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Measuring Optical Rotation with a Polarimeter

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7 views1 page

Measuring Optical Rotation with a Polarimeter

Uploaded by

sabirbatazai
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Guide to Measuring Optical Rotation Using a Polarimeter

1. Set the Reference Point:

- Open the shield and place a test tube filled with distilled water into the sample chamber.

- Ensure the bubble trap is facing upward.

- Close the shield and rotate the dial vernier until both halves of the view are equally bright.

- Note the measured angle.

2. Improve Accuracy:

- Rotate the dial clockwise and anticlockwise to find equal brightness again.

- Record both values and calculate their average. This is your reference point.

3. Measure the Sample:

- Replace the distilled water with your sample solution.

- Rotate the dial again to find equal brightness.

- Record the angle of rotation.

4. Calculate Actual Rotation:

- If the measured angle is positive, the substance is dextrorotatory:

Subtract the reference point from the measured value.

- If the measured angle is negative, the substance is levorotatory:

Subtract 180 degrees from the measured value.

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