Water Quality Analysis Laboratory Practice
Water Quality Analysis Laboratory Practice
SEMESTER : X
JULIACA - PERU
2023
Table of contents
THEME : Laboratory practice: Water analysisa ................................. 1
I. INTRODUCTION. 4
II. OBJECTIVES. 4
Quality of water. 7
4.4. Standards international standards for water quality for human consumption
established by the World Health Organization. 8
Turbidity. 12
V. METHODOLOGY. 12
Samples. 12
Materials ...................................................................................................................... 13
Clothing. 13
5.2. Procedure. 13
VI. DEVELOPMENT OF THE WORK. 14
VII. CONCLUSIONS. 16
VIII. RECOMMENDATIONS. 17
X. ANNEXES. 20
XI. QUESTIONNAIRE. 22
I. INTRODUCTION
II. OBJECTIVES
2.1. General objective
Evaluate and understand comprehensively the effectiveness of the multiparameter in the analysis
of water, through the execution of precise measurements of temperature, pH, oxygen
dissolved and electrical conductivity.
The current situation in river water sampling has revealed a worrying picture.
in the river in question. Water quality is compromised, showing levels
significant pollution. This will be reflected when analyzing the water in the
multiparameter. The presence of floating household waste and waste in the
riverbanks suggest a lack of proper waste management, contributing to
deterioration of the aquatic environment. In addition, the activity of washing clothes in the
proximities of the river add polluting elements, such as detergents, that can
affect the chemical composition of the water. The presence of fish in this environment
raises concerns about the health of aquatic fauna, which is exposed to
suboptimal conditions. Together, this description underscores the urgency of addressing
the problems of pollution and promote sustainable practices to preserve the
water quality in this river ecosystem.
analysis covers key factors such as the concentration of heavy metals, levels of
nitrates, pH, and the presence of indicator microorganisms of fecal contamination.
The following are the policy guidelines at the national, regional, and local levels.
and relevant sectors to carry out field activities (Organization
Pan American Health Organization, 2012:
[Link]
It is a renewable natural resource, largely deteriorated by pollution.
anthropogenic and environmental degradation is affected by inadequate use and
conservation provided by the population. This resource can be scarce in one part of the
year and in certain geographic areas; but it can also be excessive and destructive in
others (J. Sánchez, 2019).
[Link] quality
The characteristics that determine water quality refer to its aspects
physical, chemical, and microbiological. According to these characteristics, the quality
a body of water can be suitable for a specific use and an inadequate use. The
World Health Organization (2006) defines water quality: 'set of
physical-chemical or microbiological variable characteristics, as well as their values
of acceptance or rejection. The physicochemical quality of the water is based on the
determination of specific chemical substances that can affect health after
short or long periods of exposure. The evaluation of water quality is a
multi-faceted approach regarding natural quality" (Ministry of
Environment, 2013).
[Link] standards for the quality of water for human consumption
established by the World Health Organization.
Since 1958, the World Health Organization (WHO) has issued on a
regulate the 'International Drinking Water Standards', which were subsequently
designated as the 'Guidelines for Drinking Water Quality'. These guidelines
International organizations aim primarily to raise quality standards of
drinking water and safeguarding human health, serving as a fundamental reference
for the formulation of regulations on drinking water quality in nations of
everyone and establish the appropriate standards for drinking water, WHO
you must conduct an investigation and subsequent analysis that allows you to verify whether those
standards fulfill their main mission of protecting public health. (Truque, 2003)
All countries that adopt national regulations of this type use the
WHO Guidelines for Drinking Water Quality as the main reference
comparative. These guides, published approximately every 12 years, compile the
latest global information on the topic, serving as the framework of
updated reference for the evaluation and regulation of water quality
potable (Pan American Health Organization, 2012).
Eutrophication
Turbidity
Turbidity is caused by suspended particles or colloids (clays, silt,
finely divided earth, etc.). The turbidity is caused by the particles that
they form colloidal systems; that is to say, those that by their size, are found
suspended particles reduce the transparency of water to a greater or lesser extent. The
V. METHODOLOGY
Materials and Equipment
Samples
River water collected on November 29
River water freshly collected on the same day from the laboratory December 6
Well water
University water
Materials
4 bottles for sample storage.
Masking tape for labeling containers
Thermometer for measuring the temperature of the samples
Graduated beakers for measuring the volume of samples
Multiparameter to measure pH, electrical conductivity, and dissolved oxygen.
Distilled water
Notebook for notes
Clothing
Apron or cloth
Cofia
Mask (face mask)
Gloves
5.2. Procedure
Listen carefully to the instructor's instructions about the
functioning of the multiparameter.
Place the water samples in labeled beakers.
Ensure that the multiparameter is properly calibrated.
Introduce the electrode to measure pH, temperature, dissolved oxygen, and
electrical conductivity in each of the samples.
After each measurement, clean the used electrodes with water
distilled.
Take note of the results of each measurement in Table 1.
Create a flowchart of the procedure.
Carry out the analysis of the results and draw conclusions.
Compare the results obtained with the current regulations.
Flowchart of the Procedure
START
p H
Introduce the electrode in each one
temperature
sample
dissolved oxygen
electrical conductivity
Carry out the analysis of the
results and formulate conclusions
THE END
Table No. 1.
Analysis of the waters with the multiparameter
TYPE OF pH VOLUME TEMPERATURE OXYGEN Conductivity
No SAMPLE DISSOLVED ELECTRIC
Fresh river water
1 collected. 200 ml 11°C 1.43 785
7.47
River water
2 collected on the day 200 ml 7.63 6.2°C 1.31 617
November 29.
3 Well water. 200 ml 7.53 8.6°C 0.73 1073
4 Water from the 200 ml 8.19 7.6°C 1.40 172.8
university.
Objective 2. Compare the results obtained with the current regulations.
According to DS N° 031-2010-SA, regulation on the quality of water for consumption
Human aims to guarantee its safety, preventing risk factors.
sanitary facilities, as well as protecting and promoting the health and well-being of the population.
The chemical parameters that affect the aesthetic and organoleptic quality of water
for human consumption, they must not exceed the concentrations or values indicated in
table 2 of this Regulation.
Table No. 2.
Maximum permissible limits for organoleptic quality parameters
ELECTRICAL CONDUCTIVITY
When evaluating the results of electrical conductivity according to regulation DS No. 031-
2010-SA, which establishes a maximum permissible limit of 1,500, we can observe the
values obtained in the measurements taken:
a) Freshly collected river water (11°C): 785 (within the permitted range).
b) River water collected on November 29 (6.2°C): 617 (within the range
allowed).
c) Well water (8.6°C): 1073 (within the permitted range).
d) University water (7.6°C): 172.8 (within the permitted range).
According to the results, all samples meet the limits
established by regulations regarding electrical conductivity. It is important
highlight that the values obtained are below the maximum permissible limit of
1,500 established by DS N° 031-2010-SA.
VII. CONCLUSIONS
VIII. RECOMMENDATIONS
Familiarize yourself with the relevant local or international regulations for the
measurements being carried out
Ensure that the samples are taken representatively and that they are not
contaminated.
Record the environmental conditions during the measurements, such as the temperature
environment and weather conditions.
Consider participating in external quality control programs or proficiency testing.
These programs allow you to compare your results with those of other laboratories and
evaluate the precision and accuracy of your measurements.
Perform regular maintenance on the measuring equipment to ensure its proper functioning.
operation. This includes cleaning, calibration, and repair as necessary.
necessary.
IX. BIBLIOGRAPHIC REFERENCE
Escobar, J. (2002). The pollution of rivers and its effects on coastal areas and the sea.
InSeries natural resources and infrastructure (Vol. 50).
Oikawa, S., Tsuda, M., Okamura, Y., & Urabe, T. (1984). Characterization of waters
residuals and the biochemical oxygen demand. Journal of the American Chemical
Society,106(22), 6751–6755. [Link]
Pan American Health Organization. (2012). Study of the quality of sources used
for human consumption and mitigation plan for contamination from domestic use and
agrochemicals in Apurimac and Cusco. Ecofluidos Engineers S.A., 105. Retrieved from
Unable to access external documents or websites.
Sánchez, O. F.
Zahariev, T., Anastassov, V., Girov, K., Goranova, E., Grozdinski, L., Kniajev, V., &
Stankev, M. (2009). PHYSICAL-CHEMICAL ASPECTS OF WATER QUALITY.
International Angiology, 28(4), 303–310.
X. ANNEXES
Figure 3. Insert the electrode to measure pH, temperature, dissolved oxygen and
electrical conductivity in each of the samples.
Figure 4. Temperature results for each sample
b) What methods exist for determining pH? Indicate which ones are and
explain them.
There are several methods to determine the pH of a solution. Some of the
the most common methods are:
Indicator paper: It consists of the use of papers impregnated with
pH indicators that change color according to the pH of the solution.
The paper is immersed in the sample and the resulting color is compared with
a reference scale.
Glass electrodes: A specific glass electrode is used for
measure the hydrogen potential in a solution. The generated signal is
convert it into a pH value. This method is common in instruments of
laboratory and portable devices.
Liquid chemical indicators: Solutions containing are used.
pH indicators that change color in the presence of acids or bases.
The indicator is added to the sample and the color change is observed for
determine the pH.
Ion-selective electrodes (ISE): It is similar to the electrodes of
glass, but specific for hydrogen ions. It is based on the measurement of
potential generated by the difference in ion concentration of
hydrogen in the solution.
Electronic pH meters: A glass electrode or a
ISE together with an electronic meter that converts the signal into a value
pH numerical. These meters are precise and widely used.
in laboratory environments.
Equivalence point in a titration: In acid-base titrations, the
point at which the amount of acid is equal to the amount of base added
is associated with a specific pH. The determination of the pH at this point
Can you indicate the pH value of the solution.
c) Research how to measure the flow rate in a river
Measuring the flow of a river is important to understand and monitor the flow of
water at a specific moment. There are several methods to measure the flow.
and the choice of method will depend on factors such as the required accuracy, the
available budget and the specific conditions of the river. Here are some
common techniques:
Floater Method: This method involves throwing a floater into the river and
measure the time it takes to cover a known distance. Known
the speed and the cross-sectional area of the river can be calculated
the flow.
Gauges: Gauges are structures installed in the river that
they allow measuring the flow of water. Some common types include the
Parshall flume and Venturi flume. These structures measure the height
from the water and use specific equations to estimate the flow rate.
Wind vanes: Wind vanes are devices that measure the speed of the wind.
water on the surface of the river. Multiplying the speed by the area of the
cross-section, the flow rate can be calculated.
Controlled Discharge Current Method: This method involves
control and measure the amount of water released from a dam or structure
similar. Measuring the speed and the cross-sectional area, one
can determine the flow.
Ultrasonic Meters: These devices use ultrasonic waves.
to measure the speed of water. The speed is combined with the area of
the cross-section to calculate the flow.
Mathematical Modeling: Mathematical models can be used to
estimate the flow rate based on topographic data, conditions
hydraulics and water level measurements.
Pressure sensors: Pressure sensors measure water pressure
at a specific point and can be used to calculate the water level,
which are then used to determine the flow rate.
Visual Observation and Aerial Photographs: The visual observation of the
river and the analysis of aerial photographs can also provide
information about the flow, especially in areas where it cannot be
use direct methods.
e) What are the time intervals between sampling or type of sample and the
water analysis?
The time intervals between water sampling and analysis vary depending on the
nature of the parameters and the objectives of monitoring. Some parameters
can be analyzed immediately at the sampling site, while others
they require time for processing in the laboratory. The availability of
results can range from a few minutes, in the case of on-site analysis, to several
days in more detailed analysis. The proper preservation of samples and the
Planning of regular sampling programs are important considerations
to ensure the accuracy of the results.
to be realized.
Water sampling from different sources, such as distribution systems, wells,
rivers, lakes, and reservoirs require a careful and standardized approach to
ensure the representativeness of the samples and the accuracy of the results.
Here are some general considerations and guidelines for water sampling.
in each type of source:
Sampling in Distribution Systems:
Selection of Sampling Points: Choose sampling points
representatives from various areas of the distribution system to obtain
a complete picture of the water quality in the network.
Avoid Contaminations during Sampling: Make sure not to
contaminate the samples during collection. Use methods of
aseptic sampling and carefully clean the equipment before each collection
as a sample.
Sampling in Constant Flows: Perform sampling during periods
of constant flow to obtain more representative samples.
Sampling in Wells:
Disinfection of Equipment: Disinfect the sample equipment before use.
used to avoid contamination of the samples.
Sampling Depth: Conduct sampling at different
depths if it is relevant to assess water quality in
different strata of the aquifer.
Sampling in Rivers or Currents:
Strategic Location of Sampling Points: Select sites
representatives along the river, considering the variations in the
flow velocity, depth and proximity to sources of
contamination.
Sampling in Different Climatic Conditions: Carry out samplings in
different climatic conditions to capture seasonal variations.
In-Depth Sampling: If relevant, carry out the sampling at
different depths to assess possible quality gradients of the
water.