1)
2) amount : 8-10 ml
~ each set : 1 aerobic & 1 anaerobic bottles
Conventional culture media:
a) monophasic : BHI broth
b) biphasic : castaneda
3) It help to: increase the yield of diagnosis
~ determine intermittent bacteremia
~ Differentiate b/w commensals &pathogen
4) ~ keep at 35*C in an incubator (if
available )or keep at room temperature.
~ never be refrigerated .
1) CSF
2) CSF is obtained by lumbar puncture under
strict aseptic conditions.
~ Hand wash
~ wear hand glove
~disinfect the site( b/w L3 & L4) with 70%
alcohol & allow to dry.
~ disinfect with Povidone iodine in concentric
inside out & allow to dry
~ collected CSF is divided into 3 sterile
containers:
Cell count, biochemical analysis, bacteriological
examination
3) keep in an incubator at 37*C
1) ~ surgical debridment
~ wash with sterile saline
~ sterile swab - from depth of wound without
touching the super cial area
~ immediately transport to lab without any
delay.
2) name
Age
Sex
IP no
Unit
Ward
Clinical symptoms
Provisional diagnosis
Sample & type
Treating physician
1) sputum
2)2 samples are collected- spot & early
morning.
~ early morning sample should be collected in
empty stomach, after rinsing the mouth well.
~ ask patient to inhale deeply 2-3 times & cough
out the sputum from chest during exhalation.
~ spit the sputum into wide mouthed screw
capped container.
~ quantity : 3-5 ml
~ quality : thick & purulent (salivary / watery
sample rejected)
3) refrigerated
1) catheterised patient: urine is collected from
proximal part of catheter tube after clamping
distally,
~ disinfecting the proximal part with 70%
alcohol
~ aspirate with a sterile needle & syringe.
~ do not collect from urobag.
2)if delay expected >1-2 hrs,
~ refrigerated
~ adding boric acid (upto 24 hrs)
1)
2)adult : 8-10 ml , 1:5 dilution
Children : 1-3 ml , 1:10 dilution
3) it help in the dilution of antimicrobial
substances present in the collected blood.
4) keep at 35*C in an incubator (if available )
or keep at room temperature.
~ it never be refrigerated
1)ask the patient to properly clean the urethral
meatus or glans
~ initial urine is discarded.
~ Clean voided midstream urine is collected
~ collection bottle: wide mouthed screw capped
sterile container.
~ only 3/4 th of bottle is to be lled.
2) on bottle: name
Age
IP no
Sample
Request form:
name
Age
Sex
IP no
Unit
Ward
Clinical symptoms
Provisional diagnosis
Sample & type
Treating physician
3)if delay expected >1-2 hrs,
~ refrigerated
~ adding boric acid (upto 24 hrs)
1)ask the patient to properly clean the urethral
meatus with water.
~ initial urine is discarded.
~ Clean voided midstream urine is collected
~ collection bottle: wide mouthed screw
capped sterile container.
~ only 3/4 th of bottle is to be lled.
2) same as above qstn.
3) same as above qstn.