.67)
ml/min).
2. Why despite having equal plasma creatinine values the
Is creatinine clearance different in both cases?
It is different in both cases, due to the creatine values in urine as well.
since in volume they are totally different, in which the given formula will result in
as a result a completely different number.
3. How do you interpret these laboratory results for both cases?
CASE 1 CASE 2
82 mg/dL 50 mg/dL
1.07 mg/dL 1.07
24-hour urine volume: mg/dL
1800 ml 24-hour urine volume:
1500 ml
INTERPRETATION
As the patient is male and 45. As the patient is female,
years, their plasma creatinine values their plasma creatinine is within the
are within the normal range. normal range.
In the case of creatinine in urine, it
consider that it is below the range of
reference what could mean some reference that is from 72-110.
muscular or neuromuscular disorder.
4. According to the tests performed, the patient in case 2 will require
of renal dialysis?
No, because their creatinine levels are within the normal ranges.
except for urine creatinine, but that value is not sufficient reason to
to perform dialysis on him, not without first conducting other clinical analyses.
5. If the urine volume were 900 ml in case 1, how would it alter the
creatinine clearance values?
If the urine volume were 900 ml, the patient would have a result of 47.8 ml/min.
that is to say, that their results would be very low, which would not be within
the normal.
CONCLUSIONS
The electrophoretic method will be used to determine the concentration of
creatinine both in a blood sample and in urine, from the
Jaffe reaction, which is based on a reaction of the sample with the
picric acid, resulting in an orange-colored complex.
2. After conducting the creatinine clearance calculation, it resulted in
a low value for the second case, which may indicate problems
renal such as damage to tubular cells, renal failure and
heart failure.
3. Well, in all the results when compared to the reference table
in the observed cases, it was noted that in case 1 everything was inside
the normal one, in which for case number 2 it was not the same since the patient
the clearance level was too low from normal, 48.6 of clearance
of creatinine.
BIBLIOGRAPHIC REFERENCES
1. Creatinine Metabolism and Urea Synthesis [Internet]. Studocu. 2021
[quoted 3 June 2021. Available
Unable to access content from the provided URL.
colombia/renal-system/summaries/metabolism-of-creatinine-and-synthesis-
de-urea/5265210/view
2. Hernández J, Torres A, Rodríguez F. Comparison of four methods of
measurement of glomerular filtration rate with inulin clearance
healthy individuals and in patients with kidney failure. Nephrology
(Madrid) [Internet]. 2010 [cited 2021 May 29]; 30(3): 324-330. Available
The provided text does not contain translatable content.
69952010000300009&lng=es.
[Link] Lab. Creatinine: Enzymatic method for the determination of
serum creatinine, plasma or urine (Internet). Argentina; 2000 (consulted)
May 23, 2021). Available at: Invalid URL provided. No text to translate.
[Link]/VademecumDocuments/Vademecum%20english/creatinine_e
nzimatica_aa_liquida_sp.pdf
4. Huidobro J, Tagle R, Guzmán A. Creatinine and its use for estimation of the
glomerular filtration rate. Medical Journal of Chile. 2018;
146(3):344-50.